Imaging and quantification of endothelial cell loss in eye bank prepared DMEK grafts using trainable segmentation software

Current Eye Research

Volumn 39, Issue 9, 2014, Pages 894-901

  Jardine, Griffin J ^a   Holiman, Jeffrey D ^b   Stoeger, Christopher G ^b   Chamberlain, Winston D ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^b Lions VisionGift   (United States)

Author keywords

Corneal transplant surgery; Descemet Membrane Endothelial Keratoplasty; Endothelial cell loss; Eye bank; Trainable segmentation

Indexed keywords

ARTICLE; CORNEAL ENDOTHELIAL CELL LOSS; DESCEMET MEMBRANE ENDOTHELIAL KERATOPLASTY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; EYEBANK; HUMAN; HUMAN TISSUE; IMAGE ANALYSIS; IMAGING SOFTWARE; INTERMETHOD COMPARISON; PRIORITY JOURNAL; QUALITY CONTROL; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; SAFETY; SURGICAL TECHNIQUE; ADULT; CELL COUNT; CELL SURVIVAL; COMPUTER PROGRAM; CORNEA ENDOTHELIUM; DESCEMET STRIPPING ENDOTHELIAL KERATOPLASTY; DONOR; IMAGE PROCESSING; LABORATORY DIAGNOSIS; METABOLISM; MIDDLE AGED; PATHOLOGY; PROCEDURES;

CALCEIN AM; FLUORESCEIN DERIVATIVE; FLUORESCENT DYE;

ADULT; CELL COUNT; CELL SURVIVAL; CORNEAL ENDOTHELIAL CELL LOSS; DESCEMET STRIPPING ENDOTHELIAL KERATOPLASTY; ENDOTHELIUM, CORNEAL; EYE BANKS; FLUORESCEINS; FLUORESCENT DYES; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MIDDLE AGED; SOFTWARE; SPECIMEN HANDLING; TISSUE DONORS;

EID: 84906282062     PISSN: 02713683     EISSN: 14602202     Source Type: Journal    
DOI: 10.3109/02713683.2014.887120     Document Type: Article

References (19)

1. Arenas E, Esquenazi S, Anwar M, Terry M. Lamellar corneal transplantation. Surv Ophthalmol 2012;57:510-529.
2. Tillett CW. Posterior lamellar keratoplasty. Am J Ophthalmol 1956;41:530-533.
3. Melles GR, Eggink FA, Lander F, Pels E, Rietveld FJ, Beekhuis WH, et al. A surgical technique for posterior lamellar keratoplasty. Cornea 1998;17:618-626.
4. Price MO, Price Jr FW. Descemet's membrane endothelial keratoplasty surgery: update on the evidence and hurdles to acceptance. Curr Opin Ophthalmol 2013;24: 329-335.
5. Lass JH, Sugar A, Benetz BA, Beck RW, Dontchev M, Gai RL, et al. Endothelial cell density to predict endothelial graft failure after penetrating keratoplasty. Arch Ophthalmol 2010;128:63-69.
6. Price MO, Price Jr FW. Endothelial keratoplasty - a review. Clin Exp Ophthalmol 2010;38:128-140.
7. Saad HA, Terry MA, Shamie N, Chen ES, Friend DF, Holiman JD, et al. An easy and inexpensive method for quantitative analysis of endothelial damage by using vital dye staining and Adobe Photoshop software. Cornea 2008; 27:818-824.
8. Gain P, Thuret G, Chiquet C, Dumollard JM, Mosnier JF, Burillon C, et al. Value of two mortality assessment techniques for organ cultured corneal endothelium: trypan blue versus TUNEL technique. Br J Ophthalmol 2002;86:306-310.
9. Venzano D, Pagani P, Randazzo N, Cabiddu F, Traverso CE. Descemet membrane air-bubble separation in donor corneas. J Cataract Refract Surg 2010;36:2022-2027.
10. McKee HD, Irion LC, Carley FM, Jhanji V, Brahma AK. Donor preparation using pneumatic dissection in endothelial keratoplasty: DMEK or DSEK? Cornea 2012;31: 798-800.
11. Krabcova I, Studeny P, Jirsova K. Endothelial quality of pre-cut posterior corneal lamellae for Descemet's membrane endothelial keratoplasty with a stromal rim (DMEK-S): two-year outcome of manual preparation in an ocular tissue bank. Cell Tissue Bank 2013;14:325-331.
12. Kelliher C, Engler C, Speck C, Ward D, Farazdaghi S, Jun AS. A comprehensive analysis of eye bank-prepared posterior lamellar corneal tissue for use in endothelial keratoplasty. Cornea 2009;28:966-970.
13. Ide T, Yoo SH, Kymionis GD, Goldman JM, Perez VL, O'Brien TP. Descemet-stripping automated endothelial keratoplasty: effect of anterior lamellar corneal tissueon/- off storage condition on Descemet-stripping automated endothelial keratoplasty donor tissue. Cornea 2008; 27:754-757.
14. Terry MA, Saad HA, Shamie N, Chen ES, Phillips PM, Friend DJ, et al. Endothelial keratoplasty: the influence of insertion techniques and incision size on donor endothelial survival. Cornea 2009;28:24-31.
15. Mehta JS, Por YM, Poh R, Beuerman RW, Tan D. Comparison of donor insertion techniques for Descemet stripping automated endothelial keratoplasty. Arch Ophthalmol 2008;126:1383-1388.
16. Guerra FP, Anshu A, Price MO, Giebel AW, Price FW. Descemet's membrane endothelial keratoplasty: prospective study of 1-year visual outcomes, graft survival, and endothelial cell loss. Ophthalmology 2011;118:2368-2373.
17. Schindelin J, Arganda-Carreras I, Frise E, Longair M, Pietzsch T, Preibisch S, et al. Fiji: an open-source platform for biological-image analysis. Nat Methods 2012;9:676-682.
18. Imbert D, Cullander C. Assessment of cornea viability by confocal laser scanning microscopy and MTTassay. Cornea 1997;16:666-674.
19. Bozyczko-Coyne D, McKenna BW, Connors TJ, Neff NT. A rapid fluorometric assay to measure neuronal survival in vitro. J Neurosci Methods 1993;50:205-216.