Novel biomimetic tripolymer scaffolds consisting of chitosan, collagen type 1, and hyaluronic acid for bone marrow-derived human mesenchymal stem cells-based bone tissue engineering

Journal of Biomedical Materials Research - Part B Applied Biomaterials

Volumn 102, Issue 8, 2014, Pages 1825-1834

  Mathews, Smitha ^a   Bhonde, Ramesh ^b   Gupta, Pawan Kumar ^b   Totey, Satish ^c  

^a CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY   (India)

^b MANIPAL UNIVERSITY   (India)

^c DIL Complex   (India)

Author keywords

Biomimetic scaffold; Bone tissue engineering; Chitosan; Mesenchymal stem cells; Osteoblast differentiation

Indexed keywords

BIOMIMETICS; BONE; CELL ADHESION; CELL CULTURE; CELL ENGINEERING; CELL PROLIFERATION; CHITOSAN; COLLAGEN; FUNCTIONAL POLYMERS; HYALURONIC ACID; ORGANIC ACIDS; PHOSPHATASES; SCANNING ELECTRON MICROSCOPY; STEM CELLS; TISSUE; TISSUE REGENERATION;

BIOMIMETIC SCAFFOLDS; BONE TISSUE ENGINEERING; EXTRACELLULAR MATRICES; HUMAN BONE MARROW DERIVED MESENCHYMAL STEM CELLS; HUMAN MESENCHYMAL STEM CELLS; IMMUNOFLUORESCENT STAINING; MESENCHYMAL STEM CELL; OSTEOBLAST DIFFERENTIATION;

SCAFFOLDS (BIOLOGY);

ALKALINE PHOSPHATASE; BIOMIMETIC MATERIAL; CHITOSAN DERIVATIVE; COLLAGEN TYPE 1; HYALURONIC ACID DERIVATIVE; SCLEROPROTEIN; CHITOSAN; HYALURONIC ACID;

ADULT; ARTICLE; BIOCOMPATIBILITY; BIOMIMETICS; BONE DEVELOPMENT; BONE MARROW DERIVED MESENCHYMAL STEM CELL; BONE REGENERATION; BONE TISSUE; CELL ADHESION; CELL DIFFERENTIATION; CELL MIGRATION; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE TEST; OSTEOBLAST; SCANNING ELECTRON MICROSCOPY; TISSUE ENGINEERING; TISSUE SCAFFOLD; BONE MARROW CELL; CELL CULTURE; CELL MOTION; CELL SURVIVAL; CHEMISTRY; CYTOLOGY; FEMALE; MALE; MESENCHYMAL STROMA CELL; METABOLISM;

ADULT; BIOMIMETIC MATERIALS; BONE MARROW CELLS; BONE REGENERATION; CELL DIFFERENTIATION; CELL MOVEMENT; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; CHITOSAN; COLLAGEN TYPE I; FEMALE; HUMANS; HYALURONIC ACID; MALE; MESENCHYMAL STROMAL CELLS; OSTEOGENESIS; TISSUE ENGINEERING; TISSUE SCAFFOLDS;

EID: 84908118025     PISSN: 15524973     EISSN: 15524981     Source Type: Journal    
DOI: 10.1002/jbm.b.33152     Document Type: Article

References (22)

