Isolation and partial characterization of a new human glioblastoma cell line

Chirurgia (Romania)

Volumn 104, Issue 4, 2009, Pages 453-461

  Brehar, F M ^a   Bleotu, C ^b   Stefan, L M ^c   Buzgariu, W ^c   Chivu, M ^b   Utoiu, E ^c   Matei, L ^b   Ciurea, A V ^a   Tascu, A ^a  

^a Clinic Hospital Bagdasar Arseni Bucharest   (Romania)

^b STEFAN S NICOLAU INSTITUTE OF VIROLOGY   (Romania)

^c National Institute of Research and Development for Biological Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABCB1 PROTEIN, HUMAN; ACTIN; CD133 ANTIGEN; EPIDERMAL GROWTH FACTOR RECEPTOR; GLIAL FIBRILLARY ACIDIC PROTEIN; GLYCOPROTEIN; GLYCOPROTEIN P; INTERMEDIATE FILAMENT PROTEIN; LEUKOCYTE ANTIGEN; NERVE PROTEIN; NESTIN; PEPTIDE; PROTEIN BCL 2; TUMOR MARKER; VASCULOTROPIN A;

ANIMAL; ARTICLE; BRAIN TUMOR; CANCER STEM CELL; CHEMISTRY; COMPARATIVE STUDY; CULTURE TECHNIQUE; DISEASE MODEL; FEMALE; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; GLIOBLASTOMA; HUMAN; IN VITRO STUDY; METABOLISM; MOUSE; PATHOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSPLANTATION; TUMOR CELL LINE; WESTERN BLOTTING; XENOGRAFT;

ACTINS; ANIMALS; ANTIGENS, CD; BLOTTING, WESTERN; BRAIN NEOPLASMS; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; DISEASE MODELS, ANIMAL; FEMALE; FLOW CYTOMETRY; GENE EXPRESSION REGULATION, NEOPLASTIC; GLIAL FIBRILLARY ACIDIC PROTEIN; GLIOBLASTOMA; GLYCOPROTEINS; HUMANS; INTERMEDIATE FILAMENT PROTEINS; MICE; NEOPLASTIC STEM CELLS; NERVE TISSUE PROTEINS; P-GLYCOPROTEIN; PEPTIDES; PROTO-ONCOGENE PROTEINS C-BCL-2; RECEPTOR, EPIDERMAL GROWTH FACTOR; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANSPLANTATION, HETEROLOGOUS; TUMOR MARKERS, BIOLOGICAL; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

