A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification

BioTechniques

Volumn 57, Issue 2, 2014, Pages 81-87

  Ignatov, Konstantin B ^a,b   Barsova, Ekaterina V ^c,d   Fradkov, Arkady F ^c,d   Blagodatskikh, Konstantin A ^b,e   Kramarova, Tatiana V ^f   Kramarov, Vladimir M ^a,b  

^a VAVILOV INSTITUTE OF GENERAL GENETICS   (Russian Federation)

^b ALL RUSSIA RESEARCH INSTITUTE OF AGRICULTURAL BIOTECHNOLOGY   (Russian Federation)

^c SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^d Evrogen JSC   (Russian Federation)

^e Syntol Ltd   (Russian Federation)

^f STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

DNA polymerase; Isothermal amplification; LAMP; PCDR; PCR; Quantitative amplification; Real time amplification; Strand displacement

Indexed keywords

DNA; PRIMER DNA; TAQ POLYMERASE;

ANIMAL; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; MOUSE; MYCOBACTERIUM TUBERCULOSIS; NUCLEIC ACID AMPLIFICATION; POLYMERASE CHAIN REACTION; PROCEDURES; SENSITIVITY AND SPECIFICITY;

ANIMALS; DNA; DNA PRIMERS; MICE; MYCOBACTERIUM TUBERCULOSIS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TAQ POLYMERASE;

EID: 84905859027     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000114198     Document Type: Article

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