Plasma components and platelet activation are essential for the antimicrobial properties of autologous platelet-rich plasma: An in vitrostudy

PLoS ONE

Volumn 9, Issue 9, 2014, Pages

  Drago, Lorenzo ^a,b   Bortolin, Monica ^b   Vassena, Christian ^b   Romanò, Carlo L ^b   Taschieri, Silvio ^a,b   Del Fabbro, Massimo ^a,b  

^a UNIVERSITY OF MILAN   (Italy)

^b IRCCS ISTITUTO ORTOPEDICO GALEAZZI   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBACTERIAL ACTIVITY; ARTICLE; BACTERIUM ISOLATION; BLOOD CLOTTING; BLOOD COMPONENT; ENTEROCOCCUS FAECALIS; GRAM POSITIVE BACTERIUM; HUMAN; HUMAN CELL; IN VITRO STUDY; MINIMUM INHIBITORY CONCENTRATION; MOUTH CAVITY; MOUTH FLORA; NONHUMAN; NORMAL HUMAN; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS ORALIS; THROMBOCYTE ACTIVATION; CYTOLOGY; DRUG EFFECTS; MICROBIAL SENSITIVITY TEST; MICROBIAL VIABILITY; MICROBIOLOGY; MOUTH; PHYSIOLOGY; THROMBOCYTE RICH PLASMA;

ANTIINFECTIVE AGENT;

ANTI-BACTERIAL AGENTS; ENTEROCOCCUS FAECALIS; HUMANS; MICROBIAL SENSITIVITY TESTS; MICROBIAL VIABILITY; MOUTH; PLATELET ACTIVATION; PLATELET-RICH PLASMA; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS ORALIS;

EID: 84907195334     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0107813     Document Type: Article

References (38)

