Imaging mGluR5 dynamics in astrocytes using quantum dots

Current Protocols in Neuroscience

Volumn , Issue SUPPL.66, 2014, Pages

  Arizono, Misa ^a,b   Bannai, Hiroko ^a,c   Mikoshiba, Katsuhiko ^a  

^a Brain Science Institute   (Japan)

^b University of Bordeaux   (France)

^c NAGOYA UNIVERSITY   (Japan)

Author keywords

Astrocyte; mGluR5; Quantum dot; Single particle tracking

Indexed keywords

CYAN FLUORESCENT PROTEIN; MEMBRANE PROTEIN; MEMBRANE RECEPTOR; METABOTROPIC RECEPTOR 5; NEUROTRANSMITTER RECEPTOR; POLYMER; QUANTUM DOT;

ANIMAL CELL; ARTICLE; ASTROCYTE; BRAIN BLOOD FLOW; BRAIN METABOLISM; CALCIUM CELL LEVEL; CALCIUM SIGNALING; CELL COMMUNICATION; CELL CULTURE; CELL MEMBRANE; CELL STRUCTURE; CONTROLLED STUDY; CULTURE MEDIUM; CYTOSOL; DIFFUSION; DIFFUSION COEFFICIENT; FEMALE; GLIA CELL; HIPPOCAMPUS; ILLUMINATION; NERVE CELL PLASTICITY; NEUROTRANSMISSION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SENSORY STIMULATION; SIGNAL NOISE RATIO; SYNAPTIC TRANSMISSION;

EID: 84905695419     PISSN: 19348584     EISSN: 19348576     Source Type: Journal    
DOI: 10.1002/0471142301.ns0221s66     Document Type: Article

References (19)

1. Arizono, M., Bannai, H., Nakamura, K., Niwa, F., Enomoto, M., Matsu-Ura, T., Miyamoto, A., Sherwood,M.W., Nakamura, T., and Mikoshiba, K. 2012. Receptor-selective diffusion barrier enhances sensitivity of astrocytic processes to metabotropic glutamate receptor stimulation. Sci. Signal. 5:ra27.
2. Bannai, H., Levi, S., Schweizer, C., Dahan,M., and Triller, A. 2006. Imaging the lateral diffusion of membrane molecules with quantum dots. Nat. Protoc. 1:2628-2634.
3. Bannai, H., Levi, S., Schweizer, C., Inoue, T., Launey, T.,Racine,V., Sibarita, J.B., Mikoshiba, K., and Triller, A. 2009. Activity-dependent tuning of inhibitory neurotransmission based on GABAAR diffusion dynamics. Neuron 62:670-682.
4. Charles, A.C., Merrill, J.E., Dirksen, E.R., and Sanderson, M.J. 1991. Intercellular signaling in glial cells: Calcium waves and oscillations in response to mechanical stimulation and glutamate. Neuron 6:983-992.
5. Ding, S., Fellin, T., Zhu, Y., Lee, S.Y., Auberson, Y.P., Meaney, D.F., Coulter, D.A., Carmignoto, G., and Haydon, P.G. 2007. Enhanced astrocytic Ca2+ signals contribute to neuronal excitotoxicity after status epilepticus. J. Neurosci. 27:10674-10684.
6. Goslin, K., Asmussen, H., and Banker, G. 1998. Rat hippocampal neurons in low-density culture. In Culturing Nerve Cells (G. Banker and K. Goslin, eds.), pp. 339-370. MIT Press, Cambridge, Mass.
7. Kusumi, A., Sako, Y., and Yamamoto, M. 1993. Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calciuminduced differentiation in cultured epithelial cells. Biophys. J. 65:2021-2040.
8. Lidke, D.S., Nagy, P., and Arndt-Jovin, D.J. 2007. In vivo imaging using quantum-dot-conjugated probes. Curr. Protoc. Cell Biol. 36:25.1.1-25.1.18.
9. Magistretti, P.J. 2006. Neuron-glia metabolic coupling and plasticity. J. Exp. Biol. 209:2304-2311.
10. Muir, J., Arancibia-Carcamo, I.L.,MacAskill, A.F., Smith, K.R., Griffin, L.D., and Kittler, J.T. 2010.NMDAreceptors regulateGABAAreceptor lateral mobility and clustering at inhibitory synapses through serine 327 on the gamma2 subunit. Proc. Natl. Acad. Sci. U.S.A. 107:16679-16684.
11. Nakada, C., Ritchie, K., Oba, Y., Nakamura, M., Hotta, Y., Iino, R., Kasai, R.S., Yamaguchi, K., Fujiwara, T., and Kusumi, A. 2003. Accumulation of anchored proteins formsmembrane diffusion barriers during neuronal polarization. Nat. Cell Biol. 5:626-632.
12. Newman, E.A. 2003. Newroles for astrocytes: Regulation of synaptic transmission. Trends Neurosci. 26:536-542.
13. Niwa, F., Bannai, H., Arizono, M., Fukatsu, K., Triller, A., and Mikoshiba, K. 2012. Gephyrin-independent GABA(A)R mobility and clustering during plasticity. PLoS One 7: e36148.
14. Panatier, A., Vallee, J., Haber, M., Murai, K.K., Lacaille, J.C., and Robitaille, R. 2011. Astrocytes are endogenous regulators of basal transmission at central synapses. Cell 146:785-798.
15. Perea, G. and Araque, A. 2007. Astrocytes potentiate transmitter release at single hippocampal synapses. Science 317:1083-1086.
16. Saxton, M.J. and Jacobson, K. 1997. Single-particle tracking: Applications to membrane dynamics. Annu. Rev. Biophys. Biomol. Struct. 26: 373-399.
17. Simard, M. and Nedergaard, M. 2004. The neurobiology of glia in the context of water and ion homeostasis. Neuroscience 129:877-896.
18. Triller, A. and Choquet, D. 2008. New concepts in synaptic biology derived from single-molecule imaging. Neuron 59:359-374. Viviani, B. 2006. Preparation and coculture of neurons and glial cells. Curr. Protoc. Cell Biol. 32:2.7.1-2.7.21.
19. Viviani, B. 2006. Preparation and coculture of neurons and glial cells. Curr. Protoc. Cell Biol. 32:2.7.1-2.7.21