Cultivation of human bone-like tissue from pluripotent stem cell-derived osteogenic progenitors in perfusion bioreactors

Methods in Molecular Biology

Volumn 1202, Issue , 2014, Pages 173-184

  De Peppo, Giuseppe Maria ^a   Vunjak Novakovic, Gordana ^b   Marolt, Darja ^a  

^a New York Stem Cell Foundation Laboratory   (United States)

^b Department of Biomedical Engineering Department of Medicine Columbia University ^*  (United States)

Author keywords

Bone scaffolds; Bone tissue development; Cell seeding; Human embryonic stem cells; Human induced pluripotent stem cells; Medium flow rate; Mesenchymal like progenitors; Osteogenic differentiation; Osteogenic medium; Perfusion bioreactor

Indexed keywords

ARTICLE; BIOREACTOR; BONE CONDUCTION; BONE GROWTH; BONE PROSTHESIS; BONE REMODELING; BONE TISSUE; EMBRYONIC STEM CELL; HUMAN; HUMAN CELL; HUMAN TISSUE; MESENCHYMAL STEM CELL; NONHUMAN; OSTEOPROGENITOR CELL; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; TISSUE SCAFFOLD; ANIMAL; BONE; BONE DEVELOPMENT; BOVINE; CELL CULTURE; CYTOLOGY; DEVICES; MESENCHYMAL STROMA CELL; METABOLISM; MOUSE; PERFUSION; PHYSIOLOGY; PROCEDURES;

MEMBRANE ANTIGEN;

ANIMALS; ANTIGENS, SURFACE; BIOREACTORS; BONE AND BONES; CATTLE; CELLS, CULTURED; HUMANS; MESENCHYMAL STROMAL CELLS; MICE; OSTEOGENESIS; PERFUSION; PLURIPOTENT STEM CELLS; TISSUE SCAFFOLDS;

EID: 84907340001     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/7651-2013-52     Document Type: Article

References (17)

1. de Peppo GM, Marolt D (2013) Modulating the biochemical and biophysical culture environment to enhance osteogenic differentiation and maturation of human pluripotent stem cell-derived mesenchymal progenitors. Stem Cell Res Ther 5:106
2. Tandon N, Marolt D, Cimetta E, Vunjak-Novakovic G (2013) Bioreactor engineering of stem cell environments. Biotechnol Adv. 7:1020-1031
3. Marolt D, Knezevic M, Vunjak-Novakovic G (2010) Bone tissue engineering with human stem cells. Stem Cell Res Ther 2:10
4. Tachibana M, Amato P, Sparman M, Gutierrez NM, Tippner-HedgesmR, MaH, KangE, Fulati A, Lee HS, Sritanaudomchai H, Masterson K, Larson J, Eaton D, Sadler-Fredd K, Battaglia D, Lee D, Wu D, Jensen J, Patton P, Gokhale S, Stouffer RL, Wolf D, Mitalipov S (2013) Human embryonic stem cells derived by somatic cell nuclear transfer. Cell 6:1228-1238
5. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 5:861-872
6. Murry CE, Keller G (2008) Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development. Cell 4:661-680
7. Kuznetsov SA, Cherman N, Robey PG (2011) In vivo bone formation by progeny of human embryonic stem cells. Stem Cells Dev 2:269-287
8. Levi B, Hyun JS, Montoro DT, Lo DD, Chan CK, Hu S, Sun N, Lee M, Grova M, Connolly AJ, Wu JC, Gurtner GC, Weissman IL, Wan DC, Longaker MT (2012) In vivo directed differentiation of pluripotent stem cells for skeletal regeneration. Proc Natl Acad Sci USA 50:20379-20384
9. Fröhlich M, Grayson WL, Wan LQ, Marolt D, Drobnic M, Vunjak-Novakovic G (2008) Tissue engineered bone grafts: biological requirements, tissue culture and clinical relevance. Curr Stem Cell Res Ther 4:254-264
10. Grayson WL, Fröhlich M, Yeager K, Bhumiratana S, Chan ME, Cannizzaro C, Wan LQ, Liu XS, Guo XE, Vunjak-Novakovic G (2010) Engineering anatomically shaped human bone grafts. Proc Natl Acad Sci USA 8:3299-3304
11. Grayson WL, Bhumiratana S, Cannizzaro C, Chao PH, Lennon DP, Caplan AI, Vunjak-Novakovic G (2008) Effects of initial seeding density and fluid perfusion rate on formation of tissue-engineered bone. Tissue Eng Part A 11:1809-1820
12. Grayson WL, Marolt D, Bhumiratana S, Fröhlich M, Guo XE, Vunjak-Novakovic G (2011) Optimizing the medium perfusion rate in bone tissue engineering bioreactors. Biotechnol Bioeng 5:1159-1170
13. Marcos-Campos I, Marolt D, Petridis P, Bhumiratana S, Schmidt D, Vunjak-Novakovic G (2012) Bone scaffold architecture modulates the development of mineralized bone matrix by human embryonic stem cells. Biomaterials 33:8329-8342
14. de Peppo GM, Marcos-Campos I, Kahler DJ, Alsalman D, Shang L, Vunjak-Novakovic G, Marolt D (2013) Engineering bone tissue substitutes from human induced pluripotent stem cells. Proc Natl Acad Sci USA 21:8680-8685
15. Marolt D, Campos IM, Bhumiratana S, Koren A, Petridis P, Zhang G, Spitalnik PF, Grayson WL, Vunjak-Novakovic G (2012) Engineering bone tissue from human embryonic stem cells. Proc Natl Acad Sci USA 22:8705-8709
16. de Peppo GM, Sjovall P, Lenneras M, Strehl R, Hyllner J, Thomsen P, Karlsson C (2010) Osteogenic potential of human mesenchymal stem cells and human embryonic stem cellderived mesodermal progenitors: a tissue engineering perspective. Tissue Eng Part A 11:3413-3426
17. de Peppo GM, Svensson S, Lenneras M, Synnergren J, Stenberg J, Strehl R, Hyllner J, Thomsen P, Karlsson C (2010) Human embryonic mesodermal progenitors highly resemble human mesenchymal stem cells and display high potential for tissue engineering applications. Tissue Eng Part A 7:2161-2182