Design and validation of a real-time RT-PCR for the simultaneous detection of enteroviruses and parechoviruses in clinical samples

Journal of Virological Methods

Volumn 208, Issue , 2014, Pages 125-128

  Cabrerizo, María ^a   Calvo, Cristina ^b   Rabella, Nuria ^c   Muñoz Almagro, Carmen ^d   del Amo, Eva ^d   Pérez Ruiz, Mercedes ^e   Sanbonmatsu Gámez, Sara ^e   Moreno Docón, Antonio ^f   Otero, Almudena ^a   Trallero, Gloria ^a   Rabella, N ^g   Marti N ^g   Munoz Almagro C ^g   del Amo, E ^g   Roda, D ^g   Perez E ^g   Fons, C ^g   Martinez Rienda I ^h   Soria, L M ^h   Ojeda, E ^g   Megias, G ^g   Cilla, A ^g   Gomez Saez F ^g   Perez Ruiz M ^e   Sanbonmatsu, S ^e   Perez C ⁱ   Pena, M J ⁱ   Colino, E ⁱ   Romero, M P ^j   Garcia M L ^b   Calvo, C ^b   Aguado, I ^b   Menasalvas A I , ^f   Moreno Docon A ^f   Garcia Costa J ^g   Reis Iglesias, L ^g   Rey Cao, S ^g   Portugues M M ^g   Gonzalez Duran M L ^g   Omenaca M ^k   Cabrerizo, M ^l   Trallero, G ^l   Otero, A ^l   Fedele, G ^l   Varela, C ^l   Saiz M ^m   more..

^a INSTITUTO DE SALUD CARLOS III   (Spain)

^b HOSPITAL SEVERO OCHOA   (Spain)

^c HOSPITAL DE LA SANTA CREU I SANT PAU   (Spain)

^d Sant Joan de Déu Nursing s School University   (Spain)

^e HOSPITAL UNIVERSITARIO VIRGEN DE LAS NIEVES   (Spain)

^f HOSPITAL UNIVERSITARIO VIRGEN DE LA ARRIXACA   (Spain)

^g HU de Cruces ^*

^h HOSPITAL DE CRUCES   (Spain)

ⁱ Hospital de Gran Canaria   (Spain)

^j HOSPITAL UNIVERSITARIO LA PAZ   (Spain)

^k H Miguel Servet   (Spain)

^l INSTITUTO DE SALUD CARLOS III   (Spain)

^m CENTRO DE BIOLOGÍA MOLECULAR SEVERO OCHOA   (Spain)

more..

Author keywords

Clinical samples; Enterovirus; Parechovirus; Real time RT PCR; Young children

Indexed keywords

COMPLEMENTARY DNA; OLIGONUCLEOTIDE PROBE; PRIMER DNA; UNTRANSLATED RNA; VIRUS RNA;

ARTICLE; CONTROLLED STUDY; CROSS REACTION; DNA SYNTHESIS; ENTEROVIRUS; FEVER; HEPATITIS A VIRUS; HUMAN; LIMIT OF DETECTION; MENINGITIS; MOLECULAR TYPING; NONHUMAN; PARECHOVIRUS; POLYMERASE CHAIN REACTION SYSTEM; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; RHINOVIRUS; SENSITIVITY AND SPECIFICITY; SEPSIS; VALIDITY; VIRUS CULTURE; VIRUS DETECTION; VIRUS ISOLATION; VIRUS STRAIN; 5' UNTRANSLATED REGION; BLOOD SAMPLING; CEREBROSPINAL FLUID ANALYSIS; CHILD; ECHO VIRUS; RELIABILITY; THROAT CULTURE; VALIDATION STUDY; VIRUS GENE; CLASSIFICATION; GENETICS; INFANT; ISOLATION AND PURIFICATION; MOLECULAR DIAGNOSIS; OLIGONUCLEOTIDE PROBE; PICORNAVIRIDAE INFECTIONS; PRESCHOOL CHILD; PROCEDURES; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIROLOGY;

CHILD, PRESCHOOL; DNA PRIMERS; ENTEROVIRUS; HUMANS; INFANT; MOLECULAR DIAGNOSTIC TECHNIQUES; OLIGONUCLEOTIDE PROBES; PARECHOVIRUS; PICORNAVIRIDAE INFECTIONS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, UNTRANSLATED; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

EID: 84906816611     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2014.08.008     Document Type: Article

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