Real-Time PCR

Current Protocols in Essential Laboratory Techniques

Volumn 2014, Issue , 2014, Pages 10.3.1-10.3.40

  Fraga, Dean ^a   Meulia, Tea ^b   Fenster, Steven ^c  

^a 931 College Mall   (United States)

^b OHIO STATE UNIVERSITY   (United States)

^c FORT LEWIS COLLEGE   (United States)

Author keywords

cDNA; PCR; PCR primer design; Quantifying gene expression; Real time PCR (polymerase chain reaction); SYBR Green; Taq polymerase

Indexed keywords

CHLOROFORM; COMPLEMENTARY DNA; DOUBLE STRANDED DNA; ISOTHIOCYANIC ACID; MAGNESIUM CHLORIDE; MESSENGER RNA; PHENOL; RIBONUCLEASE H; RNA 18S; RNA 28S;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; AGAR GEL ELECTROPHORESIS; ANNEALING TEMPERATURE; ARTICLE; AVIAN MYELOBLASTOSIS VIRUS; CHEMICAL REACTION KINETICS; CONCENTRATION (PARAMETERS); COPY NUMBER VARIATION; CROSS HYBRIDIZATION; DNA SEQUENCE; DNA SYNTHESIS; ENZYME ACTIVITY; FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; GEL ELECTROPHORESIS; GENE DUPLICATION; GENE EXPRESSION; LIMIT OF DETECTION; LIMIT OF QUANTITATION; MEASUREMENT ACCURACY; MELTING POINT; MURINE LEUKEMIA VIRUS; NONHUMAN; POLYMERASE CHAIN REACTION SYSTEM; PRIORITY JOURNAL; PROCESS OPTIMIZATION; QUALITY CONTROL; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; RNA ISOLATION; RNA PURIFICATION; STANDARDIZATION; TEMPERATURE MEASUREMENT; VIRUS STRAIN;

EID: 84905389501     PISSN: None     EISSN: 19483430     Source Type: Journal    
DOI: 10.1002/9780470089941.et1003s08     Document Type: Article

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