A Simple and Fast Protocol for the Protein Complex Immunoprecipitation (Co-IP) of Effector: Host Protein Complexes

Methods in Molecular Biology

Volumn 1127, Issue , 2014, Pages 195-211

  Steinbrenner, Jens ^a   Eldridge, Matthew ^a   Tomé, Daniel F A ^a   Beynon, Jim L ^a  

^a NONE

Author keywords

Co IP; Effector; Hyaloperonospora arabidopsidis; Oomycetes; Transient expression

Indexed keywords

MULTIPROTEIN COMPLEX; GREEN FLUORESCENT PROTEIN; PRIMER DNA; VEGETABLE PROTEIN;

AGROBACTERIUM TUMEFACIENS; ARABIDOPSIS THALIANA; ARTICLE; CELL POLARITY; CONTROLLED STUDY; CYTOSKELETON; HOST; IMMUNOPRECIPITATION; MOLECULAR CLONING; NICOTIANA BENTHAMIANA; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT; STOP CODON; SUPERNATANT; TRANSIENT EXPRESSION; TWO HYBRID SYSTEM; WESTERN BLOTTING; AGROBACTERIUM; CYTOLOGY; GENE EXPRESSION; GENETIC TRANSFORMATION; HOST PATHOGEN INTERACTION; METABOLISM; METHODOLOGY; TIME; TOBACCO;

AGROBACTERIUM; DNA PRIMERS; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; HOST-PATHOGEN INTERACTIONS; IMMUNOPRECIPITATION; PLANT PROTEINS; TIME FACTORS; TOBACCO; TRANSFORMATION, GENETIC;

EID: 84908482107     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-986-4_16     Document Type: Article

References (34)

1. Boch J, Bonas U (2010) Xanthomonas AvrBs3 family-type III effectors: Discovery and function. Annu Rev Phytopathol 48:419–436
2. Kim JG et al (2008) XopD SUMO protease affects host transcription, promotes pathogen growth, and delays symptom development in xanthomonas-infected tomato leaves. Plant Cell 20:1915–1929
3. Shao F et al (2003) Cleavage of Arabidopsis PBS1 by a bacterial type III effector. Science 301:1230–1233
4. Nomura K et al (2006) A bacterial virulence protein suppresses host innate immunity to cause plant disease. Science 313:220–223
5. Nomura K et al (2011) Effector-triggered immunity blocks pathogen degradation of an immunity-associated vesicle traffic regulator in Arabidopsis. Proc Natl Acad Sci USA 108: 10774–10779
6. Lee AHY et al (2012) A bacterial acetyltransferase destroys plant microtubule networks and blocks secretion. PLoS Pathog 8:e1002523
7. Abramovitch RB et al (2006) Type III effector AvrPtoB requires intrinsic E3 ubiquitin ligase activity to suppress plant cell death and immunity. Proc Natl Acad Sci USA 103:2851–2856
8. Gimenez-Ibanez S et al (2009) AvrPtoB targets the LysM receptor kinase CERK1 to promote bacterial virulence on plants. Curr Biol 19:423–429
9. van Damme M et al (2012) The Irish potato famine pathogen Phytophthora infestans translocates the CRN8 kinase into host plant cells. PLoS Pathog 8:e1002875
10. Bos J et al (2010) Phytophthora infestans effector AVR3a is essential for virulence and manipulates plant immunity by stabilizing host E3 ligase CMPG1. Proc Natl Acad Sci USA 107:9909–9914
11. Bozkurt TO et al (2011) Phytophthora infestans effector AVRblb2 prevents secretion of a plant immune protease at the haustorial interface. Proc Natl Acad Sci USA 108: 20832–20837
12. Saunders DGO et al (2012) Host protein BSL1 associates with Phytophthora infestans RXLR effector AVR2 and the Solanum demissum Immune receptor R2 to mediate disease resistance. Plant Cell 24:3420–3434
13. Mukhtar MS et al (2011) Independently evolved virulence effectors converge onto hubs in a plant immune system network. Science 333:596–601
14. Kerppola TK (2008) Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells. Annu Rev Biophys 37:465–487
15. Gebert M, Dresselhaus T, Sprunck S (2008) F-actin organization and pollen tube tip growth in Arabidopsis are dependent on the gametophyte-specific Armadillo repeat protein ARO1. Plant Cell 20:2798–2814
16. Fabro G et al (2011) Multiple candidate effectors from the oomycete pathogen Hyaloperonospora arabidopsidis suppress host plant immunity. PLoS Pathog 7:e1002348
17. Braun P, Gingras A-C (2012) History of protein- protein interactions: From egg-white to complex networks. Proteomics 12: 1478–1498
18. Field J et al (1988) Purification of a RASresponsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell Biol 8:2159–2165
19. Evan GI et al (1985) Isolation of monoclonal antibodies specific for human c-myc protooncogene product. Mol Cell Biol 5: 3610–3616
20. Brizzard B (2008) Epitope tagging. Biotechniques 44:693–695
21. Nakagawa T et al (2007) Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation. J Biosci Bioeng 104:34–41
22. Earley KW et al (2006) Gateway-compatible vectors for plant functional genomics and proteomics. Plant J 45:616–629
23. Caillaud M-C et al (2012) Subcellular localization of the Hpa RxLR effector repertoire identify es a tonoplast-associated protein HaRxL17 that confers enhanced plant susceptibility. Plant J 69:252–265
24. Nelson BK, Cai X, Nebenführ A (2007) A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants. Plant J 51:1126–1136
25. Rangan L, Vogel C, Srivastava A (2008) Analysis of context sequence surrounding translation initiation site from complete genome of model plants. Mol Biotechnol 39:207–213
26. Weigel D, Glazebrook J (2006) Transformation of Agrobacterium using the freeze-thaw method. CSH Protoc 2006
27. Latijnhouwers M et al (2005) An Arabidopsis GRIP domain protein locates to the trans- Golgi and binds the small GTPase ARL1. Plant J 44:459–470
28. Boevink PC, Birch PRJ, Whisson SC (2011) Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue. Methods Mol Biol 712:195–209
29. Voinnet O et al (2003) An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J 33:949–956
30. Win J, Kamoun S, Jones AME (2011) Purification of effector-target protein complexes via transient expression in Nicotiana benthamiana. Methods Mol Biol 712:181–194
31. Zeiser E et al (2011) MosSCI and gateway compatible plasmid toolkit for constitutive and inducible expression of transgenes in the C. Elegans germline. PLoS ONE 6:e20082
32. Villefranc JA, Amigo J, Lawson ND (2007) Gateway compatible vectors for analysis of gene function in the zebrafish. Dev Dyn 236:3077–3087
33. Karimi M, Inzé D, Depicker A (2002) GATEWAY vectors for Agrobacterium - mediated plant transformation. Trends Plant Sci 7:193–195
34. Nakagawa T et al (2007) Improved Gateway binary vectors: High-performance vectors for creation of fusion constructs in transgenic analysis of plants. Biosci Biotechnol Biochem 71:2095–2100