A six-colour flow cytometric method for simultaneous detection of cell phenotype and apoptosis of circulating endothelial cells

Scandinavian Journal of Clinical and Laboratory Investigation

Volumn 69, Issue 3, 2009, Pages 433-438

  Mariucci, S ^a   Rovati, B ^a   Chatzileontiadou, S ^a   Bencardino, K ^b   Manzoni, M ^a   Delfanti, S ^a   Danova, M ^a  

^a FONDAZIONE IRCCS POLICLINICO SAN MATTEO   (Italy)

^b HSR Scientific Institute   (Italy)

Author keywords

Annexin V; Flow cytometry; Immunophenotype; Peripheral blood; Rare events analysis

Indexed keywords

DACTINOMYCIN; LIPOCORTIN 5; MONOCLONAL ANTIBODY; 7 AMINODACTINOMYCIN; 7-AMINOACTINOMYCIN D; DRUG DERIVATIVE;

ADULT; APOPTOSIS; ARTICLE; BLOOD SAMPLING; BLOOD VESSEL INJURY; CLINICAL STUDY; ENDOTHELIUM CELL; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN EXPERIMENT; MALE; NORMAL HUMAN; PHENOTYPE; PRIORITY JOURNAL; STEM CELL; VASCULARIZATION; BLOOD CELL; COLOR; CYTOLOGY; EVALUATION; METHODOLOGY; MIDDLE AGED; SENSITIVITY AND SPECIFICITY;

ADULT; ANNEXIN A5; APOPTOSIS; BLOOD CELLS; COLOR; DACTINOMYCIN; ENDOTHELIAL CELLS; FEMALE; FLOW CYTOMETRY; HUMANS; MALE; MIDDLE AGED; PHENOTYPE; SENSITIVITY AND SPECIFICITY;

EID: 67649690990     PISSN: 00365513     EISSN: 15027686     Source Type: Journal    
DOI: 10.1080/00365510802673175     Document Type: Article

References (23)

