Disrupted intestinal microbiota and intestinal inflammation in children with cystic fibrosis and its restoration with lactobacillus gg: A randomised clinical trial

PLoS ONE

Volumn 9, Issue 2, 2014, Pages

  Bruzzese, Eugenia ^a   Callegari, Maria Luisa ^b   Raia, Valeria ^a   Viscovo, Sara ^a   Scotto, Riccardo ^a   Ferrari, Susanna ^b   Morelli, Lorenzo ^b   Buccigrossi, Vittoria ^a   Lo Vecchio, Andrea ^a   Ruberto, Eliana ^a   Guarino, Alfredo ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^b UNIVERSITÀ CATTOLICA DEL SACRO CUORE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; CALGRANULIN; DNA; NITRIC OXIDE; PROBIOTIC AGENT; STEROID;

ARTICLE; BACTEROIDES UNIFORMIS; BACTEROIDES VULGATUS; BIFIDOBACTERIUM; BIFIDOBACTERIUM ADOLESCENTIS; BIFIDOBACTERIUM CATENULATUM; BODY WEIGHT; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; CYSTIC FIBROSIS; DENATURING GRADIENT GEL ELECTROPHORESIS; DISEASE EXACERBATION; DRUG EFFICACY; ENTERITIS; EUBACTERIUM; EUBACTERIUM RECTALE; FAECALIBACTERIUM PRAUSNITZII; FLUORESCENCE IN SITU HYBRIDIZATION; GENE AMPLIFICATION; GENOTYPE; HUMAN; INCIDENCE; INTESTINE FLORA; LACTOBACILLUS; MALE; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; RANDOMIZED CONTROLLED TRIAL; REAL TIME POLYMERASE CHAIN REACTION; SPECIES RICHNESS; CASE CONTROL STUDY; DOUBLE BLIND PROCEDURE; DRUG EFFECTS; FECES; FEMALE; GENETICS; INFLAMMATION; INTESTINE; MICROBIOLOGY; MICROFLORA; PHYSIOLOGY; PRESCHOOL CHILD;

CASE-CONTROL STUDIES; CHILD; CHILD, PRESCHOOL; CYSTIC FIBROSIS; DOUBLE-BLIND METHOD; FECES; FEMALE; HUMANS; INFLAMMATION; INTESTINES; LACTOBACILLUS; MALE; MICROBIOTA; PROBIOTICS;

EID: 84906833163     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0087796     Document Type: Article

References (34)

