Multilocus sequence and microsatellite identification of intra-specific hybrids and ancestor-like donors among natural ethiopian isolates of leishmania donovani

International Journal for Parasitology

Volumn 44, Issue 10, 2014, Pages 751-757

  Gelanew, Tesfaye ^a,d   Hailu, Asrat ^b   Scho'nian, Gabriele ^c   Lewis, Michael D ^d   Miles, Michael A ^d   Yeo, Matthew ^d  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^b ADDIS ABABA UNIVERSITY   (Ethiopia)

^c CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^d LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

Author keywords

Genetic exchange; Hybridisation; Leishmania; MLMT; MLST; Recombination

Indexed keywords

CYTOCHROME B; KINETOPLAST DNA; MICROSATELLITE DNA; MITOCHONDRIAL DNA;

ALLELE; CLONE; DNA; GENE; GENOTYPE; HYBRIDIZATION; LEISHMANIASIS; MITOCHONDRION; PARASITE; PROTOZOAN; RECOMBINATION;

ARTICLE; CYTOCHROME B GENE; DIPLOIDY; DNA CONTENT; ETHIOPIA; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; GENE SEQUENCE; HYBRID; LEISHMANIA DONOVANI; MICROSATELLITE MARKER; MOLECULAR TYPING; MULTILOCUS MICROSATELLITE TYPING; MULTILOCUS SEQUENCE TYPING; NATURAL POPULATION; NONHUMAN; NUCLEOTIDE SEQUENCE; ANIMAL; EVOLUTION; GENETICS; GENOTYPE; HUMAN; LEISHMANIASIS, VISCERAL; PARASITOLOGY;

ETHIOPIA;

KINETOPLASTIDA; LEISHMANIA DONOVANI; PROTOZOA; TRYPANOSOMATIDAE;

ANIMALS; BIOLOGICAL EVOLUTION; DNA, MITOCHONDRIAL; ETHIOPIA; GENOTYPE; HUMANS; LEISHMANIA DONOVANI; LEISHMANIASIS, VISCERAL; MICROSATELLITE REPEATS; MULTILOCUS SEQUENCE TYPING;

EID: 84906068458     PISSN: 00207519     EISSN: 18790135     Source Type: Journal    
DOI: 10.1016/j.ijpara.2014.05.008     Document Type: Article

References (25)

