A simplified chromatographic approach to purify commercially available bovine submaxillary mucins (BSM)

Preparative Biochemistry and Biotechnology

Volumn 45, Issue 1, 2015, Pages 84-99

  Madsen, Jan Busk ^a   Pakkanen, Kirsi I ^a   Duelund, Lars ^b   Svensson, Birte ^a   Hachem, Maher Abou ^a   Lee, Seunghwan ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

Author keywords

anion exchange chromatography (AEC); bovine serum albumin (BSA); bovine submaxillary mucin (BSM); mucin; purification

Indexed keywords

BOVINE SERUM ALBUMIN; BSM1 PROTEIN, BOS TAURUS; MUCIN;

ANIMAL; BOVINE; CHEMISTRY; CIRCULAR DICHROISM; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCEDURES; THIN LAYER CHROMATOGRAPHY;

ANIMALS; CATTLE; CHROMATOGRAPHY, ION EXCHANGE; CHROMATOGRAPHY, THIN LAYER; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MUCINS; SERUM ALBUMIN, BOVINE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84906050587     PISSN: 10826068     EISSN: 15322297     Source Type: Journal    
DOI: 10.1080/10826068.2014.887583     Document Type: Article

References (64)

1. Desseyn, J.-L.; Tetaert, D.; Gouyer, V. Architecture of the Large Membrane-Bound Mucins. Gene 2008, 410, 215-222.
2. Hattrup, C.L.; Gendler, S.J. Structure and Function of the Cell Surface (Tethered) Mucins. Annu. Rev. Physiol. 2008, 70, 431-457.
3. Linden, S.K.; Sutton, P.; Karlsson, N.G.; Korolik, V.; McGuckin, M.A. Mucins in the Mucosal Barrier to Infection. Mucosal Immunol. 2008, 1, 183-197.
4. Thornton, D.J.; Rousseau, K.; McGuckin, M.A. Structure and Function of the Polymeric Mucins in Airways Mucus. Annu. Rev. Physiol. 2008, 70, 459-486.
5. Hoorens, P.; Rinaldi, M.; Li, R.; Goddeeris, B.; Claerebout, E.; Vercruysse, J.; Geldhof, P. Genome Wide Analysis of the Bovine Mucin Genes and Their Gastrointestinal Transcription Profile. BMC Genomics 2011, 12, 140.
6. Tian, E.; Ten Hagen, K.G. Recent Insights Into the Biological Roles of Mucin-Type O-Glycosylation. Glycoconjugate J. 2009, 26, 325-334.
7. Hong, Z.; Chasan, B.; Bansil, R.; Turner, B.S.; Bhaskar, K.R.; Afdhal, N.H. Atomic Force Microscopy Reveals Aggregation of Gastric Mucin at Low pH. Biomacromolecules 2005, 6, 3458-3466.
8. Landry, R.M.; An, D.; Hupp, J.T.; Singh, P.K.; Parsek, M.R. Mucin-Pseudomonas aeruginosa Interactions Promote Biofilm Formation and Antibiotic Resistance. Mol. Microbiol. 2006, 59, 142-151.
9. Berry, M.; McMaster, T.J.; Corfield, A.P.; Miles, M.J. Exploring the Molecular Adhesion of Ocular Mucins. Biomacromolecules 2001, 2, 498-503.
10. Liao, X.; Wiedmann, T. Formation of Cholesterol Crystals at a Mucin Coated Substrate. Pharm. Res. 2006, 23, 2413-2416.
11. Bushnak, I.A.; Labeed, F.H.; Sear, R.P.; Keddie, J.L. Adhesion of Microorganisms to Bovine Submaxillary Mucin Coatings: Effect of Coating Deposition Conditions. Biofouling 2010, 26, 387-397.
12. Joergensen, L.; Klösgen, B.; Simonsen, A.C.; Borch, J.; Hagesaether, E. New Insights Into the Mucoadhesion of Pectins by AFM Roughness Parameters in Combination With SPR. Int. J. Pharm. 2011, 411, 162-168.
13. Svensson, O.; Thuresson, K.; Arnebrant, T. Interactions Between Chitosan-Modified Particles and Mucin-Coated Surfaces. J. Colloid Interface Sci. 2008, 325, 346-350.
14. Dague, E.; Le, D.T.L.; Zanna, S.; Marcus, P.; Loubière, P.; Mercier-Bonin, M. Probing In Vitro Interactions Between Lactococcus lactis and Mucins Using AFM. Langmuir 2010, 26, 11010-11017.
