Comparison of eight different agars for the recovery of clinically relevant non-O157 Shiga toxin-producing Escherichia coli from baby spinach, cilantro, alfalfa sprouts and raw milk

Food Microbiology

Volumn 46, Issue , 2015, Pages 280-287

  Kase, Julie A ^a   Maounounen Laasri, Anna ^b   Son, Insook ^a   Lin, Andrew ^a   Hammack, Thomas S ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^b OAK RIDGE INSTITUTE FOR SCIENCE AND EDUCATION   (United States)

Author keywords

Bacteriological agar; Fresh produce; Raw milk; STEC

Indexed keywords

AGAR; CULTURE MEDIUM;

ALFALFA; ANIMAL; BACTERIAL COUNT; BACTERIAL INFECTION; BOVINE; CHEMISTRY; COMPARATIVE STUDY; CORIANDRUM; CULTURE MEDIUM; DEVICES; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MILK; PROCEDURES; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; SPINACH;

AGAR; ANIMALS; BACTERIAL INFECTIONS; CATTLE; COLONY COUNT, MICROBIAL; CORIANDRUM; CULTURE MEDIA; MEDICAGO SATIVA; MILK; SHIGA-TOXIGENIC ESCHERICHIA COLI; SPINACIA OLERACEA;

EID: 84907246442     PISSN: 07400020     EISSN: 10959998     Source Type: Journal    
DOI: 10.1016/j.fm.2014.08.020     Document Type: Article

References (30)

