Respiratory burst activity of polymorphonuclear cells is dependent on the cell preparation technique

Acta Anaesthesiologica Scandinavica

Volumn 47, Issue 6, 2003, Pages 702-706

  Zhao, Jing ^a   Juettner, B ^b   Scheinichen, D ^b   Jaeger, K ^b   Heine, J ^b   Piepenbrock, S ^b  

^a PEKING UNION MEDICAL COLLEGE HOSPITAL   (China)

^b NONE

Author keywords

Cell preparation; Flow cytometry; Neutrophils; Propofol; Respiratory burst

Indexed keywords

ANESTHETIC AGENT; BACTERIAL TOXIN; FORMYLMETHIONYLLEUCYLPHENYLALANINE; PHORBOL 13 ACETATE 12 MYRISTATE; PROPOFOL; SUPEROXIDE;

ARTICLE; BLOOD CHEMISTRY; CELL ACTIVITY; CELL FRACTIONATION; CELL ISOLATION; CELL SUSPENSION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG EFFECT; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; IN VITRO STUDY; INTERMETHOD COMPARISON; NORMAL HUMAN; POLYMORPHONUCLEAR CELL; PRIORITY JOURNAL; RESPIRATORY BURST;

ANESTHETICS, INTRAVENOUS; BLOOD SEDIMENTATION; CHEMOTAXIS, LEUKOCYTE; CYTOLOGICAL TECHNIQUES; ESCHERICHIA COLI; FICOLL; HUMANS; N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE; NEUTROPHILS; PHAGOCYTOSIS; PROPOFOL; RESPIRATORY BURST; SUPEROXIDES; TETRADECANOYLPHORBOL ACETATE;

EID: 0037869055     PISSN: 00015172     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-6576.2003.00121.x     Document Type: Article

References (12)

1. Stevenson GW, Hall SC, Rudnick S, Seleny FL. The effect of anesthetic agents on the human immune response. Anesthesiology 1990: 72: 542-52.
2. Heine J, Leuwer M, Scheinichen D et al. Flow cytometry evaluation of the in vitro influence of four iv anaesthetics on respiratory burst of neutrophils. Br J Anaesth 1996: 77: 387-92.
3. Weiss M, Birkhahn A, Krone M, Schneider EM. Do etomidate and propofol influence oxygen radical production of neutrophils? Immunopharmacol Immunotoxicol 1996: 18: 291-307.
4. Gelb AW, Lok P. Etomidate reversibly depresses human neutrophil chemiluminescence. Anesthesiology 1987: 66: 60-3.
5. Mikawa K, Akamatsu H, Nishina K et al. Propofol inhibits human neutrophil functions. Anesthes Analg 1998: 87: 695-701.
6. Davidson JAH, Boom SJ, Pearsall FJ et al. Comparison of the effects of four i.v. anaesthetic agents on polymorphonuclear leukocyte function. Br J Anaesth 1995: 74: 315-8.
7. Böyum A. Isolation of mononuclear cells and granulocytes from human blood: isolation of mononuclear cells by combining centrifugation and sedimentation at 1 G. Scand J Clin Laboratory Invest 1968: 21 (Suppl. 97): 77-89.
8. Seligmann BE, Gallin JI. Use of Lipophilic probes of membrane potential to assess human neutrophil activation. Abnormality in chronic granumatous disease. J Clin Invest 1980: 66: 493-503.
9. Heller A, Heller S, Blecken S. Effects of intravenous anesthetics on bacterial elimination in human blood in vitro. Acta Anaesthesiol Scand 1998: 42: 518-26.
10. Watson F, Robinson JJ, Edwards SW. Neutrophil function in whole blood and after purification: changes in receptor expression, oxidase activity and responsiveness to cytokines. Biosci Rep 1992: 12: 123-33.
11. Mandel G, Clark W. Functional properties of EL-4 tumor cells with lipid altered membrahes. J Immunol 1978: 120: 1937-43.
12. Heine J, Jaeger K, Osthaus A. Anesthesia with propofol decreases fMLP-induced neutrophil respiratory burst but not phagocytosis compared with isoflurane. Br J Anaesth 2000: 85: 424-30.