15. Dedinaite, A.; Lundin, M.; Macakova, L.; Auletta, T. Mucin Chitosan Complexes at the Solid Liquid Interface: Multilayer Formation and Stability in Surfactant Solutions. Langmuir 2005, 21, 9502-9509.
16. Feldoto, Z.; Pettersson, T.; Dedinaite, A. Mucin Electrolyte Interactions at the Solid Liquid Interface Probed by QCM-D. Langmuir 2008, 24, 3348-3357.
17. Su, Y.; Xu, Y.; Yang, L.; Weng, S.; Soloway, R.D.; Wang, D.; Wu, J. Spectroscopic Studies of the Effect of the Metal Ions on the Structure of Mucin. J. Mol. Struct. 2009, 920, 8-13.
18. Iijima, M.; Yoshimura, M.; Tsuchiya, T.; Tsukada, M.; Ichikawa, H.; Fukumori, Y.; Kamiya, H. Direct Measurement of Interactions Between Stimulation-Responsive Drug Delivery Vehicles and Artificial Mucin Layers by Colloid Probe Atomic Force Microscopy. Langmuir 2008, 24, 3987-3992.
19. Sriamornsak, P.; Wattanakorn, N.; Takeuchi, H. Study on the Mucoadhesion Mechanism of Pectin by Atomic Force Microscopy and Mucin-Particle Method. Carbohydr. Polym. 2010, 79, 54-59.
20. Kocevar-Nared, J.; Kristl, J.; Smid-Korbar, J. Comparative Rheological Investigation of Crude Gastric Mucin and Natural Gastric Mucus. Biomaterials 1997, 18, 677-681.
21. Raynal, B.D.E.; Hardingham, T.E.; Thornton, D.J.; Sheehan, J.K. Concentrated Solutions of Salivary MUC5B Mucin Do Not Replicate the Gel-Forming Properties of Saliva. Biochem. J. 2002, 362, 289-296.
22. Sandberg, T.; Blom, H.; Caldwell, K.D. Potential Use of Mucins as Biomaterial Coatings. I. Fractionation, Characterization, and Model Adsorption of Bovine, Porcine, and Human Mucins. J. Biomed. Mater. Res. Part A 2009, 91A, 762-772.
23. Sandberg, T.; Mellin, L.; Gelius, U.; Caldwell, K.D. Surface Analysis of Pure and Complex Mucin Coatings on a Real-Type Substrate Using Individual and Combined mBCA, ELLA, and ELISA. J. Colloid Interface Sci. 2009, 333, 180-187.
24. Sandberg, T.; Karlsson Ott, M.; Carlsson, J.; Feiler, A.; Caldwell, K.D. Potential Use of Mucins as Biomaterial Coatings. II. Mucin Coatings Affect the Conformation and Neutrophil-Activating Properties of Adsorbed Host Proteins-Toward a Mucosal Mimic. J. Biomed. Mater. Res. Part A 2009, 91A, 773-785.
25. Sandberg, T.; Carlsson, J.; Ott, M.K. Mucin Coatings Suppress Neutrophil Adhesion to a Polymeric Model Biomaterial. Microsc. Res. Tech. 2007, 70, 864-868.
26. Malmsten, M.; Blomberg, E.; Claesson, P.; Carlstedt, I.; Ljusegren, I. Mucin Layers on Hydrophobic Surfaces Studied With Ellipsometry and Surface Force Measurements. J. Colloid Interface Sc. 1992, 151, 579-590.
27. Bligh, E.G.; Dyer, W.J. A Rapid Method of Total Lipid Extraction and Purification. Can. J. Biochem. Physiol. 1959, 37, 911-917.
28. Zhang, X.; Wang, Q.; Lou, X.; Sun, H.; Roepstorff, P.; Liu, S. An On-Target Desalting and Concentration Sample Preparation Protocol for MALDI-MS and MS=MS Analysis. In Liver Proteomics, Josic, D., Hixson, D.C., Eds.; Humana Press: Totowa, NJ, 2012; pp. 17-28.
29. Yakubov, G.E.; Papagiannopoulos, A.; Rat, E.; Easton, R.L.; Waigh, T.A. Molecular Structure and Rheological Properties of Short-Side-Chain Heavily Glycosylated Porcine Stomach Mucin. Biomacromolecules 2007, 8, 3467-3477.
30. Yakubov, G.E.; Papagiannopoulos, A.; Rat, E.; Waigh, T.A. Charge and Interfacial Behavior of Short Side-Chain Heavily Glycosylated Porcine Stomach Mucin. Biomacromolecules 2007, 8, 3791-3799.
