Phenotypic and genotypic characterization of nosocomial isolates of Staphylococcus aureus with reference to methicillin resistance

Tropical Journal of Pharmaceutical Research

Volumn 13, Issue 8, 2014, Pages 1239-1246

  Salem Bekhit, Mounir M ^a,b  

^a KING SAUD UNIVERSITY   (Saudi Arabia)

^b AL AZHAR UNIVERSITY   (Egypt)

Author keywords

Antibiotics; femB; mecA; Methicillin resistance; Staphylococcal infections; Staphylococcus aureus

Indexed keywords

EID: 84906852802     PISSN: 15965996     EISSN: 15969827     Source Type: Journal    
DOI: 10.4314/tjpr.v13i8.7     Document Type: Article

References (29)

1. Al-Jumaily E, Mohamed D, Khanaka H. Molecular epidemiology and Antibiotic susceptibility patterns of c1inical strains of methicillin resistant Staphylococcus aureus (MRSA) in Sulaimani city-Iraq. Glob Adv Res J Microbiol 2012; 6: 81-89.
2. World Health Organization (WHO). 2011 Public Health Importance of Antimicrobial Resistance. See: http://www.who.int/drugresistance/AMR_Importance/ en/index.html
3. Madani T, Al-Abdullah N, Al-Sanousi A, Ghabrah T, Bajunid H. Methicillin-resistant Staphylococcus aureus in two tertiary-care centers in Jeddah, Saudi Arabia. Infect Control Hosp Epidemiol 2002001; 14: 211-216.
4. Liu C, Bayer A, Cosgrove S, Chambers H. Clinical Practice Guidelines by the Infectious Diseases Society of America for the Treatment of Methicillin-Resistant Staphylococcus aureus Infections in Adults and Children. Clin Infect Dis 2011; 1-38.
5. Rehm S. Staphylococcus aureus: The new adventures of a legendary pathogen. Cleveland Clinic Journal of Medicine, 2008; 75: 177-192.
6. Johnson A. Methicillin-resistant Staphylococcus aureus: the European landscape J Antimicrob Chemother 2011; 66(Suppl 4): iv43-iv48.
7. Verhoef J, Beaujean D, Block H, Baars A, Meyler A, Van der Werken C. Dutch approach to methicillin-resistant Staphylococcus aureus. Eur J Clin Infect Dis 1999; 18: 461-466.
8. Hartman B, Tomasz A. Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus. J Bacteriol 1984; 158: 513-516.
9. Maes N, Magdalena J, Rottiers S, De Gheldre Y, Struelens MJ. Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulasenegative staphylococci and to determine methicillin resistance from blood cultures. J Clin Microbiol 2002; 40: 1514-1517.
10. Gradelski E, Valera L, Aleksunes L, Bonner D, Fung-Tomc J. Correlation between the genotype and phenotypic categorization of staphylococci based on the methicillin susceptibility and resistance. J Clin Microbiol 2001; 39: 2961-2963.
11. Hegde S, Shrader T. FemABX Family Members Are Novel Nonribosomal Peptidyltransferases and Important Pathogen-Specific Drug Targets. J Biol Chem, 2001; 10: 6998-7003.
12. El-Sharif A, Ashour H. Colonization and Infection in Intravenous and Inhalational Opiate Drug Abusers Community-Acquired Methicillin-Resistant Staphylococcus aureus (CA-MRSA). Exp Biol Med 2008; 233: 874-880.
13. Awadalla H, Khalil I, Bassim H, Ahmed M, Wahba L. Molecular typing of methicilin-resistant Staphylococcus aureus isolates at Ain Shams University Hospital, Egypt. Afr J Microbiol Res 2010; 4: 1639-1646.
14. Helal ZH, Gomaa FA, Radwan S. New Rapid Method for Differentiation of MRSA and SSA by PCR Restriction Analysis of 920 bp of Dnaj genePrime J Microbiol Res 2012; 2(5): 141-146.
15. Shehab El-Din S, El-Shafey E, El-Hadidy M, Zaghloul H. Methicillin Resistant Staphylococcus aureus: A problem in the burns unit. Egypt J Plast Reconstr Surg 2003; 27: 1-10.
16. Rushdy A, Salama M, Othman A. Detection of Methicillin/Oxacillin Resistant Staphylococcus aureus Isolated from Some Clinical Hospitals in Cairo Using Meca/Nuc Genes and Antibiotic Susceptibility Profile Int J Agri Biol 2007; 9: 800-806.
17. Mohanasoundaram K, Lalitha M. Comparisonof phenotypic versus genotypic methods in the detection of methicillin resistance in Staphylococcus aureus. Indian J Med Res 2008; 127: 78-84.
18. Kloos W, Lambe D. Manual of Clinical Microbiology, 5th ed. Washington, D.C.: American Society for Microbiology, 1991 pp 222-237.
19. Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimi-crobial susceptibility testing: seventeenth informational supplement [document M100-S17]. Wayne, PA: CLSI, 2007.
20. Ausubel FM, Brent R, Kingston R, Moore DE, Seidman, Smith JA., Strunt K. Short Protocols in Molecular Biology, 4th edn. John Wiley and Sons Inc. 1999, New York.
21. Jonas D, Speck M, Daschner F, Grundmann H. Rapid PCR-Based Identification of Methicillin-Resistant Staphylococcus aureus from screening swabs. J Clin Microbiol 2002; 40: 1821-1823.
22. Gad Y, Talaat N. Methecillin Resistant Staphylococcal aureus (MRSA) isolates from a hepatology intensive care unit in Egypt: A silent killer. E&C Hepatol 2012; 8: 35-44
23. Voss A, Doebbeling B. The worldwide prevalence of methicillin-resistant Staphylococcus aureus. Intern J Antimicrob Agents 1995; 5: 101-106.
24. Low D, Nadler H. A review of in vitro antibacterial activity of quinupristin/dalfopristin against methicillinsusceptible and resistant Staphylococcus aureus. J Antimicrob Chemother 1997; 39 (Suppl. A), 53-58.
25. Fraise A. Guidelines for the control of methicillin-resistant Staphylococcus aureus. J Antimicrob Chemother 1998; 42: 287-289.
26. Marple R, Speller D, Cookson B. Prevalence of mupirocin resistance in Staphylococcus aureus. J Hosp Infect 1995; 29: 153-154.
27. Al-Talib H, Chan Y, Alyaa A, Al-Jashamy K, Ravichandran M. A Pentaplex PCR Assay for the Detection of Methicillin-Resistant Staphylococcus aureus and Panton-Valentine Leucocidin BMC Microbiol 2009; 9: 113.
28. Kobayashi N, Wu H, Kojima K, Yagihashi A, Kurokawa I. Detection of mecA, femA, and femB Genes in Clinical Strains of Staphylococci Using Polymerase Chain Reaction Epidem Infect 1994; 113: 259-266.
29. Gutierrez K, Halpern MS, Sarnquist C, Soni S, Arroyo AC, Maldonado Y. Staphylococcal infections in children, california, USA, 1985-2009. Emerg Infect Dis. 2013; 19: 10-20.