1. Daher RJ, Chahine NO, Greenberg AS, Sgaglione NA, Grande DA. New methods to diagnose and treat cartilage degeneration. Nat Rev Rheumatol 2009;5:599-607.
2. Hunt JA. Regenerative medicine: Materials in a cellular world. Nat Mater 2008;7:617-618.
3. Chai C, Leong KW. Biomaterials approach to expand and direct differentiation of stem cells. Mol Ther 2007;15:467-480.
4. Ma PX. Biomimetic materials for tissue engineering. Adv Drug Deliv Rev 2008;60:184-198.
5. Ode A, Duda GN, Glaeser JD, Matziolis G, Frauenschuh S, Perka C, Wilson CJ, Kasper G. Toward biomimetic materials in bone regeneration: Functional behavior of mesenchymal stem cells on a broad spectrum of extracellular matrix components. J Biomed Mater Res A 2010;95:1114-1124.
6. Grayson WL, Martens TP, Eng GM, Radisic M, Vunjak-Novakovic G. Biomimetic approach to tissue engineering. Semin Cell Dev Biol 2009;20:665-673.
7. Gentile P, Mattioli-Belmonte M, Chiono V, Ferretti C, Baino F, Tonda-Turo C, Vitale-Brovarone C, Pashkuleva I, Reis RL, Ciardelli G. Bioactive glass/polymer composite scaffolds mimicking bone tissue. J Biomed Mater Res A 2012;100:2654-2667.
8. Mathews S, Bhonde R, Gupta PK, Totey S. A novel tripolymer coating demonstrating the synergistic effect of chitosan, collagen type 1 and hyaluronic acid on osteogenic differentiation of human bone marrow derived mesenchymal stem cells. Biochem Biophys Res Commun 2011;414:270-276.
9. Lin YC, Tan FJ, Marra KG, Jan SS, Liu DC. Synthesis and characterization of collagen/hyaluronan/chitosan composite sponges for potential biomedical applications. Acta Biomater 2099;5:2591-2600.
10. Pal R, Hanwate M, Jan M, Totey S. Phenotypic and functional comparison of optimum culture conditions for upscaling of bone marrow-derived mesenchymal stem cells. J Tissue Eng Regen Med 2009;3:163-174.
11. Tae S-K, Lee S-H, Park J-S, Im G-I. Mesenchymal stem cells for tissue engineering and regenerative medicine. Biomed Mater 2006;1:63-71.
12. Mathews S, Bhonde R, Gupta PK, Totey S. Extracellular matrix protein mediated regulation of the osteoblast differentiation of bone marrow derived human mesenchymal stem cells. Differentiation 2012;84:185-192.
13. Mathews S, Mathew SA, Bhonde R, Gupta PK, Totey S. Glycosaminoglycans enhanced the osteoblast differentiation of bone marrow derived human mesenchymal stem cells. J Tissue Eng Regen Med 2014;8:143-152.
14. Mathews S, Gupta PK, Bhonde R, Totey S. Chitosan enhances mineralization during osteoblast differentiation of human bone marrow-derived mesenchymal stem cells, by up-regulating the associated genes. Cell Prolif 2011;44:537-549.
15. Yang H, Zhang Q. Preparation and characterization of collagen-GAGs bioactive matrices for tissue engineering. J Mater Sci Technol 2001;17:495-500.
16. Shanmugasundaram N, Ravichandran P, Reddy PN, Ramamurty N, Pal S, Rao KP. Collagen-chitosan polymeric scaffolds for the in vitro culture of human epidermoid carcinoma cells. Biomaterials 2001;22:1943-1951.
17. Correia CR, Moreira-Teixeira LS, Moroni L, Reis RL, van Blitterswijk CA, Karperien M, Mano JF. Chitosan scaffolds containing hyaluronic acid for cartilage tissue engineering. Tissue Eng Part C Methods 2011;17:717-730.
18. Ravindran S, Gao Q, Kotecha M, Magin RL, Karol S, Bedran-Russo A, George A. Biomimetic extracellular matrix-incorporated scaffold induces osteogenic gene expression in human marrow stromal cells. Tissue Eng Part A 2012;18:295-309.
19. Globus RK, Doty SB, Lull JC, Holmuhamedov E, Humphries MJ, Damsky CH. Fibronectin is a survival factor for differentiated osteoblasts. J Cell Sci 1998;111(Pt. 10):1385-1393.
20. Moursi AM, Damsky CH, Lull J, Zimmerman D, Doty SB, Aota S, Globus RK. Fibronectin regulates calvarial osteoblast differentiation. J Cell Sci 1996;109(Pt. 6):1369-1380.
21. Shah AK, Lazatin J, Sinha RK, Lennox T, Hickok NJ, Tuan RS. Mechanism of BMP-2 stimulated adhesion of osteoblastic cells to titanium alloy. Biol Cell 1999;91:131-142.
22. Prasanna SJ, Gopalakrishnan D, Shankar SR, Vasandan AB. Pro-inflammatory cytokines, IFNgamma and TNFalpha, influence immune properties of human bone marrow and Wharton jelly mesenchymal stem cells differentially. PLoS One 2010;5: e9016.