Purine salvage in the apicomplexan Sarcocystis neurona, and generation of hypoxanthine-xanthine-guanine phosphoribosyltransferase-deficient clones for positive-negative selection of transgenic parasites

Parasitology

Volumn 141, Issue 11, 2014, Pages 1399-1405

  Dangoudoubiyam, Sriveny ^a   Zhang, Zijing ^a   Howe, Daniel K ^a  

^a UNIVERSITY OF KENTUCKY   (United States)

Author keywords

Apicomplexa; HXGPRT; Purine salvage; Sarcocystis neurona; Transfection

Indexed keywords

6 THIOXANTHINE; ADENOSINE KINASE; ADENOSINE MONOPHOSPHATE DEAMINASE; ADENOSINE PHOSPHATE; ADENOSUCCINATE LYSASE; ADENYLOSUCCINATE SYNTHASE; GUANINE DERIVATIVE; GUANOSINE PHOSPHATE; HYPOXANTHINE DERIVATIVE; HYPOXANTHINE XANTHINE GUANINE PHOSPHORIBOSYLTRANSFERASE; INOSINATE DEHYDROGENASE; MYCOPHENOLIC ACID; PHOSPHORIBOSYLTRANSFERASE; PURINE; PURINE NUCLEOSIDE PHOSPHORYLASE; UNCLASSIFIED DRUG; XANTHINE DERIVATIVE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; GLYCOSYLTRANSFERASE; HYPOXANTHINE-GUANINE-XANTHINE PHOSPHORIBOSYLTRANSFERASE; PROTOZOAL PROTEIN; PURINE DERIVATIVE;

CELL CLONE; CLONING; CONFERENCE PAPER; CONTROLLED STUDY; EXON; GENE LOCUS; GENE SEQUENCE; GENE TARGETING; GENETIC TRANSFECTION; MEROZOITE; MICROBIAL GENETICS; MICROBIAL GROWTH; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PARASITE PHENOMENA AND FUNCTIONS; PRIORITY JOURNAL; PURINE SALVAGE; PURINE SYNTHESIS; SARCOCYSTIS NEURONA; SCHIZONT; SIGNAL TRANSDUCTION; TOXOPLASMA GONDII; TRANSGENIC ORGANISM; WESTERN BLOTTING; WILD TYPE; BIOINFORMATICS; GENE DISRUPTION; GENE INACTIVATION; MICROBIAL GENE; MICROBIAL GENOME; REVIEW; ANIMAL; ENZYMOLOGY; GENETIC COMPLEMENTATION; GENETICS; HORSE; METABOLISM; PARASITOLOGY; SARCOCYSTIS; SARCOCYSTOSIS; TOXOPLASMA; TRANSGENIC ANIMAL;

APICOMPLEXA; SARCOCYSTIS NEURONA;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; GENE KNOCKOUT TECHNIQUES; GENETIC COMPLEMENTATION TEST; HORSES; HYPOXANTHINES; PENTOSYLTRANSFERASES; PROTOZOAN PROTEINS; PURINES; SARCOCYSTIS; SARCOCYSTOSIS; TOXOPLASMA; TRANSFECTION;

EID: 84906789773     PISSN: 00311820     EISSN: 14698161     Source Type: Journal    
DOI: 10.1017/S0031182014000687     Document Type: Conference Paper

References (23)

