Detection of sulfonamide resistance genes via in situ PCR-FISH

Polish Journal of Microbiology

Volumn 63, Issue 2, 2014, Pages 167-173

  Gnida, Anna ^a   Kunda, Katarzyna ^a   Ziembińska, Aleksandra ^a   Łuczkiewicz, Aneta ^b   Felis, Ewa ^a   Surmacz Górska, Joanna ^a  

^a SILESIAN UNIVERSITY OF TECHNOLOGY   (Poland)

^b GDANSK UNIVERSITY OF TECHNOLOGY   (Poland)

Author keywords

Activated sludge; FISH; In situ PCR; Sulfamethoxazole resistance gene

Indexed keywords

SULFAMETHOXAZOLE; ANTIINFECTIVE AGENT; BACTERIAL PROTEIN; PRIMER DNA; SULFANILAMIDE; SULFANILAMIDE DERIVATIVE;

ACTIVATED SLUDGE; ANTIBIOTIC RESISTANCE; ARTICLE; CONTROLLED STUDY; FLUORESCENCE IN SITU HYBRIDIZATION; GENE; GENETIC ANALYSIS; ORGANISM COMMUNITY; POLYMERASE CHAIN REACTION; BACTERIUM; DRUG EFFECT; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; SEWAGE; WASTE WATER;

ANIMALIA;

ANTI-BACTERIAL AGENTS; BACTERIA; BACTERIAL PROTEINS; DNA PRIMERS; DRUG RESISTANCE, BACTERIAL; IN SITU HYBRIDIZATION, FLUORESCENCE; POLYMERASE CHAIN REACTION; SEWAGE; SULFANILAMIDES; WASTE WATER;

EID: 84906980977     PISSN: 17331331     EISSN: 25444646     Source Type: Journal    
DOI: 10.33073/pjm-2014-022     Document Type: Article

References (29)