1. Guarino A, Giannattasio A (2011) New molecular approaches in the diagnosis of acute diarrhea: advantages for clinicians and researches.Curr Opin Gastroenterol. 27: 24-9.
2. Musso G, Gambino R, Cassader M (2011) Interactions between gut microbiota and host metabolism predisposing to obesity and diabetes. Ann Rev Med.62: 361-80.
3. Bäckhed F, Manchester JK, Semenkovich CF, Gordon JI (2007) Mechanisms underlying the resistance to diet-induced obesity in germ-free mice. Proc Natl Acad Sci U S A 104: 979-84.
4. Turnbaugh PJ, Bäckhed F, Fulton L, Gordon JI (2008) Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome. Cell Host Microbe 3: 213-223.
5. Turnbaugh PJ, Hamady M, Yatsunenko T, Cantarel BL, Duncan A, et al (2009) A core gut microbiome in obese and lean twins. Nature 457: 480-484.
6. Lee JM, Leach ST, Katz T, Day AS, Jaffe A, et al (2012) Update of faecal markers of inflammation in children with cystic fibrosis. Mediators Inflamm 948367.
7. Bruzzese E, Raia V, Gaudiello G, Polito G, Buccigrossi V, et al. (2004) Intestinal inflammation is a frequent feature of cystic fibrosis and is reduced by probiotic administration. Aliment Pharmacol Ther 20: 813-819.
8. Werlin SL, Benuri-Silbiger I, Kerem E, Adler SN, Goldin E, et al. (2010) Evidence of Intestinal Inflammation in Patients With Cystic Fibrosis. J Pediatr Gastroenterol Nutr 51: 304-308.
9. Scanlan PD, Buckling A, Kong W, Wild Y, Lynch SV, et al (2012) Gut dysbiosis in cystic fibrosis. J Cyst Fibros. 11: 454-5.
10. Duytschaever G, Huys G, Bekaert M, Boulanger L, De Boeck K, et al (2011) Cross-sectional and longitudinal comparisons of the predominant fecal microbiota compositions of a group of pediatric patients with cystic fibrosis and their healthy siblings. Appl Environ Microbiol. 77: 8015-24.
11. Madan JC, Koestler DC, Stanton BA, Davidson L, Moulton LA, et al. (2012) Serial analysis of the gut and respiratory microbiome in Cystic Fibrosis in infancy: interaction between intestinal and respiratory tracts and impact of nutritional exposures. MBio.3 pii: e00251-12.
12. Duytschaever G, Huys G, Bekaert M, Boulanger L, De Boeck K, et al (2012) Dysbiosis of bifidobacteria and Clostridium cluster XIVa in the cystic fibrosis fecal microbiota. J Cyst Fibros. pii: S1569-1993(12)00183-X.
13. Bruzzese E, Raia V, Spagnuolo MI,Volpicelli M, De Marco G, et al. (2007) Effect of Lactobacillus GG supplementation on pulmonary exacerbations in patients with cystic fibrosis: a pilot study. Clin Nutr 26: 322-328.
14. Weiss B, Bujanover Y, Yahav Y, Vilozni D, Fireman E, et al. (2010) Probiotic supplementation affects pulmonary exacerbations in patients with cystic fibrosis: a pilot study. Pediatr Pulmonol. 45: 536-540.
15. Belenguer A, Duncan SH, Holtrop G, Anderson SE, Lobley GE, et al. (2007) Impact of pH on lactate formation and utilization by human fecal microbial communities. Appl Environ Microbiol. 73: 6526-6533.
16. Cox MJ, Huang YJ, Fujimura KE, Liu JT, McKean M, et al (2010) Lactobacillus casei abundance is associated with profound shifts in the infant gut microbiome. PLoS One. 5: e8745.
17. Kerem E. (2005) Pharmacological induction of CFTR function in patients with cystic fibrosis: mutation-specific therapy. Pediatr Pulmonol 40: 183-196.
18. Fagerberg UL, Loof L, Merzoug RD, Hansson LO, Finkel Y (2003) Fecal calprotectin levels in healthy children studied with an improved assay. J Pediatr Gastroenterol Nutr 37: 468-472.
19. Canani RB, Cirillo P, Bruzzese E, Graf M, Terrin G, et al. (2002) Nitric oxide production in rectal dialysate is a marker of disease activity and location in children with inflammatory bowel disease. Am J Gastroenterol 97: 1574-1576.
20. Walter J, Tannock GW, Tilsala-Timisjarvi A, Rodtong S, Loach DM, et al. (2000) Detection and identification of gastrointestinal Lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Appl Environ Microbiol. 66: 297-303.
21. Bartosch S, Fite A, Macfarlane GT, McMurdo ME (2004) Characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using real-time PCR and effects of antibiotic treatment on the fecal microbiota. Appl Environ Microbiol. 70: 3575-3581.
22. Ahmed S, Macfarlane GT, Fite A, McBain AJ, Gilbert P, et al. (2007) Mucosaassociated bacterial diversity in relation to human terminal ileum and colonic biopsy samples. Appl Environ Microbiol. 73: 7435-7442.
23. Wu X, Ma C, Han L, Nawaz M, Gao F, et al. (2010) Molecular characterisation of the faecal microbiota in patients with type II diabetes. Curr Microbiol. 61: 69-78.
24. Penders J, Vink C, Driessen C, London N, Thijs C, et al. (2005) Quantification of Bifidobacterium spp., Escherichia coli and Clostridium difficile in faecal samples of breast-fed and formula-fed infants by real-time PCR. FEMS Microbiol Lett. 243: 141-147.
25. Sokol H, Pigneur B, Watterlot L, Lakhdari O, Bermúdez-Humarán LG, et al. (2008) Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients. Proc Natl Acad Sci U S A. 105: 16731-6.
26. Swidsinski A, Loening-Baucke V, Verstraelen H, Osowska S, Doerffel Y (2008) Biostructure of fecal microbiota in healthy subjects and patients with chronic idiopathic diarrhea. Gastroenterology. 135: 568-79.
27. Daims H, Brühl A, Amann R, Schleifer KH, Wagner M (1999) The domainspecific probe EUB338 is insufficient for the detection of all Bacteria: development and evaluation of a more comprehensive probe set. Syst Appl Microbiol. 22: 434-44.
28. Scanlan PD, Shanahan F, O'Mahony C, Marchesi JR (2006) Cultureindependent analyses of temporal variation of the dominant fecal microbiota and targeted bacterial subgroups in Crohn's disease. J Clin Microbiol. 44: 3980-3988.
29. Buccigrossi V, Nicastro E, Guarino A (2013) Functions of intestinal microflora in children. Curr Opin Gastroenterol. 29(1): 31-8.
30. Marteau P (2009) Bacterial flora in inflammatory bowel disease. Dig Dis.Suppl 1: 27 99-103.
31. Takaishi H, Matsuki T, Nakazawa A, Takada T, Kado S, et al. (2008) Imbalance in intestinal microflora constitution could be involved in the pathogenesis of inflammatory bowel disease. Int J Med Microbiol 298(5-6): 463-72.
32. Dicksved J, Halfvarson J, Rosenquist M, Järnerot G, Tysk C, et al. (2008) Molecular analysis of the gut microbiota of identical twins with Crohn's disease. ISME J 2: 716-727.
33. Conte MP, Schippa S, Zamboni I, Penta M, Chiarini F, et al. (2006) Gutassociated bacterial microbiota in paediatric patients with inflammatory bowel disease. Gut 55: 1760-1767.
34. Noor SO, Ridgway K, Scovell L, Kemsley EK, Lund EK, et al. (2010) Ulcerative colitis and irritable bowel patients exhibit distinct abnormalities of the gut microbiota. BMC Gastroenterol 10: 134.