Versatile on-demand droplet generation for controlled encapsulation

Biomicrofluidics

Volumn 8, Issue 3, 2014, Pages

  Rhee, Minsoung ^a   Liu, Peng ^a,c   Meagher, Robert J ^a   Light, Yooli K ^a   Singh, Anup K ^a,b  

^a SANDIA NATIONAL LABORATORIES   (United States)

^b JOINT BIOENERGY INSTITUTE   (United States)

^c TSINGHUA UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

DATA ENCAPSULATION; ESCHERICHIA COLI; PARALLEL FLOW; PUMPS;

CONSTANT PRESSURES; DROPLET GENERATION; DROPLET-BASED MICROFLUIDICS; ESCHERICHIA COLI CELLS; OPTIMIZED CONDITIONS; RESISTANCE BRIDGES; SELECTIVE ENCAPSULATION; SUBSEQUENT DATA ANALYSIS;

DROPS;

ESCHERICHIA COLI;

EID: 84908308619     PISSN: 19321058     EISSN: None     Source Type: Journal    
DOI: 10.1063/1.4874715     Document Type: Article

References (28)

1. S. Y. Teh, R. Lin, L. H. Hung, and A. P. Lee, "Droplet microfluidics," Lab Chip 8(2), 198-220 (2008).
2. A. Huebner, S. Sharma, M. Srisa-Art, F. Hollfelder, J. B. Edel, and A. J. Demello, "Microdroplets: A sea of applications?" Lab Chip 8(8), 1244-1254 (2008).
3. R. R. Pompano, W. Liu, W. Du, and R. F. Ismagilov, "Microfluidics using spatially defined arrays of droplets in one, two, and three dimensions," Annu. Rev. Anal. Chem. 4, 59-81 (2011).
4. M. Rhee and M. A. Burns, "Drop mixing in a microchannel for lab-on-a-chip platforms," Langmuir 24(2), 590-601 (2008).
5. M. T. Guo, A. Rotem, J. A. Heyman, and D. A. Weitz, "Droplet microfluidics for high-throughput biological assays," Lab Chip 12(12), 2146-2155 (2012).
6. M. G. Simon, R. Lin, J. S. Fisher, and A. P. Lee, "A Laplace pressure based microfluidic trap for passive droplet trapping and controlled release," Biomicrofluidics 6(1), 014110 (2012).
7. E. Brouzes, M. Medkova, N. Savenelli, D. Marran, M. Twardowski, J. B. Hutchison, J. M. Rothberg, D. R. Link, N. Perrimon, and M. L. Samuels, "Droplet microfluidic technology for single-cell high-throughput screening," Proc. Natl. Acad. Sci. U.S.A. 106(34), 14195-14200 (2009).
8. W. Li, H. H. Pham, Z. Nie, B. MacDonald, A. Guenther, and E. Kumacheva, "Multi-step microfluidic polymerization reactions conducted in droplets: the internal trigger approach," J. Am. Chem. Soc. 130(30), 9935-9941 (2008).
9. D. J. Eastburn, A. Sciambi, and A. R. Abate, "Picoinjection enables digital detection of RNA with droplet RT-PCR," PLoS One 8(4), e62961 (2013).
10. B. Zheng, J. D. Tice, and R. F. Ismagilov, "Formation of arrayed droplets of soft lithography and two-phase fluid flow, and application in protein crystallization," Adv. Mater. 16(15), 1365-1368 (2004).
11. I. Shestopalov, J. D. Tice, and R. F. Ismagilov, "Multi-step synthesis of nanoparticles performed on millisecond time scale in a microfluidic droplet-based system," Lab Chip 4(4), 316-321 (2004).
12. C. Chang, J. Sustarich, R. Bharadwaj, A. Chandrasekaran, P. D. Adams, and A. K. Singh, "Droplet-based microfluidic platform for heterogeneous enzymatic assays," Lab Chip 13(9), 1817-1822 (2013).
13. T. Thorsen, R. W. Roberts, F. H. Arnold, and S. R. Quake, "Dynamic pattern formation in a vesicle-generating microfluidic device," Phys. Rev. Lett. 86(18), 4163-4166 (2001).
14. T. D. Perroud, R. J. Meagher, M. P. Kanouff, R. F. Renzi, M. Wu, A. K. Singh, and K. D. Patel, "Isotropically etched radial micropore for cell concentration, immobilization, and picodroplet generation," Lab Chip 9(4), 507-515 (2009).
15. S. L. Anna, N. Bontoux, and H. A. Stone, "Formation of dispersions using 'flow focusing' in microchannels," Appl. Phys. Lett. 82(3), 364-366 (2003).
16. P. Guillot, A. Colin, A. S. Utada, and A. Ajdari, "Stability of a jet in confined pressure-driven biphasic flows at low Reynolds numbers," Phys. Rev. Lett. 99(10), 104502 (2007).
17. A. Gunther and K. F. Jensen, "Multiphase microfluidics: From flow characteristics to chemical and materials synthesis," Lab Chip 6(12), 1487-1503 (2006).
18. C. N. Baroud, F. Gallaire, and R. Dangla, "Dynamics of microfluidic droplets," Lab Chip 10(16), 2032-2045 (2010).
19. R. Seemann, M. Brinkmann, T. Pfohl, and S. Herminghaus, "Droplet based microfluidics," Rep. Prog. Phys. 75(1), 016601 (2012).
20. E. Fradet, C. McDougall, P. Abbyad, R. Dangla, D. McGloin, and C. N. Baroud, "Combining rails and anchors with laser forcing for selective manipulation within 2D droplet arrays," Lab Chip 11(24), 4228-4234 (2011).
21. S. J. Zeng, B. W. Li, X. O. Su, J. H. Qin, and B. C. Lin, "Microvalve-actuated precise control of individual droplets in microfluidic devices," Lab Chip 9(10), 1340-1343 (2009).
22. H. Zec, T. D. Rane, and T. H. Wang, "Microfluidic platform for on-demand generation of spatially indexed combinatorial droplets," Lab Chip 12(17), 3055-3062 (2012).
23. R. M. Lorenz, J. S. Edgar, G. D. M. Jeffries, and D. T. Chiu, "Microfluidic and optical systems for the on-demand generation and manipulation of single femtoliter-volume aqueous droplets," Anal. Chem. 78(18), 6433-6439 (2006).
24. J. Xu and D. Attinger, "Drop on demand in a microfluidic chip," J. Micromech. Microeng. 18(6), 065020 (2008).
25. G. M. Whitesides, E. Ostuni, S. Takayama, X. Y. Jiang, and D. E. Ingber, "Soft lithography in biology and biochemistry," Annu. Rev. Biomed. Eng. 3, 335-373 (2001).
26. L. Mazutis and A. D. Griffiths, "Selective droplet coalescence using microfluidic systems," Lab Chip 12(10), 1800-1806 (2012).
27. P. Garstecki, M. J. Fuerstman, H. A. Stone, and G. M. Whitesides, "Formation of droplets and bubbles in a microfluidic T-junction-Scaling and mechanism of break-up," Lab Chip 6(3), 437-446 (2006).
28. supplementary material at http://dx.doi.org/10.1063/1.4874715 for more detailed image sequences of multiple droplet generation and for strategies to prevent the pumping fluid from entering on-demand generated droplets.