Isolation of a circulating CD45-, CD34dim cell population and validation of their endothelial phenotype

Thrombosis and Haemostasis

Volumn 112, Issue 4, 2014, Pages 770-780

  Tropea, Margaret M ^a   Harper, Bonnie J A ^a   Graninger, Grace M ^a   Phillips, Terry M ^b   Ferreyra, Gabriela ^a   Mostowski, Howard S ^d   Danner, Robert L ^a   Suffredini, Anthony F ^a   Solomon, Michael A ^a,c  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING   (United States)

^c NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^d CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

Author keywords

Circulating endothelial cells; Flow cytometry; Gene expression; Vascular cell markers; Viability

Indexed keywords

CD14 ANTIGEN; CD146 ANTIGEN; CD19 ANTIGEN; CD3 ANTIGEN; CD31 ANTIGEN; CD34 ANTIGEN; CD45 ANTIGEN; ENDOGLIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; ULEX EUROPAEUS AGGLUTININ; VASCULAR ENDOTHELIAL CADHERIN; VON WILLEBRAND FACTOR; 4',6 DIAMIDINO 2 PHENYLINDOLE; 7-AMINOACTINOMYCIN D; DACTINOMYCIN; INDOLE DERIVATIVE; PLANT LECTIN; RNA; ULEX EUROPAEUS LECTINS;

7-AAD ASSAY; ADULT; ANTIGEN PRESENTATION; ARTICLE; CELL ISOLATION; CELL POPULATION; CELL VIABILITY; CIRCULATING ENDOTHELIAL CELL; ENDOTHELIUM CELL; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; HUMAN CELL; IMMUNOAFFINITY CHROMATOGRAPHY; IMMUNOCHEMISTRY; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOHISTOCHEMISTRY; MICROSCOPY; MONONUCLEAR CELL; NORMAL HUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA ISOLATION; UMBILICAL VEIN ENDOTHELIAL CELL; ANALOGS AND DERIVATIVES; BLOOD; CELL MEMBRANE; CELL NUCLEUS; CELL SEPARATION; CELL SURVIVAL; CHEMISTRY; CYTOLOGY; FLUORESCENCE MICROSCOPY; METABOLISM; MIDDLE AGED; PHENOTYPE; PILOT STUDY;

ADULT; ANTIGENS, CD146; ANTIGENS, CD31; ANTIGENS, CD34; ANTIGENS, CD45; CELL MEMBRANE; CELL NUCLEUS; CELL SEPARATION; CELL SURVIVAL; DACTINOMYCIN; ENDOTHELIAL CELLS; FLOW CYTOMETRY; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; INDOLES; LEUKOCYTES, MONONUCLEAR; MICROSCOPY; MICROSCOPY, FLUORESCENCE; MIDDLE AGED; PHENOTYPE; PILOT PROJECTS; PLANT LECTINS; RNA; VON WILLEBRAND FACTOR;

EID: 84908506942     PISSN: 03406245     EISSN: None     Source Type: Journal    
DOI: 10.1160/TH14-01-0043     Document Type: Article

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