Microsatellite high-resolution melting (SSR-HRM) analysis for genotyping and molecular characterization of an Olea europaea germplasm collection

Plant Genetic Resources: Characterisation and Utilisation

Volumn 12, Issue 3, 2014, Pages 273-277

  Xanthopoulou, Aliki ^a,b   Ganopoulos, Ioannis ^a,b   Koubouris, Georgios ^c   Tsaftaris, Athanasios ^a,b   Sergendani, Chrysa ^c   Kalivas, Apostolos ^d   Madesis, Panagiotis ^a  

^a INFORMATION TECHNOLOGIES INSTITUTE   (Greece)

^b ARISTOTLE UNIVERSITY OF THESSALONIKI   (Greece)

^c NAGREF Insitute of Olive Tree And Subtropical Plants Of Chania   (Greece)

^d INSTITUTE OF TECHNOLOGY OF AGRICULTURAL PRODUCTS   (Greece)

Author keywords

authentication; Gene Bank collection; genotyping; olive; SSR HRM analysis

Indexed keywords

EID: 84908665181     PISSN: 14792621     EISSN: 1479263X     Source Type: Journal    
DOI: 10.1017/S147926211400001X     Document Type: Article

References (15)

1. Barranco D, Cimato A, Fiorino P, Rallo L, Touzani A, Castaneda C, Serafini F and Trujillo I (2000) Catalogo Mundial de las Variedades de Olivo. Consejo Oleícola Internacional. Madrid: L.R. Cuellar, p. 360.
2. Cipriani G, Marrazzo MT, Marconi R, Cimato A and Testolin R (2002) Microsatellite markers isolated in olive (O. europaea L.) are suitable for individual fingerprinting and reveal polymorphism within ancient cultivars. Theoretical and Applied Genetics 104: 223-228.
3. Distefano G, Caruso M, La Malfa S, Gentile A and Wu S-B (2012) High resolution melting analysis is a more sensitive and effective alternative to gel-based platforms in analysis of SSR: an example in Citrus. PloS one 7: e44202.
4. Donini P, Sarri V, Baldoni L, Porceddu A, Cultrera NGM, Contento A, Frediani M, Belaj A, Trujillo I and Cionini PG (2006) Microsatellite markers are powerful tools for discriminating among olive cultivars and assigning them to geographically defined populations. Genome 49: 1606-1615.
5. Evans KM, Patocchi A, Rezzonico F, Mathis F, Durel CE, Fernandez-Fernandez F, Boudichevskaia A, Dunemann F
6. Stankiewicz-Kosyl M and Gianfranceschi L (2011) Genotyp-ing of pedigreed apple breeding material with a genome-covering set of SSRs: trueness-to-type of cultivars and their parentages. Molecular Breeding 28: 535-547.
7. Ganopoulos I, Argiriou A and Tsaftaris A (2011a) Microsatellite high resolution melting (SSR-HRM) analysis for authenticity testing of protected designation of origin (PDO) sweet cherry products. Food Control 22: 532-541.
8. Ganopoulos IV, Kazantzis K, Chatzicharisis I, Karayiannis I and Tsaftaris AS (2011b) Genetic diversity, structure and fruit trait associations in Greek sweet cherry cultivars using microsatellite based (SSR/ISSR) and morpho-physiological markers. Euphytica 181: 237-251.
9. Marra FP, Caruso T, Costa F, Di Vaio C, Mafrica R and Marchese A (2013) Genetic relationships, structure and parentage simulation among the olive tree (Olea europaea L. subsp. europaea) cultivated in Southern Italy revealed by SSR markers. Tree Genetics & Genomes: 1-13.
10. Reed GH and Wittwer CT (2004) Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis. Clinical Chemistry 50: 1748-1754.
11. Roubos K, Moustakas M and Aravanopoulos FA (2011) Molecular identification of Greek olive (Olea europaea) cultivars based on microsatellite loci. Genetics and Molecular Research 9: 1865-1876.
12. Sefc KM, Lopes MS, Mendonca D, Santos MRD, Machado LM and Machado ADC (2000) Identification of microsatellite loci in olive (Olea europaea) and their characterization in Italian and Iberian olive trees. Molecular Ecology 9: 1171-1173.
13. Stambuk S, Sutlović D, Bakarić P, Petricević S and Andelinović S (2007) Forensic botany: potential usefulness of microsatel-lite-based genotyping of Croatian olive (Olea europaea L.) in forensic casework. Croatian Medical Journal 48: 556-562.
14. Therios I (2009) Olives. In: Crop Production Science in Horticulture Series No. 18. Wallingford: CABI Publishing, p. 336.
15. Xanthopoulou A, Ganopoulos I, Tsaballa A, Nianiou-Obeidat I, Kalivas A, Tsaftaris A and Madesis P (2013) Summer squash identification by high-resolution-melting (HRM) analysis using gene-based EST-SSR molecular markers. Plant Molecular Biology Reporter: 1-11.