Enhancement of the activity of enzyme immobilized on polydopamine-coated iron oxide nanoparticles by rational orientation of formate dehydrogenase

Journal of Biotechnology

Volumn 188, Issue , 2014, Pages 36-41

  Gao, Xin ^a   Ni, Kefeng ^a   Zhao, Chengcheng ^a   Ren, Yuhong ^a   Wei, Dongzhi ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Directional immobilization; Formate dehydrogenase; Iron oxide nanoparticles; Polydopamine; Site directed mutagenesis

Indexed keywords

AMINO ACIDS; ENZYME ACTIVITY; METAL NANOPARTICLES;

ACTIVITY OF ENZYMES; CATALYTIC EFFICIENCIES; FORMATE DEHYDROGENASE; IRON OXIDE NANOPARTICLE; POLYDOPAMINE; SITE DIRECTED MUTAGENESIS; SITE-DIRECTED MUTATION; STRUCTURE AND ACTIVITIES;

ENZYME IMMOBILIZATION;

CYSTEINE; FORMATE DEHYDROGENASE; IRON OXIDE; LEUCINE DEHYDROGENASE; NANOPARTICLE; OXIDOREDUCTASE; POLYDOPAMINE COATED IRON OXIDE NANOPARTICLE; UNCLASSIFIED DRUG; FERRIC ION; FERRIC OXIDE; IMMOBILIZED ENZYME; INDOLE DERIVATIVE; METAL NANOPARTICLE; POLYDOPAMINE; POLYMER;

AQUEOUS SOLUTION; ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; COVALENT BOND; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME IMMOBILIZATION; ENZYME STABILITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; LEACHING; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN STRUCTURE; SIMULATION; SITE DIRECTED MUTAGENESIS; TEMPERATURE; WILD TYPE; CHEMISTRY; GENETICS; METABOLISM; SCANNING ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY;

ENZYMES, IMMOBILIZED; FERRIC COMPOUNDS; FORMATE DEHYDROGENASES; INDOLES; METAL NANOPARTICLES; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, ELECTRON, TRANSMISSION; MUTAGENESIS, SITE-DIRECTED; POLYMERS;

EID: 84906737206     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.07.443     Document Type: Article

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