1. Anitua E, Andia I, Ardanza B, Nurden P, Nurden AT (2004) Autologous platelet as a source of proteins for healing and tissue regeneration. Thromb Haemost 91: 4-15.
2. Intini G (2009) The use of platelet-rich plasma in bone reconstruction therapy. Biomaterials 30: 4956-4966.
3. Del Fabbro M, Ceresoli V, Lolato A, Taschieri S (2012) Effect of platelet concentrate on quality of life after periradicular surgery: a randomized clinical study. J Endod 38: 733-739.
4. Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, et al. (2006) Plateletrich fibrin (PRF): a second-generation platelet concentrate. Part III: leukocyte activation: new feature for platelet concentrates? Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101: 51-55.
5. El-Sharkawy H, Kantarci A, Deady J, Hasturk H, Liu H, et al. (2007) Plateletrich plasma: growth factors and pro- and anti-inflammatory properties. J Periodontol 78: 661-669.
6. Aktan I, Dunkel B, Cunningham FM (2013) Equine platelets inhibit Escherichia coli growth and can be activated by bacterial lipopolysaccharide and lipoteichoic acid although superoxide anion production does not occur and platelet activation is not associated with enhanced production by neutrophils. Vet Immunol Immunopathol 152: 209-217.
7. Álvarez ME, López C, Giraldo CE, Samudio I, Carmona JU (2011) In vitro bactericidal activity of equine platelet concentrates, platelet poor plasma, and plasma against methicillin-resistant Staphylococcus aureus. Arch Med Vet 43: 155-161.
8. Anitua E, Alonso R, Girbau C, Aguirre JJ, Murozabal F, et al. (2012) Antibacterial effect of plasma rich in growth factors (PRGF-Endoret) against Staphylococcus aureus and Staphylococcus epidermidis strains. Clin Exp Dermatol 37: 652-657.
9. Bielecki TM, Gazdzik TS, Arendt J, Szczepanski T, Krol W, et al. (2007) Antibacterial effect of autologous platelet gel enriched with growth factors and other active substances: an in vitro study. J Bone Joint Surg Br 89: 417-420.
10. Burnouf T, Chou ML, Wu YW, Su CY, Lee LW (2013) Antimicrobial activity of platelet (PLT)-poor plasma, PLT-rich plasma, PLT gel, and solvent/detergent-treated PLT lysate biomaterials against wound bacteria. Transfusion 53: 138-146.
11. Chen L, Wang C, Liu H, Liu G, Ran X (2013) Antibacterial effect of autologous platelet-rich gel derived from subjects with diabetic dermal ulcers in vitro. J Diabetes Res 2013: 269527. doi: 10.1155/2013/269527.
12. Cieslik-Bielecka A, Bielecki T, Gazdzik TS, Arendt J, Krol W, et al. (2009). Autologous platelets and leukocytes can improve healing of infected high-energy soft tissue injury. Transfus Apher Sci 41: 9-12.
13. Drago L, Bortolin M, Vassena C, Taschieri S, Del Fabbro M (2013) Antimicrobial activity of pure platelet-rich plasma against microorganisms isolated from oral cavity. BMC Microbiol 13: 47. doi: 10.1186/1471-2180-13-47.
14. Everts PA, Devilee RJ, Brown Mahoney C, Eeftinck-Schattenkerk M, Box HA, et al. (2006) Platelet gel and fibrin sealant reduce allogeneic blood transfusions in total knee arthroplasty. Acta Anaesthesiol Scand 50: 593-599.
15. Moojen DJ, Everts PA, Schure RM, Overdevest EP, van Zundert A, et al. (2008) Antimicrobial activity of platelet-leukocyte gel against Staphylococcus aureus. J Orthop Res 26: 404-410.
16. Rozalski MI, Micota B, Paszkiewicz M, Wieckowoska-Szakiel M, Rozalska B (2013) Antimicrobial/anti-biofilm activity of expired blood platelets and their released products. Postepy Hig Med Dosw 67: 321-325.
17. Tohidnezhad M, Varoga D, Podschun R, Wruck CJ, Seekamp A, et al. (2011) Thrombocytes are effectors of the innate immune system releasing human beta defensin-3. Injury 42: 682-686.
18. Tohidnezhad M, Varoga D, Wruck CJ, Podschun R, Sachweh BH, et al. (2012) Platelets display potent antimicrobial activity and release human beta-defensin 2. Platelets 23: 217-223.
19. Trowbridge CC, Stammers AH, Woods E, Yen BR, Klayman M, et al. (2005). Use of platelet gel and its effects on infection in cardiac surgery. J Extra Corpor Technol 37: 381-386.
20. Yuan T, Zhang CQ, Zeng BF (2008) Treatment of chronic femoral osteomyelitis with platelet-rich plasma (PRP): A case report. Transfus Apher Sci 38: 167-70.
21. Stuart CH, Schwartz SA, Beeson TJ, Owatz CB (2006) Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 32: 93-98.
22. Bornstein MM, Hakimi B, Persson GR (2008) Microbiological findings in subject with asymptomatic oral lichen planus: a cross-sectional comparative study. J Periodontol 79: 2347-2355.
23. Burnie JP, Brooks W, Donohoe M, Hodgetts S, al-Ghamdi A, et al. (1996) Defining antibody targets in Streptococcus oralis infection. Infect Immunol 64: 1600-1608.
24. Terpenning MS, Taylor GW, Lopatin DE, Kerr CK, Dominguez BL, et al. (2001) Aspiration pneumonia: dental and oral risk factors in an older veteran population. J Am Geriatr Soc 49: 557-563.
25. El-Solh AA, Pietrantoni C, Bhat A, Okada M, Zambon J, et al. (2004) Colonization of dental plaques: a reservoir of respiratory pathogens for hospital-acquired pneumonia in institutionalized elders. Chest 126: 1575-1582.
26. Smith AJ, Robertson D, Tang MK, Jackson MS, MacKenzie D, et al. (2003) Staphylococcus aureus in the oral cavity: a three-year retrospective analysis of clinical laboratory data. Br Dent J 195: 701-703.
27. Blair P, Flaumenhaft R (2009) Platelet alpha-granules: basic biology and clinical correlates. Blood Rev 23: 177-189.
28. Kraemer BF, Campbell RA, Schwertz H, Cody MJ, Franks Z, et al. (2011) Novel antibacterial activities of b-defensin 1 in human platelets: suppression of pathogen growth and signaling of neutrophil extracellular trap formation. Plos pathog 7: e1002355. doi: 10.1371/journal.ppat.1002355.
29. Levy O (2000) Antimicrobial proteins and peptides of blood: templates for novel antimicrobial agents. Blood 96: 2664-2672.
30. Radek K, Gallo R (2007) Antimicrobial peptides: natural effectors of the innate immune system. Semin Immunopathol 29: 27-43.
31. Tang YQ, Yeaman MR, Selsted ME (2002) Antimicrobial peptides from human platelets. Infect Immun 70: 6524-6533.
32. Tohidnezhad M, Varoga D, Wruck CJ, Brandenburg LO, Seekamp A, et al. (2011) Platelet-released growth factors can accelerate tenocyte proliferation and activate the antioxidant response element. Histochem Cell Biol 135: 453-460.
33. Yeaman MR (1997) The role of platelets in antimicrobial host defense. Clin Infect Dis 25: 951-968.
34. Dohan Ehrenfest DM, Rasmusson L, Albrektsson T (2009) Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leukocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol 27: 158-167.
35. Schnabel LV, Mohammed HO, Miller BJ, McDermott WG, Jacobson MS, et al. (2007) Platelet rich plasma (PRP) enhances anabolic gene expression patterns in flexor digitorum superficialis tendons. J Orthop Res 25: 230-240.
36. Daha MR (2010) Role of complement in innate immunity and infections. Crit Rev Immunol 30: 47-52.
37. Joiner KA, Brown EJ, Frank MM (1984) Complement and bacteria: chemistry and biology in host defense. Annu Rev Immunol 2: 461-491.
38. Wu T, Yeaman MR, Bayer AS (1994) In vitro resistance to platelet microbicidal protein correlates with endocarditis source among bacteremic staphylococcal and streptococcal isolates. Antimicrob Agents Chemother. 38(4): 729-32.