EID: 84904817748     PISSN: 12219118     EISSN: 1842368X     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Di Tomaso, E., Pang, J. C. S., Ng, H. K., Lam, P. Y. P., Tian, X. X., Suen, K. W., Hui, A. B. Y., Hjelm, N. M. - Establishment and characterization of a human cell line from paediatric cerebellar glioblastoma multiforme. Neuropathology and Applied Neurobiology, 2000, 26:22.
2. Schiff, J. A. - An unsung hero of medical research. Retrieved on 2006-04-19. Yale Alumni Magazine, February 2002.
3. Gey, G. O., Coffman, W. D., Kubicek, M. T. - Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium, Cancer Res., 1952, 12:264.
4. Anderson, R. C., Elder, J. B., Brown, M. D., Mandingo, C. E., Parsa, A. T., Kim, P. D., Senatus, P., Anderson, D. E., Bruce, J. N. - Changes in the immunologic phenotype of human malignant glioma cells after passaging in vitro. Clinical Immunology, 2002, 102:84.
5. Perzelova, A., Macikova, I., Mraz, P., Bizik, I., Steno, J. - Characterization of two new permanent glioma cell lines 8-MG-BA and 42-MG-BA. Neoplasma, 1998, 45:25.
6. Bocchini, V., Casalone, R., Collini, P., Rebel, G., Lo Curto, F. - Changes in glial fibrillary acidic protein and karyotype during culturing of two cell lines established from human glioblastoma multiforme. Cell Tiss. Res., 1991, 265:73.
7. Osborne, M., Weber, K. - Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology. Lab. Invest., 1983, 48:372.
8. Bigner, D. D., Bigner, S. H., Ponten, J., Westermark, B., Mahaley, M. S., Ruoslahti, E., Herschman, H., Eng, L. F., Wikstrand, C. J. - Heterogeneity of genotypic and phenotypic characteristics of fifteen permanent cell lines derived from human gliomas, J. Neuropathol. Exp. Neurol., 1981, 40:201.
9. Jones, T. R., Bigner, S. H., Schold, S. C., Eng, L. F., Bigner, D. D. - Anaplastic human gliomas grown in atymic mice: morphology and glial fibrillary acidic protein expression. Am. J. Pathol., 1981, 105:316.
10. Vinores, S. A., Rubinstein, L. J. - Simultaneous expression of glial fibrillary acidic (gfa) protein and neuronspecific enolase (nse) by the same reactive or neoplastic astrocytes, Neuropathology and Applied Neurobiology, 1985, 11:349.
11. Yuan, X., Curtin, J., Xiong, Y., Liu, G., Waschsmann-Hogiu, S., Farkas, D. L., Black, K. L., Yu, J. S. - Isolation Of Cancer Stem Cells From Adult Glioblastoma Multiforme. Oncogene, 2004, 23:9392.
12. Yu, S. C., Ping, Y. F., Yi, L., Zhou, Z. H., Chen, J. H., Yao, X. H., Gao, L., Wang, J. M., Bian, X. W. - Isolation and characterization of cancer stem cells from a human glioblastoma cell line U87, Cancer Lett., 2008, 265:124.
13. Mi K Kang, Beong I Hur, Mi H Ko, Cheul H Kim, Seung H Cha, Soo K Kang - Potential identity of multipotential cancer stem-like subpopulationafter radiation of cultured brain glioma, BMC Neuroscience, 2008, 9:15 doi:10.1186/1471-2202-9-15
14. Hermansson, M., Nisttrt, M., Betsholtzt, C., Heldint, C. H., Westermarkt, B., Funa, K. - Endothelial cell hyperplasia in human glioblastoma: Coexpression of mRNA for platelet-derived growth factor (PDGF) B chain and PDGF receptor suggests autocrine growth stimulation. Proc. Nati. Acad. Sci. (USA), 1988, 85:7748. Medical Sciences
15. Hiesiger, E. M., Hayes, R., Pierz, D. M., Budzilovich, G. N. - Prognostic relevance of epidermal growth factor receptor and neu/erbB2 expression in glioblastomas. J. Neurooncol., 1993, 16:93.
16. Zhu, A., Shaeffer, J., Leslie, S., Kolm, P., El Mahdi, A. M. - Epidermal growth factor receptor: an independent predictor of survival in astrocytic tumors given definitive irradiation. Int. J. Radiat. Oncol. Biol. Phys., 1996, 34:809.
17. Korshunov, A., Golanov, A., Sycheva, R., Pronin, I. - Prognostic value of tumour associated antigen immunoreactivity and apoptosis in cerebral glioblastomas: an analysis of 168 cases. J. Clin. Pathol., 1999, 52:574.
18. Quan, A. L., Barnett, G. H., Lee, S. Y., Vogelbaum, M. A., Toms, S. A., Staugaitis, S. M. - Epidermal growth factor receptor amplification does not have prognostic significance in patients with glioblastoma multiforme. Int. J. Radiat. Oncol. Biol. Phys., 2005, 63:695.
19. Houillier, C., Lejeune, J., Benouaich-Amiel, A, Laigle-Donadey, F., Criniere, E., Mokhtari, K. - Prognostic impact of molecular markers in a series of 220 primary glioblastomas. Cancer, 2006, 106:2218.
20. Smith, J. S., Tachibana, I., Passe, S. M., Huntley, B. K., Borell, T. J., Iturria, N. - PTEN mutation, EGFR amplification, and outcome in patients with anaplastic astrocytoma and glioblastoma multiforme. J. Natl. Cancer Inst., 2001, 93:1246.
21. Krex, D., Klink, B., Hartmann, C., von Deimling, A., Pietsch, T., Simon, M., Sabel, M., Steinbach, J. P., Heese, O., Reifenberger, G., Weller, M., Schackert, G. - Long-term survival with glioblastoma multiforme. Brain, 2007, 130:2596; doi:10.1093/brain/awm204
22. Pandita, A., Aldape, K. D., Zadeh, G., Guha, A., James, C. D. - Contrasting in vivo and in vitro fates of glioblastoma cell subpopulations with amplified EGFR. Genes Chromosomes Cancer, 2004, 39:29.
23. Filmus, J., Pollak, M. N., Cairncross, J. G., Buick, R. N. - Amplified, overexpressed, and rearranged epidermal growth factor receptor gene in a human astrocytoma cell line. Biochem. Biophys. Res. Commun., 1985, 131:207.
24. Thomas, C., Ely, G., James, C. D., Jenkins, R., Kastan, M., Jedlicka, A., Burger, P., Wharen, R. - Glioblastomarelated gene mutations and over-expression of functional epidermal growth factor receptors in SKMG-3 glioma cells. Acta Neuropathol., 2001, 101:605.
25. Horten, B. C., Basler, G. A., and Shapiro, W. R. - Xenograft of human malignant glial tumors into brains of nude mice. A histopathological study. J. Neuropathol. Exp. Neurol., 1981, 40:493.
26. Shapiro, W. R., Basler, G. A., Chernik, N. L., Posner, J. B., Human brain tumor transplantation into nude mice. J. Natl. Cancer Inst., 1979, 62:447.
27. Giannini, C., Sarkaria, J. N., Saito, A., Uhm, J. H., Galanis, E., Carlson, B. L., Schroeder, M. A., James, C. D. - Patient tumor EGFR and PDGFRA gene amplifi cations retained in an invasive intracranial xenograft model of glioblastoma multiforme. Neuro-Oncology, 2005, 7:164.