1. Akopyants N.S., Kimblin N., Secundino N., Patrick R., Peters N., Lawyer P., Dobson D.E., Beverley S.M., Sacks D.L. Demonstration of genetic exchange during cyclical development of Leishmania in the sand fly vector. Science 2009, 324:265-268.
2. Banuls A.L., Guerrini F., Le Pont F., Barrera C., Espinel I., Guderian R., Echeverria R., Tibayrenc M. Evidence for hybridization by multilocus enzyme electrophoresis and random amplified polymorphic DNA between Leishmania braziliensis and Leishmania panamensis/guyanensis in Ecuador. J. Eukaryot. Microbiol. 1997, 44:408-411.
3. Belli A.A., Miles M.A., Kelly J.M. A putative Leishmania panamensis/Leishmania braziliensis hybrid is a causative agent of human cutaneous leishmaniasis in Nicaragua. Parasitology 1994, 109:435-442.
4. Chargui N., Amro A., Haouas N., Schonian G., Babba H., Schmidt S., Ravel C., Lefebvre M., Bastien P., Chaker E., Aoun K., Zribi M., Kuhls K. Population structure of Tunisian Leishmania infantum and evidence for the existence of hybrids and gene flow between genetically different populations. Int. J. Parasitol. 2009, 39:801-811.
5. Downing T., Imamura H., Decuypere S., Clark T.G., Coombs G.H., Cotton J.A., Hilley J.D., de Doncker S., Maes I., Mottram J.C., Quail M.A., Rijal S., Sanders M., Schonian G., Stark O., Sundar S., Vanaerschot M., Hertz-Fowler C., Dujardin J.C., Berriman M. Whole genome sequencing of multiple Leishmania donovani clinical isolates provides insights into population structure and mechanisms of drug resistance. Genome Res. 2011, 21:2143-2156.
6. Gelanew T., Kuhls K., Hurissa Z., Weldegebreal T., Hailu W., Kassahun A., Abebe T., Hailu A., Schonian G. Inference of population structure of Leishmania donovani strains isolated from different Ethiopian visceral leishmaniasis endemic areas. PLoS Negl. Trop. Dis. 2010, 4:e889.
7. Hamad S.H., Musa A.M., Khalil E.A.G., Abebe T., Younis B.M. Leishmania: probable genetic hybrids between species in Sudanese isolates. J. Microbiol. Antimicrob. 2011, 3:142-145.
8. Inbar E., Akopyants N.S., Charmoy M., Romano A., Lawyer P., Elnaiem D.E., Kauffmann F., Barhoumi M., Grigg M., Owens K., Fay M., Dobson D.E., Shaik J., Beverley S.M., Sacks D. The mating competence of geographically diverse Leishmania major strains in their natural and unnatural sand fly vectors. PLoS Genet. 2013, 9:e1003672.
9. Kelly J.M., Law J.M., Chapman C.J., Van Eys G.J., Evans D.A. Evidence of genetic recombination in Leishmania. Mol. Biochem. Parasitol. 1991, 46:253-263.
10. Kuhls K., Keilonat L., Ochsenreither S., Schaar M., Schweynoch C., Presber W., Schonian G. Multilocus microsatellite typing (MLMT) reveals genetically isolated populations between and within the main endemic regions of visceral leishmaniasis. Microbes Infect. 2007, 9:334-343.
11. Luyo-Acero G.E., Uezato H., Oshiro M., Takei K., Kariya K., Katakura K., Gomez-Landires E., Hashiguchi Y., Nonaka S. Sequence variation of the cytochrome b gene of various human infecting members of the genus Leishmania and their phylogeny. Parasitology 2004, 128:483-491.
12. Mauricio I.L., Stothard J.R., Miles M.A. Leishmania donovani complex: genotyping with the ribosomal internal transcribed spacer and the mini-exon. Parasitology 2004, 128:263-267.
13. Miles M.A., Yeo M., Mauricio I.L. Genetics. Leishmania exploit sex. Science 2009, 324:187-189.
14. Nolder D., Roncal N., Davies C.R., Llanos-Cuentas A., Miles M.A. Multiple hybrid genotypes of Leishmania (viannia) in a focus of mucocutaneous Leishmaniasis. Am. J. Trop. Med. Hyg. 2007, 76:573-578.
15. Ochsenreither S., Kuhls K., Schaar M., Presber W., Schonian G. Multilocus microsatellite typing as a new tool for discrimination of Leishmania infantum MON-1 strains. J. Clin. Microbiol. 2006, 44:495-503.
16. Odiwuor S., De Doncker S., Maes I., Dujardin J.C., Van der Auwera G. Natural Leishmania donovani/Leishmania aethiopica hybrids identified from Ethiopia. Infection, genetics and evolution. J. Mol. Epidemiol. Evol. Genet. Infect. Dis. 2011, 11:2113-2118.
17. Ravel C., Cortes S., Pratlong F., Morio F., Dedet J.P., Campino L. First report of genetic hybrids between two very divergent Leishmania species: Leishmania infantum and Leishmania major. Int. J. Parasitol. 2006, 36:1383-1388.
18. Rogers M.B., Downing T., Smith B.A., Imamura H., Sanders M., Svobodova M., Volf P., Berriman M., Cotton J.A., Smith D.F. Genomic confirmation of hybridisation and recent inbreeding in a vector-isolated Leishmania population. PLoS Genet. 2014, 10:e1004092.
19. Rougeron V., Banuls A.L., Carme B., Simon S., Couppie P., Nacher M., Hide M., De Meeus T. Reproductive strategies and population structure in Leishmania: substantial amount of sex in Leishmania Viannia guyanensis. Mol. Ecol. 2011, 20:3116-3127.
20. Sadlova J., Yeo M., Seblova V., Lewis M.D., Mauricio I., Volf P., Miles M.A. Visualisation of Leishmania donovani fluorescent hybrids during early stage development in the sand fly vector. PLoS One 2011, 6:e19851.
21. Tibayrenc M., Ayala F.J. The clonal theory of parasitic protozoa: 12 years on. Trends Parasitol. 2002, 18:405-410.
22. Tibayrenc M., Ben Abderrazak S., Guerrini F., Banuls A. Leishmania and the clonal theory of parasitic protozoa. Arch. Inst. Pasteur Tunis 1993, 70:375-382.
23. Volf P., Benkova I., Myskova J., Sadlova J., Campino L., Ravel C. Increased transmission potential of Leishmania major/Leishmania infantum hybrids. Int. J. Parasitol. 2007, 37:589-593.
24. WHO, 2010. Control of Leishmaniases. WHO Technical Report Series, 949.
25. Yeo M., Lewis M.D., Carrasco H.J., Acosta N., Llewellyn M., da Silva Valente S.A., de Costa Valente V., de Arias A.R., Miles M.A. Resolution of multiclonal infections of Trypanosoma cruzi from naturally infected triatomine bugs and from experimentally infected mice by direct plating on a sensitive solid medium. Int. J. Parasitol. 2007, 37:111-120.