Comparison of two multiplex PCR assays for the detection of respiratory viral infections

Clinical Respiratory Journal

Volumn 8, Issue 4, 2014, Pages 391-396

  Kim, Hanah ^a   Hur, Mina ^a   Moon, Hee Won ^a   Yun, Yeo Min ^a   Cho, Hyun Chan ^b  

^a Konkuk University School of Medicine   (South Korea)

^b Hallym University College of Medicine   (South Korea)

Author keywords

Multiplex PCR; One step RV; Performance; Respiratory virus; Seeplex RV

Indexed keywords

ADOLESCENT; ADULT; AGED; ARTICLE; CHILD; CLINICAL LABORATORY; COMPARATIVE STUDY; DIAGNOSTIC PROCEDURE; DIAGNOSTIC TEST ACCURACY STUDY; FEMALE; HUMAN; LUNG LAVAGE; MAJOR CLINICAL STUDY; MALE; MULTIPLEX POLYMERASE CHAIN REACTION; NOSE SMEAR; PATHOGENESIS; PRIORITY JOURNAL; RESPIRATORY VIRUS; SPUTUM ANALYSIS; THROAT CULTURE; VERY ELDERLY; VIRAL RESPIRATORY TRACT INFECTION; VIRUS DETECTION; COHORT ANALYSIS; INFANT; MIDDLE AGED; NEWBORN; PRESCHOOL CHILD; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY TRACT INFECTIONS; SENSITIVITY AND SPECIFICITY; SOUTH KOREA; VIROLOGY; VIRUS DISEASES; YOUNG ADULT;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; CHILD; CHILD, PRESCHOOL; COHORT STUDIES; FEMALE; HUMANS; INFANT; INFANT, NEWBORN; MALE; MIDDLE AGED; MULTIPLEX POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; REPUBLIC OF KOREA; RESPIRATORY TRACT INFECTIONS; SENSITIVITY AND SPECIFICITY; VIRUS DISEASES; YOUNG ADULT;

EID: 84908376615     PISSN: 17526981     EISSN: 1752699X     Source Type: Journal    
DOI: 10.1111/crj.12083     Document Type: Article

References (25)