31. Lundin, M.; Sandberg, T.; Caldwell, K.D.; Blomberg, E. Comparison of the Adsorption Kinetics and Surface Arrangement of "As Received" and Purified Bovine Submaxillary Gland Mucin (BSM) on Hydrophilic Surfaces. J. Colloid Interface Sci. 2009, 336, 30-39.
32. List, S.J.; Findlay, B.P.; Forstner, G.G.; Forstner, J.F. Enhancement of the Viscosity of Mucin by Serum Albumin. Biochem. J. 1978, 175, 565-571.
33. Feiler, A.A.; Sahlholm, A.; Sandberg, T.; Caldwell, K.D. Adsorption and Viscoelastic Properties of Fractionated Mucin (BSM) and Bovine Serum Albumin (BSA) Studied With Quartz Crystal Microbalance (QCM-D). J. Colloid Interface Sci. 2007, 315, 475-481.
34. Perez, E.; Proust, J.E. Forces Between Mica Surfaces Covered With Adsorbed Mucin Across Aqueous Solution. J. Colloid Interface Sci. 1987, 118, 182-191.
35. Yao, J.K.; Rastetter, G.M. Microanalysis of Complex Tissue Lipids by High-Performance Thin-Layer Chromatography. Anal. Biochem. 1985, 150, 111-116.
36. Eftink, M.R.; Ghiron, C.A. Fluorescence Quenching Studies With Proteins. Anal. Biochem. 1981, 114, 199-227.
37. Lakowicz, J.R. Principles of Fluorescence Spectroscopy. 3rd ed.; Springer Science\+Business Media: New York, NY, 2006.
38. Ivanov, A.E.; Solodukhina, N.; Wahlgren, M.; Nilsson, L.; Vikhrov, A.A.; Nikitin, M.P.; Orlov, A.V.; Nikitin, P.I.; Kuzimenkova, M.V.; Zubov, V.P. Reversible Conformational Transitions of a Polymer Brush Containing Boronic Acid and Its Interaction With Mucin Glycoprotein. Macromol. Biosci. 2011, 11, 275-284.
39. Jeffers, F.; Fuell, C.; Tailford, L.E.; MacKenzie, D.A.; Bongaerts, R.J.; Juge, N. Mucin-Lectin Interactions Assessed by Flow Cytometry. Carbohydr. Res. 2010, 345, 1486-1491.
40. Yang, J.T.; Wu, C.S.C.; Böhm, G. 7 Circular Dichroism (CD) Parameters and Secondary Structure Estimates of Proteins. In Structural and Physical Data I. Hinz, H.J., Ed.; Springer: Berlin, Germany, 2003; pp. 7001-7018.
41. Pigman, W.; Moschera, J.; Weiss, M.; Tettamanti, G. The Occurrence of Repetitive Glycopeptide Sequences in Bovine Submaxillary Glycoprotein. Eur. J. Biochem. 1973, 32, 148-154.
42. Dekker, J.; Rossen, J.W.A.; Büller, H.A.; Einerhand, A.W.C. The MUC Family: An Obituary. Trends Biochem. Sci. 2002, 27, 126-131.
43. Venyaminov, S.Y.; Vassilenko, K.S. Determination of Protein Tertiary Structure Class From Circular Dichroism Spectra. Anal. Biochem. 1994, 222, 176-184.
44. Bhargava, A.K.;Woitach, J.T.; Davidson, E.A.; Bhavanandan, V.P. Cloning and cDNA Sequence of a Bovine Submaxillary Gland Mucin-Like Protein Containing Two Distinct Domains. Proc. Natl. Acad. Sci. USA 1990, 87, 6798-6802.
45. Kamat, B.P.; Seetharamappa, J. In Vitro Study on the Interaction of Mechanism of Tricyclic Compounds With Bovine Serum Albumin. J. Pharm. Biomed. Anal. 2004, 35, 655-664.
46. Zhang, Y.-Z.; Zhang, X.-P.; Hou, H.-N.; Dai, J.; Liu, Y. Study on the Interaction Between Cu2\+ and Bovine Serum Albumin by Spectroscopic Methods. Biol. Trace Elem. Res. 2008, 121, 276-287.
47. Rogozea, A.; Matei, I.; Turcu, I.M.; Ionita, G.; Sahini, V.E.; Salifoglou, A. EPR and Circular Dichroism Solution Studies on the Interactions of Bovine Serum Albumin With Ionic Surfactants and b-Cyclodextrin. J. Phys. Chem. B 2012, 116, 14245-14253.
48. Moriyama, Y.; Ohta, D.; Hachiya, K.; Mitsui, Y.; Takeda, K. Fluorescence Behavior of Tryptophan Residues of Bovine and Human Serum Albumins in Ionic Surfactant Solutions: A Comparative Study of the Two and One Tryptophan(s) of Bovine and Human Albumins. J. Protein Chem. 1996, 15, 265-272.
