Isolation of human monocytes by double gradient centrifugation and their differentiation to macrophages in Teflon-coated cell culture bags

Journal of Visualized Experiments

Volumn , Issue 91, 2014, Pages

  Menck, Kerstin ^a   Behme, Daniel ^a   Pantke, Mathias ^a   Reiling, Norbert ^b   Binder, Claudia ^a   Pukrop, Tobias ^a   Klemm, Florian ^a  

^a UNIVERSITY MEDICAL CENTER GÖTTINGEN   (Germany)

^b Research Center Borstel   (Germany)

Author keywords

Density gradient; Differentiation; Immunology; Isolation; Issue 91; Macrophages; Monocytes; PBMCs; Teflon coated cell culture bags

Indexed keywords

POLITEF;

CELL DIFFERENTIATION; CHEMISTRY; CULTURE TECHNIQUE; CYTOLOGY; DENSITY GRADIENT CENTRIFUGATION; DEVICES; HUMAN; MACROPHAGE; MONOCYTE; PHYSIOLOGY; PROCEDURES;

CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CENTRIFUGATION, DENSITY GRADIENT; HUMANS; MACROPHAGES; MONOCYTES; POLYTETRAFLUOROETHYLENE;

EID: 84908300568     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51554     Document Type: Article

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