Picrosirius Red Staining: A Useful Tool to Appraise Collagen Networks in Normal and Pathological Tissues

Journal of Histochemistry and Cytochemistry

Volumn 62, Issue 10, 2014, Pages 751-758

  Lattouf, Raed ^a   Younes, Ronald ^a   Lutomski, Didier ^b   Naaman, Nada ^a   Godeau, Gaston ^c   Senni, Karim ^a   Changotade, Sylvie ^b  

^a SAINT JOSEPH UNIVERSITY   (Lebanon)

^b UMR 7244   (France)

^c UNIVERSITÉ PARIS DESCARTES   (France)

Author keywords

birefringence; collagen fibers; Ehlers Danlos syndrome; picrosirius red staining polarized light

Indexed keywords

COLLAGEN TYPE 3; AZO COMPOUND; C.I. DIRECT RED 80; COLLAGEN;

ADULT; ARTICLE; BASEMENT MEMBRANE; CASE REPORT; CONNECTIVE TISSUE; CONTROLLED STUDY; DERMOEPIDERMAL JUNCTION; EHLERS DANLOS SYNDROME; EPIDERMIS; EXTRACELLULAR MATRIX; FEMALE; HUMAN; HUMAN TISSUE; IMMUNODETECTION; MALE; MICROSCOPY; PICROSIRIUS RED STAINING; SKIN BIOPSY; SKIN FIBROBLAST; STAINING; TISSUE SECTION; YOUNG ADULT; CYTOLOGY; LIGHT; METABOLISM; PATHOLOGY; PROCEDURES; SKIN;

ADULT; AZO COMPOUNDS; COLLAGEN; CONNECTIVE TISSUE; EHLERS-DANLOS SYNDROME; FEMALE; HUMANS; LIGHT; MALE; MICROSCOPY; SKIN; STAINING AND LABELING; YOUNG ADULT;

EID: 84908641540     PISSN: 00221554     EISSN: 15515044     Source Type: Journal    
DOI: 10.1369/0022155414545787     Document Type: Article

References (35)

