Rapid one-step purification of single-cells encapsulated in alginate microcapsules from oil to aqueous phase using a hydrophobic filter paper: Implications for single-cell experiments

Biotechnology Journal

Volumn 9, Issue 10, 2014, Pages 1233-1240

  Lee, Do Hyun ^a   Jang, Miran ^a   Park, Je Kyun ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Droplet based microfluidics; Hydrogel microcapsule; Microcapsule purification; Single cell encapsulation

Indexed keywords

ALGINATE; BIOCOMPATIBILITY; CELL CULTURE; CELL PROLIFERATION; CENTRIFUGATION; FILTERS (FOR FLUIDS); HYDROGELS; HYDROPHOBICITY; MAMMALS; MICROSTRUCTURE; PURIFICATION;

ALGINATE MICROCAPSULES; CENTRIFUGAL PURIFICATION; DROPLET-BASED MICROFLUIDICS; LONG-TERM INCUBATION; MICROCAPSULES; MICROCHANNEL NETWORK; ONE-STEP PURIFICATION; SINGLE CELLS;

CELLS;

ALGINIC ACID; OIL; CALCIUM CHLORIDE; GLUCURONIC ACID; HEXURONIC ACID; MICROCAPSULE; OLEIC ACID;

ANALYTIC METHOD; ARTICLE; CANCER CELL LINE; CELL ENCAPSULATION; CELL PROLIFERATION; CHLORELLA VULGARIS; CONTROLLED STUDY; FILTER; HUMAN; HUMAN CELL; HYDROGEL; MICROCAPSULE; MICROFLUIDICS; PURIFICATION; SINGLE CELL ANALYSIS; CELL SEPARATION; CELL SHAPE; CHEMICAL PHENOMENA; CHEMISTRY; CYTOLOGY; DEVICES; EQUIPMENT DESIGN; HEPG2 CELL LINE; ISOLATION AND PURIFICATION; MCF 7 CELL LINE; MICROFLUIDIC ANALYSIS; PAPER; PHYSIOLOGY; PROCEDURES;

ALGINATES; CALCIUM CHLORIDE; CAPSULES; CELL SEPARATION; CELL SHAPE; CHLORELLA VULGARIS; EQUIPMENT DESIGN; GLUCURONIC ACID; HEP G2 CELLS; HEXURONIC ACIDS; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MCF-7 CELLS; MICROFLUIDIC ANALYTICAL TECHNIQUES; OLEIC ACID; PAPER;

EID: 84908485827     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201400319     Document Type: Article

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