MosSCI and gateway compatible plasmid toolkit for constitutive and inducible expression of transgenes in the c. elegans germline

PLoS ONE

Volumn 6, Issue 5, 2011, Pages

  Zeiser, Eva ^a   Frøkjær Jensen, Christian ^b,c   Jorgensen, Erik ^c   Ahringer, Julie ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3' UNTRANSLATED REGION; AMINO TERMINAL SEQUENCE; ARTICLE; CAENORHABDITIS ELEGANS; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; EMBRYO DEVELOPMENT; EXPRESSION VECTOR; GENE EXPRESSION; GENETIC RECOMBINATION; INTRON; MEX 5 GENE; NONHUMAN; PLASMID; PROMOTER REGION; TBB 2 GENE; TRANSGENE; VIRUS CELL TRANSFORMATION; ANIMAL; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS; GERM CELL; HEAT SHOCK RESPONSE; METABOLISM; TRANSGENIC ANIMAL;

CAENORHABDITIS ELEGANS;

CAENORHABDITIS ELEGANS PROTEIN; MEX 5 PROTEIN, C ELEGANS; MEX-5 PROTEIN, C ELEGANS;

3' UNTRANSLATED REGIONS; ANIMALS; ANIMALS, GENETICALLY MODIFIED; CAENORHABDITIS ELEGANS; CAENORHABDITIS ELEGANS PROTEINS; GENE EXPRESSION REGULATION; GENETIC VECTORS; GERM CELLS; HEAT-SHOCK RESPONSE; PLASMIDS; PROMOTER REGIONS, GENETIC; TRANSGENES;

EID: 79957554781     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0020082     Document Type: Article

References (15)

1. Kelly WG, Xu S, Montgomery MK, Fire A, (1997) Distinct requirements for somatic and germline expression of a generally expressed Caernorhabditis elegans gene. Genetics 146: 227-238.
2. Praitis V, Casey E, Collar D, Austin J, (2001) Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics 157: 1217-1226.
3. Frøkjær-Jensen C, Davis MW, Hopkins CE, Newman BJ, Thummel JM, et al. (2008) Single-copy insertion of transgenes in Caenorhabditis elegans. Nat Genet 40: 1375-1383.
4. Robert V, Bessereau JL, (2007) Targeted engineering of the Caenorhabditis elegans genome following Mos1-triggered chromosomal breaks. EMBO J 26: 170-183.
5. Bazopoulou D, Tavernarakis N, (2009) The NemaGENETAG initiative: large scale transposon insertion gene-tagging in Caenorhabditis elegans. Genetica 137: 39-46.
6. Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, et al. (2010) An antibiotic selection marker for nematode transgenesis. Nat Methods 7: 721-723.
7. Dupuy D, Li QR, Deplancke B, Boxem M, Hao T, et al. (2004) A first version of the Caenorhabditis elegans Promoterome. Genome Res 14: 2169-2175.
8. Reboul J, Vaglio P, Rual JF, Lamesch P, Martinez M, et al. (2003) C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression. Nat Genet 34: 35-41.
9. Mangone M, Manoharan AP, Thierry-Mieg D, Thierry-Mieg J, Han T, et al. (2010) The landscape of C. elegans 3′UTRs. Science 329: 432-435.
10. Merritt C, Rasoloson D, Ko D, Seydoux G, (2008) 3′ UTRs are the primary regulators of gene expression in the C. elegans germline. Curr Biol 18: 1476-1482.
11. Okkema PG, Harrison SW, Plunger V, Aryana A, Fire A, (1993) Sequence requirements for myosin gene expression and regulation in Caenorhabditis elegans. Genetics 135: 385-404.
12. Stringham EG, Dixon DK, Jones D, Candido EP, (1992) Temporal and spatial expression patterns of the small heat shock (hsp16) genes in transgenic Caenorhabditis elegans. Mol Biol Cell 3: 221-233.
13. Tursun B, Patel T, Kratsios P, Hobert O, (2011) Direct conversion of C. elegans germ cells into specific neuron types. Science 331: 304-308.
14. Fire A, Ahnn J, Kelly W, Harfe B, Kostas S, et al. (1998) GFP applications in C. elegans. In: Chalfie M, Kain S, editors. GFP Strategies and Applications pp. 153-168.
15. Andrews R, Ahringer J, (2007) Asymmetry of early endosome distribution in C. elegans embryos. PLoS One 2: e493.