1. Bayounis AM,Alzoman HA,Jansen JA,Babay N.Healing of peri-implant tissues after flapless and flapped implant installation.J Clin Periodontol. 2011;38:754-761
2. Berton A,Godeau G,Emonard H,Baba K,Bellon P,Hornebeck W,Bellon G.Analysis of the ex vivo specificity of human gelatinases A and B towards skin collagen and elastic fibers by computerized morphometry.Matrix Biol. 2000;19:139-148
3. Binnebösel M,Klink CD,Otto J,Conze J,Jansen PL,Anurov M,Schumpelick V,Junge K.Impact of mesh positioning on foreign body reaction and collagenous ingrowth in a rabbit model of open incisional hernia repair.Hernia. 2010;14:71-77
4. Born M,Wolf EPrinciples of optics: Electromagnetic theory of propagation, interference and diffraction of light. 1999. Principles of optics: Electromagnetic theory of propagation, interference and diffraction of light, 7th edition, Cambridge, UK: Cambridge University Press, pp 49, 95-103, 843.Cambridge, UK: Cambridge University Press; 1999:49-103.
5. Cavallo JA,Roma AA,Jasielec MS,Ousley J,Creamer J,Pichert MD,Baalman S,Frisella MM,Matthews BD,Deeken CR.Remodeling characteristics and collagen distribution in synthetic mesh materials explanted from human subjects after abdominal wall reconstruction: an analysis of remodeling characteristics by patient risk factors and surgical site classifications.Surg Endosc. 2014;28:1852-1865
6. Chaussain Miller C,Septier D,Bonnefoix M,Lecolle S,Lebreton-Decoster C,Coulomb B,Pellat B,Godeau G.Human dermal and gingival fibroblasts in a three-dimensional culture: a comparative study on matrix remodeling.Clin Oral Investig. 2002;6:39-50
7. Chiodo AA,Sillence DO,Cole WG,Bateman JF.Abnormal type III collagen produced by an exon-17-skipping mutation of the COL3A1 gene in Ehlers-Danlos syndrome type IV is not incorporated into the extracellular matrix.Biochem J. 1995;311:939-943
8. Coen M,Menegatti E,Salvi F,Mascoli F,Zamboni P,Gabbiani G,Bochaton-Piallat ML.Altered collagen expression in jugular veins in multiple sclerosis.Cardiovasc Pathol. 2013;22:33-38
9. Coleman R.Picrosirius red staining revisited.Acta Histochem. 2011;113:231-233
10. Dayan D,Hiss Y,Hirshberg A,Bubis JJ,Wolman M.Are the polarization colors of picrosirius red-stained collagen determined only by the diameter of the fibers?.Histochemistry. 1989;93:27-29
11. Ejeil AL,Gaultier F,Igondjo-Tchen S,Senni K,Pellat B,Godeau G,Gogly B.Are cytokines linked to collagen breakdown during periodontal disease progression?.J Periodontol. 2003;74:196-201
12. Ghomrasseni S,Dridi M,Bonnefoix M,Septier D,Gogly G,Pellat B,Godeau G.Morphometric analysis of elastic skin fibres from patients with: cutis laxa, anetoderma, pseudoxanthoma elasticum, and Buschke-Ollendorff and Williams-Beuren syndromes.J Eur Acad Dermatol Venereol. 2001;15:305-311
13. Ghomrasseni S,Dridi M,Gogly B,Bonnefoix M,Vabres P,Venencie PY,Pellat B,Godeau G.Anetoderma: an altered balance between metalloproteinases and tissue inhibitors of metalloproteinases.Am J Dermatopathol. 2002;24:118-129
14. Gogly B,Godeau G,Gilbert S,Legrand JM,Kut C,Pellat B,Goldberg M.Morphometric analysis of collagen and elastic fibers in normal skin and gingival in relation to age.Clin Oral Investig. 1997;1:147-152
15. Hamel BC,Pals G,Engels CH,van den Akker E,Boers GH,van Dongen PW,Steijlen PM.Ehlers-Danlos syndrome and type III collagen abnormalities: a variable clinical spectrum.Clin Genet. 1998;53:440-446
16. Holbrook KA,Byers PH.Skin is a window on heritable disorders of connective tissue.Am J Med Genet. 1989;34:105-121
17. Holbrook KA,Byers PH,Pinnell SR.The structure and function of dermal connective tissue in normal individuals and patients with inherited connective tissue disorders.Scan Electron Microsc. 1982; (Pt 4): 1731-1744
18. Holbrook KA,Byers PH.Structural abnormalities in the dermal collagen and elastic matrix from the skin of patients with inherited connective tissue disorders.J Invest Dermatol. 1982;79:7s-16s
19. Junqueira LC,Bignolas G,Brentani RR.Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections.Histochem J. 1979;11:447-455
20. Junqueira LC,Roscoe JT.Reduced collagen content and fibre bundle disorganization in skin biopsies of patients with Ehlers-Danlos syndrome.Histochem J. 1985;17:1197-1202
21. Malkusch W,Rehn B,Bruch J.Advantages of Sirius Red staining for quantitative morphometric collagen measurements in lungs.Exp Lung Res. 1995;21:67-77
22. Montes GS,Bezerra MS,Junqueira LCUltrastructure of the connective tissue matrix. Ruggeri AMotta PM, ed. Boston: Martinus Nijhoff Publishers; 1984:65-88.
23. Montes GS,Junqueira LC.The use of the Picrosirius-polarization method for the study of the biopathology of collagen.Mem Inst Oswaldo Cruz. 1991;86:1-11
24. Peeters E,De Hertogh G,Junge K,Klinge U,Miserez M.Skin as marker for collagen type I/III ratio in abdominal wall fascia.Hernia. 2013;:
25. Pepin MG,Byers PHGeneReviews™. Seattle (WA). Pagon RAAdam MP, ed. Seattle: University of Washington; 1999:
26. Piérard GE.Sirius red polarization method is useful to visualize the organization of connective tissues but not the molecular composition of their fibrous polymers.Matrix. 1989;9:68-71
27. Ricard-Blum S.The collagen family.Cold Spring Harb Perspect Biol. 2011;3:a004978
28. Rich L,Whittaker P.Collagen and picrosirius red staining: a polarized light assessement of fibrillar hue and spatial distribution.Braz J Morphol Sci. 2005;22:97-104
29. Ritelli M,Dordoni C,Venturini M,Chiarelli N,Quinzani S,Traversa M,Zoppi N,Vascellaro A,Wischmeijer A,Manfredini E,Garavelli L,Calzavara-Pinton P,Colombi M.Clinical and molecular characterization of 40 patients with classic Ehlers-Danlos syndrome: identification of 18 COL5A1 and 2 COL5A2 novel mutations.Orphanet J Rare Dis. 2013;8:58
30. Connective Tissue and Its Heritable Disorders: Molecular, Genetic, and Medical Aspects. 2003. Connective Tissue and Its Heritable Disorders: Molecular, Genetic, and Medical Aspects, 2nd edition, New York: Wiley-Liss, Inc.Royce PMSteinmann B, ed. New York: Wiley-Liss, Inc; 2003:
31. Sansilvestri-Morel P,Fioretti F,Rupin A,Senni K,Fabiani JN,Godeau G,Verbeuren TJ.Comparison of extracellular matrix in skin and saphenous veins from patients with varicose veins: does the skin reflect venous matrix changes?.Clin Sci (Lond). 2007;112:229-239
32. Séguier S,Godeau G,Brousse N.Collagen fibers and inflammatory cells in healthy and diseased human gingival tissues: a comparative and quantitative study by immunohistochemistry and automated image analysis.J Periodontol. 2000;71:1079-1085
33. Séguier S,Gogly B,Bodineau A,Godeau G,Brousse N.Is collagen breakdown during periodontitis linked to inflammatory cells and expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human gingival tissue?.J Periodontol. 2001;72:1398-1406
34. Steinmann B,Royce PM,Superti-Furga AConnective Tissue and Its Heritable Disorders: Molecular, Genetic, and Medical Aspects. 2002. ‘The Ehlers-Danlos syndrome’ in RoycePMSteinmannB (eds.) Connective Tissue and Its Heritable Disorders: Molecular, Genetic, and Medical Aspects, 2nd edition, New York: Wiley-Liss, Inc. pp 437-523.Royce PMSteinmann B, ed. New York: Wiley-Liss, Inc; 2002:437-523.
35. Symoens S,Syx D,Malfait F,Callewaert B,De Backer J,Vanakker O,Coucke P,De Paepe A.Comprehensive molecular analysis demonstrates type V collagen mutations in over 90% of patients with classic EDS and allows to refine diagnostic criteria.Hum Mutat. 2012;33:1485-1493