1. Amann R.I., B.J. Binder, R.J. Olson, S.W. Chisholm, R. Devereux and D.A. Stahl. 1990. Combination of 16S rRNA-targeted oligonu-cleotide probes with fow cytometry for analyzing mixed microbial populations. Appl. Envir. Microbiol. 56: 1919-1925.
2. Antunes P., J. Machado, J.C. Sousa and L. Peixe. 2005. Dissemination of sulfonamide resistance genes (sul1, sul2, and sul3) in portuguese Salmonella enterica strains and relation with integrons. Antimicrob. Agents and Chemother. 49(2): 836-839.
3. Bhandari A., L. Close, W. Kim, R.P. Hunter, D.E. Koch and R. Surampalli. 2008. Occurrence of ciprofoxacin, sulfamethoxa-zole, and azithromycin in municipal wastewater treatment plants. Practice periodical of hazardous, toxic, and radioactive waste management 275
4. Bobrow M.N., T.D. Harris, K.J. Shaughnessy and G.J. Litt. 1989. Catalyzed reporter deposition, a novel method of signal amplification: application to immunoassays. J. Immunol. Methods 125: 279-285.
5. Brunton L., B. Chabner and B. Knollman. 2006. Goodman and Gilman's The Pharmacological Basis of Terapeutics. McGraw-Hill Professional, New York
6. Cheng F. And R.E. Hodson. 2001. In situ PCR/RT-PCR coupled with in situ hybridization for detection of functional gene and gene expression in prokaryotic cells, pp. 409-424. In: Paul H.J. (ed): Methods in Microbiology 30. Academic Press, San Diego, USA.
7. Czekalski N., T. Berthold, S. Caucci, A. Egli and H. Bürgmann. 2012. Increased levels of multiresistant bacteria and resistance genes afer wastewater treatment and their dissemination into Lake Geneva, Switzerland. Frontiers in Microbiology 3(106): 2-18.
8. Daims H., A. Brühl A., Amann R., Schleifer K.-H. And M. Wagner. 1999. The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: Development and evaluation of a more comprehensive probe set. Syst. Appl. Microbiol. 22: 434-444.
9. Daims H., K. Stoecker and M. Wagner. 2005. Fluorescence in situ hybridization for the detection of prokaryotes, pp. 213-239. In: Molecular Microbial Ecology, ed. A.M. Osborn and J. Smith, Advanced methods in molecular microbial ecology. Bios-Garland, Abingdon, United Kingdom.
10. da Silva M.F., I. Vaz-Moreira, M. Gonzalez-Pajuelo, O.C. Nunes and C.M. Manaia. 2007. Antimicrobial resistance patterns in Enterobacteriaceae isolated from an urban wastewater treatment plant. Microbiol. Ecol. 60: 166-176.
11. Grape M., L. Sundstrom and G. Kronvall. 2003. Sulphonamide resistance gene sul3 found in Escherichia coli isolates from human sources. J. Antimicrob. Chemother. 52: 1022-1024.
12. Hoa P.T.P., L. Nonaka, P.H. Viet and S. Suzuki. 2008. Detection of the sul1, sul2, and sul3 genes in sulfonamide-resistant bacteria from wastewater and shrimp ponds of north Vietnam. Sci. Total Environ. 405: 377-384.
13. Hodson R., W. Dustman, R.P. Garg and M.A. Moran. 1995. In situ PCR for visualization of microscale distribution of specific genes and gene products in prokaryotic communities. Appl. Environ. Microbiol. 61(11): 4074-4082.
14. Hoshino T., S. Tsuneda, A. Hirata and Y. Inamori. 2003. In situ PCR for visualizing distribution of a functional gene "amoA" in a biofilm regardless of activity. J. Biotechnol. 105(1-2): 33-40.
15. van Hoek A. And H. Aarts. 2008. Microarray-based detection of antibiotic resistance genes in Salmonella. Food Anal. Methods. 1(2): 95-108.
16. Kadlec K., C. Kehrenberg and S. Schwarz. 2005. Molecular basis of resistance to trimethoprim, chloramphenicol and sulphonamides in Bordetella bronchiseptica. J. Antimicrob. Chemother. 56: 485-490.
17. Łuczkiewicz A., E. Felis, Z. Ziembińska, A. Gnida, E. Kotlarska, K. Olańczuk-Neyman and J. Surmacz-Górska. 2013. Resistance of Escherichia coli and Enterococcus spp. To selected antimicrobial agents present in municipal wastewater. J. Wat. Health, doi:10.2166/wh.2013.130
18. Majtan T., L. Majtanova, J. Timko and V. Majtan. 2007. Oligonu-cleotide microarray for molecular characterization and genotyping of Salmonella spp. Strains. J. Antimicrob. Chemother. 60: 937-946.
19. Mohapatra H., S.S. Mohapatra, C.K. Mantri, R.R. Colwell and D.V. Singh. 2008. Vibrio cholerae non-O1, non-O139 strains isolated before 1992 from Varanasi, India are multiple drug resistant, contain intSXT, dfr18 and aadA5 genes. Environ. Microbiol. 10: 866-873.
20. Pei R., S.C. Kim, K.H. Carlson and A. Pruden. 2006. Efect of river landscape on the sediment concentrations of antibiotics and corresponding antibiotic resistance genes (ARG). Wat. Res. 40(12): 2427-2435.
21. Perreten V. And P. Boerlin. 2003. A new sulfonamide resistance gene (sul3) in Escherichia coli is widespread in the pig population of Switzerland. Antimicrob. Agents Chemother. 47(3): 1169-1172.
22. Skold O. 2000. Sulfonamide resistance Sulfonamide resistance: mechanisms and trends. Drug Resist. Updat. 3: 155-160.
23. Srinivasan V., H.M. Nam, L.T. Nguyen, B. Tamilselvam, S.E. Murinda and S.P. Oliver. 2005. Prevalence of antimicrobial resistance genes in Listeria monocytogenes isolated from dairy farms. Foodborne Pathog. Dis. 2: 201-221.
24. Stoecker K., C. Dorninger, H. Daims and M. Wagner. 2010. Double labeling of oligonucleotide probes for fuorescence in situ hybridization (DOPE-FISH) improves signal intensity and increases rRNA accessibility. Appl. Environ. Microbiol. 76(3), 922-926.
25. Szczepanowski R., B. Linke, I. Krahn, K.-H. Gartemann, T. Gütz-kow, W. Eichler, A. Pühler and A. Schlüter. 2009. Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater plant bacteria showing reduced susceptibility to selected antibiotics. Microbiol. 155: 2306-2319.
26. Suzuki S. And P.T.P. Hoa. 2012. Distribution of quinolones, sulfon-amides, tetracyclines in aquatic environment and antibiotic resistance in Indochina. Front Microbiol. 3: 67.
27. WHO. 2013. Antimicrobial resistance. Fact sheet N°194.
28. Zhang X-X., T. Zhang and H. Fang. 2009. Antibiotic resistance genes in water environment. Appl. Microbiol. Biotechnol. 82: 397-414.
29. Zwirglmaier K., W. Ludwig and K.H. Schleifer. 2004. Recognition of individual genes in a single bacterial cell by fuorescence in situ hybridization-RING-FISH. Mol. Microbiol. 51(1): 89-96.