A droplet microfluidic approach to single-stream nucleic acid isolation and mutation detection

Microfluidics and Nanofluidics

Volumn 17, Issue 2, 2014, Pages 425-430

  Shin, Dong Jin ^a   Zhang, Yi ^a   Wang, Tza Huei ^a,b,c  

^a Johns Hopkins University   (United States)

^b Johns Hopkins University   (United States)

^c JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

Droplet microfluidics; Droplet PCR; Melting curve analysis; Mutation detection

Indexed keywords

BIOINFORMATICS; DROPS; MELTING; POLYMERASE CHAIN REACTION;

CODON 12; DNA MELTING; DROPLET MICROFLUIDICS; GENETIC MUTATIONS; MELTING CURVE ANALYSIS; MUTATION DETECTION; WILD TYPES;

MICROFLUIDICS;

EID: 84904789133     PISSN: 16134982     EISSN: 16134990     Source Type: Journal    
DOI: 10.1007/s10404-013-1305-7     Document Type: Article

References (23)

1. Chiou CH, Shin DJ, Zhang Y, Wang TH (2013) Topography-assisted electromagnetic platform for blood-to-PCR in a droplet. Biosens Bioelectron 50:91-99
2. Easley CJ, Karlinsey JM, Bienvenue JM, Legendre LA, Roper MG, Feldman SH, Hughes MA, Hewlett EL, Merkel TJ, Ferrance JP, Landers JP (2006) A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability. Proc Natl Acad Sci USA 103(51):19272-19277
3. Griffiths-Chu S, Patterson JA, Berger CL, Edelson RL, Chu AC (1984) Characterization of immature T cell subpopulations in neonatal blood. Blood 64(1):296-300
4. Ho CL, Kurman RJ, Dehari R, Wang T, Shih IM (2004) Mutations of BRAF and KRAS precede the development of ovarian serous borderline tumors. Cancer Res 64(19):6915-6918
5. Lehmann U, Hadjidj S, Parashar VK, Vandevyver C, Rida A, Gijs MAM (2006) Two-dimensional magnetic manipulation of microdroplets on a chip as a platform for bioanalytical applications. Sens Actuators B Chem 117(2):457-463
6. Mariella R Jr (2008) Sample preparation: the weak link in microfluidics-based biodetection. Biomed Microdevices 10(6):777-784
7. Melzak KA, Sherwood CS, Turner RFB, Haynes CA (1996) Driving forces for DNA adsorption to silica in perchlorate solutions. J Colloid Interface Sci 181:635-644
8. Miller EM, Wheeler AR (2008) A digital microfluidic approach to homogeneous enzyme assays. Anal Chem 80(5):1614-1619
9. Ohlander A, Zillo C, Hammerle T, Zelenin S, Klink G, Chiari M, Bock K, Russom A (2013) Genotyping of single nucleotide polymorphisms by melting curve analysis using thin film semi-transparent heaters integrated in a lab-on-foil system. Lab Chip 13:2075-2082
10. Pal R, Yang M, Lin R, Johnson BN, Srivastava N, Razzacki SZ, Chomistek KJ, Heldsinger DC, Haque RM, Ugaz VM, Thwar PK, Chen Z, Alfano K, Yim MB, Krishnan M, Fuller AO, Larson RG, Burke DT, Burns MA (2005) An integrated microfluidic device for influenza and other genetic analyses. Lab Chip 5(10):1024-1032
11. Palais R, Wittwer CT (2009) Chapter 13 mathematical algorithms for high-resolution DNA melting analysis. In: Johnson ML, Brand L (eds) Methods in enzymology, vol 454. Academic Press, New York, pp 323-343
12. Park S, Zhang Y, Lin S, Wang TH, Yang S (2011) Advances in microfluidic PCR for point-of-care infectious disease diagnostics. Biotechnol Adv 29(6):830-839
13. Pipper J, Inoue M, Ng LFP, Neuzil P, Zhang Y, Novak L (2007) Catching bird flu in a droplet. Nat Med 13(10):1259-1263
14. Saiki RK, Chang CA, Levenson CH, Warren TC, Boehm CD, Kazazian HH, Erlich HA (1988) Diagnosis of sickle cell anemia and beta-thalassemia with enzymatically amplified DNA and nonradioactive allele-specific oligonucleotide probes. N Engl J Med 319:537-541
15. Srinivasan V, Pamula VK, Fair RB (2004) An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human physiological fluids. Lab Chip 4(4):310-315
16. Tsiatis AC, Norris-Kirby A, Rich RG, Hafez MJ, Gocke CD, Eshleman JR, Murphy KM (2010) Comparison of Sanger sequencing, pyrosequencing, and melting curve analysis for the detection of KRAS mutations: diagnostic and clinical implications. J Mol Diagn 12(4):425-432
17. Velev OD, Prevo BG, Bhatt KH (2003) On-chip manipulation of free droplets. Nature 426(6966):515-516
18. Wittwer CT (2009) High-resolution DNA melting analysis: advancements and limitations. Hum Mutat 30(6):857-859
19. Wittwer CT, Reed GH, Gundry CN, Vanderstenn JG, Pryor RJ (2003) High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem 49(6):853-860
20. Wu DY, Ugozzoli L, Pal BK, Wallace RB (1989) Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia. Proc Natl Acad Sci USA 86(8):2757-2760
21. Zhang Y, Wang TH (2013) Full-range magnetic manipulation of droplets via surface energy traps enables complex bioassays. Adv Mater 25(21):2903-2908
22. Zhang Y, Park S, Yang S, Wang TH (2010) An all-in-one microfluidic device for parallel DNA extraction and gene analysis. Biomed Microdevices 12(6):1043-1049
23. Zhang Y, Park S, Liu K, Tsuan J, Yang S, Wang TH (2011) A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification. Lab Chip 11(3):398-406