1. Duda DG, Cohen KS, Scadden DT, Jain RK. A protocol for phenotypic detection and enumeration of circulating endothe-lial cells and circulating progenitor in human blood. Nature Protocols 2007;2:805-10.
2. Strijbos MH, Gratama JW, Kraan J, Lamers CH, den Bakker MA, Sleijfer S. Circulating endothelial cells in oncology: pitfalls and promises. Br J Cancer 2008;98:1731-5.
3. Goon PKY, Boos CJ, Stonelake PS, Blann AD, Lip GYH. Detection and quantification of mature circulating endothelial cells using flow cytometry and immunomagnetics beads: a methodological comparison. Thromb Haemost 2006;96:45-52.
4. George F, Brouqui P, Boffa MC, Drancourt M, Brisson C, Raoult D, Sampol J. Demonstration of Rickettsia conorii-induced endothelial injury in vivo by measuring circulating endothelial cells, thrombomodulin, and von Willebrand factor in patients with Mediterranean spotted fever. Blood 1993;82:2109-16.
5. Mancuso P, Burlini A, Pruneri G, Goldhirsch A, Martinelli G, Bertolini F. Resting and activated endothelial cells are increased in the peripheral blood of cancer patients. Blood 2001;97:3658-61.
6. Mutunga M, Fulton B, Bullock R, Batchelor A, Gascoigne A, Gillespie JI, Baudouin SV. Circulating endothelial cells in patients with septic shock. AJRCCM 2001;163:195-200.
7. Bull TM, Golpon H, Hebbel RP, Solovey A, Cool CD, Tuder RM, Geraci MW, Voelkel NF. Circulating endothelial cells in pulmonary hypertension. Thromb Haemost 2003;90:698-703.
8. Del Papa N, Colombo G, Fracchiolla N, Moronetti LM, Ingegnoli F, Maglione W, Comina DP, Vitali C, Fantini F, Cortelezzi A. Circulating endothelial cells as a marker of ongoing vascular disease in systemic sclerosis. Arthritis Rheum 2004;50:1296-304.
9. Nakatani K, Takeshita S, Tsujimoto H, Kawamura Y, Tokutomi T, Sekine I. Circulating endothelial cells in Kawasaki disease. Clin Exp Immunol 2003;361:536-40.
10. Khan SS, Solomon MA, McCoy Jr JP. Detection of circulating endothelial cells and endothelial progenitor cells by flow cytometry. Cytometry Part B. Clin Cytometry 2005;64:1-8.
11. El Solh AA, Akinnusi ME, Baddoura FH, Mankowski CR. Endothelial cells apoptosis in obstructive sleep apnea. Am J Respir Crit Care Med 2007;175:1186-91.
12. Bertolini F, Mancuso P, Shaked Y, Kerbel RS. Molecular and cellular biomarkers for angiogenesis in clinical oncology. Drug Discovery Today 2007;12:806-12.
13. Bertolini F. Chemotherapy and the tumour microenviron-ment: the contribution of circulating endothelial cells. Cancer Metastasis Rev 2008;27:95-101.
14. Anonymous. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. J Postgrad Med 2002;48:206-8.
15. Whitby L, Granger W, Storie I, Goodfellow K, Sawle A, Reilly JT, Barnett D. Quality control of CD4+ T-lymphocyte enumeration: results from 9 years of the United Kingdom National External Quality Assessment Scheme for Immune Monitoring (1993-2001). Cytometry 2002;50:102-10.
16. Körbling M, Reuben JM, Gao H, Lee B-N, Harris DM, Cogdell D, Giralt SA, Khouri IF, Saliba RM, Champlin RE, Zhang W, Estrov Z. Recombinant human granulocyte-colony-stimulating factor-mobilized and apheresis-collected endothe-lial progenitor cells: a novel blood cells component for therapeutic vasculogenesis. Transfusion 2006;46:1795-802.
17. Mariucci S, Rovati B, Bencardino K, Manzoni M, Danova M. Flow cytometric detection of circulating endothelial cells and endothelial progenitor cells in healthy subjects. Int J Lab Haematol 2008. In press.
18. Jacques N, Vimond N, Conforti R, Griscielli F, Lecluse Y, Laplanche A, Malka D, Vielh P, Farace F. Quantification of circulating mature endothelial cells using a whole blood four-color flow cytometric assay. J Immunol Methods 2008;15;337:132-43.
19. Redondo S, Hristov M, Gordillo-Moscoso AA, Ruiz E, Weber C, Tejerina T. High reproducible flow cytometric endothelial progenitor cell determination in human peripheral blood as CD34+/CD144+/CD32 lymphocyte sub-population. J Immunol Methods 2008;335:21-7.
20. Van Craenenbroeck EMF, Conraads VMA, Van Bockstaele DR, Haine SE, Vermeulen K, Van Tendeloo VG, Vrints CJ, Hoymans VJ. Quantification of circulating endothelial progenitor cells: a methodological comparison of six flow cytometric approaches. J Immunol Methods 2008;332:31-40.
21. McCarthy DA, Macey MG. Quality control in flow cytome-try. Cytometric analysis of cell phenotype and function. Cambridge: Cambridge University Press; 2001. p. 74.
22. Wirezbwska A, Robak T, Krawczyńska A, Pluta A, Wrzesień-Kuś A, Cebula B, Robak E, Smolewski P. Kinetics and apoptotic profile of circulating endothelial cells as prognostic factors for induction treatment failure in newly diagnosed acute myeloid leukemia patients. Ann Hematol 2008;87: 97-106.
23. Go RS, Jobe DA, Asp KE, Callister SM, Mathiason MA, Meyer LA, Bottner WA, Cole CE, Farner JP, Frisby KA. Circulating endothelial cells in patients with chronic lympho-cytic leukemia. Ann Hematol 2008;87:369-73.