49. Jiang, W.; Woitach, J.T.; Gupta, D.; Bhavanandan, V.P. Sequence of a Second Gene Encoding Bovine Submaxillary Mucin: Implication for Mucin Heterogeneity and Cloning. Biochem. Biophys. Res. Commun. 1998, 251, 550-556.
50. Cordeiro, Y.M.F.; Juliano, L.; Juliano, M.A.; Brentani, R.R.; Foguel, D.; Silva, J.L. DNA Converts Cellular Prion Protein Into the Beta-Sheet Conformation and Inhibits Prion Peptide Aggregation. J. Biol. Chem. 2001, 276, 49400-49409.
51. Hedge, M.S.B.; Hedge, P.M.; Bellot L.J.; Hazra, T.K.; Boldogh, I.; Mitra, S. Enhancement of NEIL1 Protein-Initiated Oxidized DNA Base Excision Repair by Heterogeneous Nuclear Ribonucleoprotein U (hnRNP-U) via Direct Interaction. J. Biol. Chem. 2012, 287, 34202-34211.
52. Barrera, F.N.; Fendos, J.; Engelman, D.M. Membrane Physical Properties Influence Transmembrane Helix Formation. Proc. Natl. Acad. Sci. USA 2012, 109, 14422-14427.
53. Falanga, A.; Tarallo, R.; Vitiello, G.; Vitiello, M.; Perillo, E.; Cantisani, M.; D'Errico, G.; Galdiero, M.; Galdiero, S. Biophysical Characterization and Membrane Interaction of the Two Fusion Loops of Glycoprotein b From Herpes Simplex Type I Virus. PLoS One 2012, 7, e32186.
54. Agudelo, D.; Bourassa, P.; Bruneau, J.; Bérubé, G.; Asselin, É .; Tajmir-Riahi, H.-A. Probing the Binding Sites of Antibiotic Drugs Doxorubicin and N-(Trifluoroacetyl) Doxorubicin With Human and Bovine Serum Albumins. PLoS One 2012, 7, e43814.
55. Hung, H.-C.; Liu, C.-L.; Hsu, J.T.A.; Horng, J.-T.; Fang, M.-Y.; Wu, S.-Y.; Ueng, S.-H.; Wang, M.-Y.; Yaw, C.-W.; Hou, M.-H. Development of an Anti-Influenza Drug Screening Assay Targeting Nucleoproteins With Tryptophan Fluorescence Quenching. Anal. Chem. 2012, 84, 6391-6399.
56. Jonas, A.; Weber, G. Presence of Arginine Residues at the Strong, Hydrophobic Anion Binding Sites of Bovine Serum Albumin. Biochemistry 1971, 10, 1335-1339.
57. Burstein, E.A.; Vedenkina, N.S.; Ivkova, M.N. Fluorescence and the Location of Tryptophan Residues in Protein Molecules. Photochem. Photobiol. 1973, 18, 263-279.
58. Burstein, E.A.; Vedenkina, N.S.; Ivkova, M.N. Fluorescence and the Location of Tryptophan Residues in Protein Molecules. Photochem. Photobiol. 1973, 18, 263-279.
59. Strous, G.J.; Dekker, J. Mucin-Type Glycoproteins. Crit. Rev. Biochem. Mol. Biol. 1992, 27, 57-92.
60. White, A. Effect of pH on Fluorescence of Tryosine, Tryptophan and Related Compounds. Biochem. J. 1959, 71, 217-220.
61. Yu, H.T.; Colucci, W.J.; McLaughlin, M.L.; Barkley, M.D. Fluorescence Quenching in Indoles by Excited-State Proton Transfer. J. Am. Chem. Soc. 1992, 114, 8449-8454.
62. Bardik, V.; Gotsulskii, V.; Pavlov, E.; Malomuzh, N.; Nerukh, D.; Yanchuk, I.; Lavoryk, S. Light Scattering Study of Human Serum Albumin in Pre-Denaturation: Relation to Dynamic Transition in Water at 42-C. J. Mol. Liquids 2012, 176, 60-64.
63. Karahan, M.; Mustafaeva, Z.; Ö zeroglu, C. Investigation of Ternary Complex Formations of Polyacrylic Acid With Bovine Serum Albumin in the Presence of Metal Ions by Fluorescence and Dynamic Light Scattering Measurements. Protein J. 2010, 29, 336-342.
64. Luik, A.I.; Naboka, Y.N.; Mogilevich, S.E.; Hushcha, T.O.; Mischenko, N.I. Study of Human Serum Albumin Structure by Dynamic Light Scattering: Two Types of Reactions Under Different pH and Interaction With Physiologically Active Compounds. Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 1998, 54, 1503-1507.