1. Chretien J, Holland W, Macklem P, Murray J, Woolcock A. Acute respiratory infections in children. A global public-health problem. N Engl J Med. 1984;310: 982-984.
2. Hall CB, Weinberg GA, Iwane MK, etal. The burden of respiratory syncytial virus infection in young children. N Engl J Med. 2009;360: 588-598.
3. Pierce VM, Elkan M, Leet M, McGowan KL, Hodinka RL. Comparison of the Idaho technology FilmArray system to real-time PCR for detection of respiratory pathogens in children. J Clin Microbiol. 2012;50: 364-371.
4. Sanghavi SK, Bullotta A, Husain S, Rinaldo CR. Clinical evaluation of multiplex real-time PCR panels for rapid detection of respiratory viral infections. J Med Virol. 2012;84: 162-169.
5. Templeton KE, Scheltinga SA, Beersma MF, Kroes AC, Claas EC. Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza a and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4. J Clin Microbiol. 2004;42: 1564-1569.
6. Mahony JB, Petrich A, Smieja M. Molecular diagnosis of respiratory virus infections. Crit Rev Clin Lab Sci. 2011;48: 217-249.
7. Mahony JB, Blackhouse G, Babwah J, etal. Cost analysis of multiplex PCR testing for diagnosing respiratory virus infections. J Clin Microbiol. 2009;47: 2812-2817.
8. Yang JH, Huang PY, Shie SS, Huang CG, Tsao KC, Huang CT. Diagnostic capacity of rapid influenza antigen test: reappraisal with experience from the 2009 H1N1 pandemic. J Microbiol Immunol Infect. 2012;45: 102-107.
9. Casiano-Colon AE, Hulbert BB, Mayer TK, Walsh EE, Falsey AR. Lack of sensitivity of rapid antigen tests for the diagnosis of respiratory syncytial virus infection in adults. J Clin Virol. 2003;28: 169-174.
10. Espy MJ, Uhl JR, Sloan LM, etal. Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin Microbiol Rev. 2006;19: 165-256.
11. Speers DJ. Clinical applications of molecular biology for infectious diseases. Clin Biochem Rev. 2006;27: 39-51.
12. Gharabaghi F, Hawan A, Drews SJ, Richardson SE. Evaluation of multiple commercial molecular and conventional diagnostic assays for the detection of respiratory viruses in children. Clin Microbiol Infect. 2011;17: 1900-1906.
13. Liolios L, Jenney A, Spelman D, Kotsimbos T, Catton M, Wesselingh S. Comparison of a multiplex reverse transcription-PCR-enzyme hybridization assay with conventional viral culture and immunofluorescence techniques for the detection of seven viral respiratory pathogens. J Clin Microbiol. 2001;39: 2779-2783.
14. Lee JH, Chun JK, Kim DS, Park Y, Choi JR, Kim HS. Identification of adenovirus, influenza virus, parainfluenza virus, and respiratory syncytial virus by two kinds of multiplex polymerase chain reaction (PCR) and a shell vial culture in pediatric patients with viral pneumonia. Yonsei Med J. 2010;51: 761-767.
15. Drews SJ, Blair J, Lombos E, DeLima C, Burton L, Mazzulli T, Low DE. Use of the Seeplex RV Detection kit for surveillance of respiratory viral outbreaks in Toronto, Ontario, Canada. Ann Clin Lab Sci. 2008;38: 376-379.
16. Cho CH, Chulten B, Lee CK, Nam MH, Yoon SY, Lim CS, Cho Y, Kim YK. Evaluation of a novel real-time RT-PCR using TOCE technology compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses. J Clin Virol. 2013;57: 338-342.
17. Kim HK, Oh SH, Yun KA, Sung H, Kim MN. Comparison of Anyplex II RV16 with the xTAG respiratory viral panel and Seeplex RV15 for detection of respiratory viruses. J Clin Microbiol. 2013;51: 1137-1141.
18. Cho GS, Moon BJ, Lee BJ, Gong CH, Kim NH, Kim YS, Kim HS, Jang YJ. High rates of detection of respiratory viruses in the nasal washes and mucosae of patients with chronic rhinosinusitis. J Clin Microbiol. 2013;51: 979-984.
19. Pillet S, Lardeux M, Dina J, Grattard F, Verhoeven P, Le Goff J, Vabret A, Pozzetto B. Comparative evaluation of six commercialized multiplex PCR kits for the diagnosis of respiratory infections. PLoS ONE. 2013;8: e72174.
20. Deyde VM, Xu X, Bright RA, Shaw M, Smith CB, Zhang Y, Shu Y, Gubareva LV, Cox NJ, Klimov AI. Surveillance of resistance to adamantanes among influenza A(H3N2) and A(H1N1) viruses isolated worldwide. J Infect Dis. 2007;196: 249-257.
21. Gubareva LV, Kaiser L, Hayden FG. Influenza virus neuraminidase inhibitors. Lancet. 2000;355: 827-835.
22. Cooper NJ, Sutton AJ, Abrams KR, Wailoo A, Turner D, Nicholson KG. Effectiveness of neuraminidase inhibitors in treatment and prevention of influenza A and B: systematic review and meta-analyses of randomised controlled trials. BMJ. 2003;326: 1235-1241.
23. Kehl SC, Henrickson KJ, Hua W, Fan J. Evaluation of the Hexaplex assay for detection of respiratory viruses in children. J Clin Microbiol. 2001;39: 1696-1701.
24. Weinberg GA, Erdman DD, Edwards KM, Hall CB, Walker FJ, Griffin MR, Schwartz B. New Vaccine Surveillance Network Study Group. Superiority of reverse-transcription polymerase chain reaction to conventional viral culture in the diagnosis of acute respiratory tract infections in children. J Infect Dis. 2004;189: 706-710.
25. van den Hoogen BG, Osterhaus DM, Fouchier RA. Clinical impact and diagnosis of human metapneumovirus infection. Pediatr Infect Dis J. 2004;23: S25-32.