1. Beech, J. (1974). Equine protozoan encephalomyelitis. Veterinary Medicine, Small Animal Clinical 69, 1562, 1564-1566.
2. Berens, R. L., Krug, E. C. and Marr, J. J. (1995). Purine and pyrimidine metabolism. In Biochemistry and Molecular Biology of Parasites (ed. Marr, J. J. and Müller, M.), pp. 323-336. Academic Press, London, UK.
3. Bhatti, M. M. and Sullivan, W. J., Jr. (2005). Histone acetylase GCN5 enters the nucleus via importin-alpha in protozoan parasite Toxoplasma gondii. Journal of Biological Chemistry 280, 5902-5908.
4. Chaudhary, K., Darling, J. A., Fohl, L. M., Sullivan, W. J., Jr., Donald, R. G., Pfefferkorn, E. R., Ullman, B. and Roos, D. S. (2004). Purine salvage pathways in the apicomplexan parasite Toxoplasma gondii. Journal of Biological Chemistry 279, 31221-31227.
5. Cheadle, M. A., Yowell, C. A., Sellon, D. C., Hines, M., Ginn, P. E., Marsh, A. E., Dame, J. B. and Greiner, E. C. (2001). The striped skunk (Mephitis mephitis) is an intermediate host for Sarcocystis neurona. International Journal for Parasitology 31, 843-849.
6. Cheadle, M. A., Ginn, P. E., Lindsay, D. S. and Greiner, E. C. (2002). Neurologic disease in gamma-interferon gene knockout mice caused by Sarcocystis neurona sporocysts collected from opossums fed armadillo muscle. Veterinary Parasitology 103, 65-69.
7. Donald, R. G. and Roos, D. S. (1998). Gene knock-outs and allelic replacements in Toxoplasma gondii: HXGPRT as a selectable marker for hit-and-run mutagenesis. Molecular and Biochemical Parasitology 91, 295-305.
8. Donald, R. G. K., Carter, D., Ullman, B. and Roos, D. S. (1996). Insertional tagging, cloning, and expression of the Toxoplasma gondii hypoxanthine-xanthine-guanine phosphoribosyltransferase gene. Journal of Biological Chemistry 271, 14010-14019.
9. Dubey, J. P., Saville, W. J., Lindsay, D. S., Stich, R.W., Stanek, J. F., Speer, C. A., Rosenthal, B. M., Njoku, C. J., Kwok, O. C., Shen, S. K. and Reed, S. M. (2000). Completion of the life cycle of Sarcocystis neurona. Journal of Parasitology 86, 1276-1280.
10. Dubey, J. P., Lindsay, D. S., Saville, W. J., Reed, S. M., Granstrom, D. E. and Speer, C. A. (2001a). A review of Sarcocystis neurona and equine protozoal myeloencephalitis (EPM). Veterinary Parasitology 95, 89-131.
11. Dubey, J. P., Saville, W. J., Stanek, J. F., Lindsay, D. S., Rosenthal, B. M., Oglesbee, M. J., Rosypal, A. C., Njoku, C. J., Stich, R.W., Kwok, O. C., Shen, S. K., Hamir, A. N. and Reed, S. M. (2001b). Sarcocystis neurona infections in raccoons (Procyon lotor): evidence for natural infection with sarcocysts, transmission of infection to opossums (Didelphis virginiana), and experimental induction of neurologic disease in raccoons. Veterinary Parasitology 100, 117-129.
12. Fenger, C. K. (1998). Treatment of equine protozoal myeloencephalitis. Compendium on Continuing Education for the Practising Veterinarian 20, 1154-1157.
13. Gaji, R. Y. and Howe, D. K. (2009). The heptanucleotide motif GAGACGC is a key component of a cis-acting promoter element that is critical for SnSAG1 expression in Sarcocystis neurona. Molecular and Biochemical Parasitology 166, 85-88.
14. Gaji, R. Y., Zhang, D., Breathnach, C. C., Vaishnava, S., Striepen, B. and Howe, D. K. (2006). Molecular genetic transfection of the coccidian parasite Sarcocystis neurona. Molecular and Biochemical Parasitology 150, 1-9.
15. Gherardi, A. and Sarciron, M. E. (2007). Molecules targeting the purine salvage pathway in Apicomplexan parasites. Trends in Parasitology 23, 384-389.
16. Hoane, J. S., Carruthers, V. B., Striepen, B., Morrison, D. P., Entzeroth, R. and Howe, D. K. (2003). Analysis of the Sarcocystis neurona microneme protein SnMIC10: protein characteristics and expression during intracellular development. International Journal for Parasitology 33, 671-679.
17. Howe, D. K. and Sibley, L. D. (1997). Development of molecular genetics for Neospora caninum: a complementary system to Toxoplasma gondii. Methods: A Companion to Methods in Enzymology 13, 123-133.
18. Krug, E. C., Marr, J. J. and Berens, R. L. (1989). Purine metabolism in Toxoplasma gondii. Journal of Biological Chemistry 264, 10601-10607.
19. MacKay, R. J. (2006). Equine protozoal myeloencephalitis: treatment, prognosis, and prevention. Clinical Techniques in Equine Practice 5, 9-16.
20. Pfefferkorn, E. R. and Borotz, S. E. (1994). Toxoplasma gondii: characterization of a mutant resistant to 6-thioxanthine. Experimental Parasitology 79, 374-382.
21. Pfefferkorn, E. R. and Pfefferkorn, L. C. (1978). The biochemical basis for resistance to adenine arabinoside in a mutant of Toxoplasma gondii. Journal of Parasitology 64, 486-492.
22. Pfefferkorn, E. R., Bzik, D. J. and Honsinger, C. P. (2001). Toxoplasma gondii: mechanism of the parasitostatic action of 6-thioxanthine. Experimental Parasitology 99, 235-243.
23. Vaishnava, S., Morrison, D. P., Gaji, R. Y., Murray, J. M., Entzeroth, R., Howe, D. K. and Striepen, B. (2005). Plastid segregation and cell division in the apicomplexan parasite Sarcocystis neurona. Journal of Cell Science 118, 3397-3407.