Isolation of healthy individuals' and rheumatoid arthritis patients' peripheral blood neutrophils by the gelatin and Ficoll-Hypaque methods: Comparative efficiency and impact on the neutrophil oxidative metabolism and Fcγ receptor expression

Journal of Immunological Methods

Volumn 412, Issue , 2014, Pages 70-77

  Paoliello Paschoalato, A B ^a   Azzolini, A E C S ^a   Cruz, M F C ^a   Marchi, L F ^a   Kabeya, L M ^a   Donadi, E A ^a   Lucisano Valim, Y M ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Fc gamma receptor; Ficoll Hypaque; Gelatin; Neutrophils; Oxidative metabolism; Rheumatoid arthritis

Indexed keywords

CD16 ANTIGEN; CD32 ANTIGEN; CD64 ANTIGEN; DIATRIZOATE; FC RECEPTOR; FICOLL; FORMYLMETHIONYLLEUCYLPHENYLALANINE; GELATIN; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; REACTIVE OXYGEN METABOLITE; ANTIGEN ANTIBODY COMPLEX;

ADULT; AEROBIC METABOLISM; ANTIGEN ANTIBODY COMPLEX; ARTICLE; CELL ISOLATION; CELL POPULATION; CLINICAL ARTICLE; COMPARATIVE EFFECTIVENESS; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; FEMALE; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; LEUKOCYTE METABOLISM; LYMPHOCYTE; MONOCYTE; NEUTROPHIL; PERIPHERAL BLOOD NEUTROPHIL; PROTEIN EXPRESSION; RHEUMATOID ARTHRITIS; ARTHRITIS, RHEUMATOID; CELL COUNT; CELL SEPARATION; CELL SURVIVAL; COMPARATIVE STUDY; GENETICS; IMMUNOLOGY; LEUKOCYTE ACTIVATION; METABOLISM; OXIDATIVE STRESS; PATHOLOGY; PROCEDURES;

ANTIGEN-ANTIBODY COMPLEX; ARTHRITIS, RHEUMATOID; CELL COUNT; CELL SEPARATION; CELL SURVIVAL; FICOLL; GELATIN; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; HUMANS; N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE; NEUTROPHIL ACTIVATION; NEUTROPHILS; OXIDATIVE STRESS; RECEPTORS, IGG;

EID: 84908269562     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2014.07.001     Document Type: Article

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