1. Anonymous Media and reagents, appendix 1.06. Microbiology Laboratory Guidebook 2011, U.S. Department of Agriculture, Food Safety Inspection Service, Available at:. http://www.fsis.usda.gov/Science/Microbiological_Lab_Guidebook/index.asp.
2. Beutin L., Zimmermann S., Gleier K. Rapid detection and isolation of shiga-like toxin (Verocytotoxin)- producing Escherichia coli by direct testing of individual enterohemolytic colonies from washed sheep blood Agar plates in the VTEC-RPLA assay. J. Clin. Microbiol. 1996, 34:2812-2814.
3. Beutin L., Geier D., Steinriick H., Zimmermann S., Scheutz F. Prevalence and some properties of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals. J. Clin. Microbiol. 1993, 31:2483-2488.
4. Beutin L., Geier D., Zimmermann S., Karch H. Virulence markers of shiga-like toxin producing Escherichia coli strains originating from healthy domestic animals of different species. J. Clin. Microbiol. 1995, 33:631-635.
5. Bielaszewska M., Mellmann A., Zhang W., Ko¨ck R., Fruth A., Bauwens A., Peters G., Karch H. Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study. Lancet Infect. Dis. 2011, 11:671-676.
6. Biolog, Inc. 2013. (last accessed 20.05.14.). http://www.biolog.com/MFG/CoA/Certificate.Pages/Microbial%2520Identification/Media,%2520Dry%2520Powder/Rainbow%2520O157%2520Agar%2520DPM%252080102/CA80102%2520312231%2520JAN17.pdf.
7. Centers for Disease Control and Prevention Recommendations for diagnosis of Shiga toxin-producing Escherichia coli infections by clinical laboratories. MMWR Morb. Mortal. Wkly. Rep. 2009, 58:1-14.
8. Centers for Disease Control and Prevention OutbreakNet, Foodborne Outbreak Online Database 2011, Available:. http://wwwn.cdc.gov/foodborneoutbreaks/Default.aspx.
9. Cerqueira A.M.F., Guth B.E.C., Joaquim R.M., Andrade J.R.C. High occurrence of Shiga toxin-producing Escherichia coli (STEC) in healthy cattle in Rio de Janeiro State, Brazil. Vet. Microbiol. 1999, 70:111-121.
10. CHROMAgar. 2013. (last accessed 20.05.14.). http://www.chromagar.com/fichiers/1382083000LF_EXT_033_STEC_V3.pdf?PHPSESSID=905b0ddd4be73f1709e9bf358210e5e0.
11. Erickson M.C., Doyle M.P. Food as a vehicle for transmission of Shiga toxin- producing Escherichia coli. J. Food Prot. 2007, 70:2426-2449.
12. Feng P., Weagant S.D., Jinneman K. Diarrheagenic Escherichia coli. Bacteriological Analytical Manual 2011, U.S. Food and Drug Administration, College Park, MD, revision A. http://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/UCM070080, eighth ed. R.I. Merker (Ed.).
13. Hancock D.D., Besser T.E., Kinsel M.L., Tarr P.I., Rice D.H., Paros M.G. The prevalence of Escherichia coli 0157:H7 in dairy and beef cattle in Washington State. Epidemiol. Infect. 1994, 113:199-207.
14. Hirvonen J.J., Siitonen A., Kaukoranta S.-S. Usability and performance of CHROMagar STEC medium in detection of Shiga toxin-producing Escherichia coli strains. J. Clin. Microbiol. 2012, 50:3586-3590.
15. Johnson K.E., Thorpe C.M., Sears C.L. The emerging clinical importance of non-O157 Shiga toxin-producing Escherichia coli. Clin. Infect. Dis. 2006, 43:1587-1595.
16. Kalchayanand N., Arthur T.M., Bosilevac J.M., Wells J., Wheeler T.L. Chromogenic agar medium for detection and isolation of Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 from fresh beef and cattle feces. J. Food Prot. 2013, 76(2):192-199.
17. Kase J.A., Borenstein S., Feng P.C.H. Microbial quality of bagged baby spinach and romaine lettuce - effects of top vs. bottom sampling. J. Food Prot. 2012, 75(1):132-136.
18. Kimura N., Kozaki A., Sasaki T., Komatsubara A. Basic study of Beutin's washed sheep blood agar plate used for selective screening of verocytotoxin-producing/enterohemorrhagic Escherichia coli (VTEC/EHEC). Kansenshogaku Zasshi. 1999, 73:318-327.
19. Lin A., Nguyen L., Lee T., Clotilde L.M., Kase J.A., Son I., Carter J.M., Lauzon C.R. Rapid O serogroup identification of the Ten Most Clinically relevant STECs by Luminex bead based suspension array. J. Microbiol. Methods 2011, 87:105-110.
20. Lin A., Nguyen L., Clotilde L.M., Kase J.A., Son I., Lauzon C.R. Isolation of Shiga toxin-producing Escherichia coli from fresh produce using STEC heart infusion washed blood agar with mitomycin-C. J. Food Prot. 2012, 75:2028-2030. 10.4315/0362-028X.JFP-12-157.
21. Posse´ B., De Zutter L., Heyndrickx M., Herman L. Novel differential and confirmation plating media for Shiga toxin producing Escherichia coli serotypes O26, O103, O111, O145 and sorbitol-positive and -negative O157. FEMS Microbiol. Lett. 2008, 282:124-131.
22. Pradel N., Livrelli V., De Champs C., Palcoux J.B., Reynaud A., Scheutz F., Sirot J., Joly B., Forestier C. Prevalence and characterization of Shiga toxin-producing Escherichia coli isolated from cattle, food, and children during a one-year prospective study in France. J. Clin. Microbiol. 2000, 38:1023-1031.
23. R & F Laboratories Product Brochure 2014, (last accessed 20.05.14.). http://rf-products.net/products/escherichia-coli-o157-h7-chromogenic-plating-medium/.
24. R & F Laboratories Product Brochure 2014, (last accessed 20.05.14.). http://rf-products.net/products/non-o157-stec-chromogenic-plating-medium/.
25. Scallan E., Hoekstra R.M., Angulo F.J., Tauxe R.V., Widdowson M., Roy S.L., Jones J.L., Griffin P.M. Foodborne illness acquired in the United States-major pathogens. Emerg. Infect. Dis. 2011, 17:7-15.
26. Son I., Binet R., Maounounen-Laasri A., Lin A., Hammack T.S., Kase J.A. Detection of five Shiga toxin-producing Escherichia coli genes with multiplex PCR. Food Microbiol. 2014, 40:31-40.
27. Sugiyama K., Inoue K., Sakazaki R. Mitomycin-supplemented washed blood agar for the isolation of Shiga toxin-producing Escherichia coli other than O157:H7. Lett. Appl. Microbiol. 2001, 33:193-195.
28. Tillman G., Wasilenko J.L., Simmons M., Lauze T., Miniocozzi J., Oakley B., Narang N., Fratamico P.M., Cray W. Isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121 and O145 from ground beef using modified rainbow agar and post-immunomagnetic separation acid treatment. J. Food Prot. 2012, 75:1548-1554.
29. Tzchoppe M., Martin A., Beutin L. A rapid procedure for the detection and isolation of enterohaemorrhagic Escherichia coli (EHEC) serogroup O26, O103, O111, O118, O121 and O157 strains and the aggregative EHEC O104:H4 strain from ready-to-eat vegetables. Int. J. Food Microbiol. 2012, 152:19-30.
30. U.S.Department of Agriculture.Food Safety and Inspection Service E. coli 0157:H7 Contamination of Beef Products Sept. 20, 2011, vol. 76. FR 182.