A New Workflow for Whole-Genome Sequencing of Single Human Cells

Human Mutation

Volumn 35, Issue 10, 2014, Pages 1260-1270

  Binder, Vera ^a,c   Bartenhagen, Christoph ^b   Okpanyi, Vera ^a   Gombert, Michael ^a   Moehlendick, Birte ^a   Behrens, Bianca ^a   Klein, Hans Ulrich ^b   Rieder, Harald ^a   Ida Krell, Pina Fanny ^a   Dugas, Martin ^b   Stoecklein, Nikolas Hendrik ^a   Borkhardt, Arndt ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b UNIVERSITY OF MÜNSTER   (Germany)

^c BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

Allele dropout rate; CNV; LOH; Single cell; Whole genome amplification

Indexed keywords

CYCLIN DEPENDENT KINASE INHIBITOR 2A; GENOMIC DNA; MUTL PROTEIN HOMOLOG 1; PROTEIN P53;

ARTICLE; CDKN2A GENE; COPY NUMBER VARIATION; GENE DELETION; GENE FREQUENCY; GENETIC HETEROGENEITY; HETEROZYGOSITY LOSS; HUMAN; HUMAN CELL; HUMAN GENOME; INTERMETHOD COMPARISON; MLH1 GENE; POINT MUTATION; POLYMERASE CHAIN REACTION; SINGLE CELL ANALYSIS; TUMOR SUPPRESSOR GENE; WHOLE GENOME SEQUENCING; WORKFLOW; CHROMOSOME ABERRATION; DNA SEQUENCE; HIGH THROUGHPUT SEQUENCING; PROCEDURES; REPRODUCIBILITY;

CHROMOSOME ABERRATIONS; DNA COPY NUMBER VARIATIONS; GENE FREQUENCY; GENETIC HETEROGENEITY; GENOME, HUMAN; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; LOSS OF HETEROZYGOSITY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SEQUENCE ANALYSIS, DNA; SINGLE-CELL ANALYSIS; WORKFLOW;

EID: 84908875580     PISSN: 10597794     EISSN: 10981004     Source Type: Journal    
DOI: 10.1002/humu.22625     Document Type: Article

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