Inhibition of p38 mitogen-activated protein kinase attenuates butyrate-induced intestinal barrier impairment in a Caco-2 cell monolayer model

Journal of Pediatric Gastroenterology and Nutrition

Volumn 59, Issue 2, 2014, Pages 264-269

  Huang, Xiao Zhong ^a   Li, Zhong Rong ^a   Zhu, Li Bin ^a   Huang, Hui Ya ^a   Hou, Long Long ^a   Lin, Jing ^b  

^a Medical University   (China)

^b MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

barrier function; butyrate; intestinal mucosa; p38 MAPK; short chain fatty acid

Indexed keywords

4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL)IMIDAZOLE; BETA TUBULIN; BUTYRIC ACID; MITOGEN ACTIVATED PROTEIN KINASE P38; BUTYRIC ACID DERIVATIVE; ENZYME INHIBITOR; FLUORESCEIN ISOTHIOCYANATE; IMIDAZOLE DERIVATIVE; INULIN; LIPOCORTIN 5; PYRIDINE DERIVATIVE;

APOPTOSIS; ARTICLE; CELL DEATH; CONTROLLED STUDY; CYTOTOXICITY; ELECTRIC RESISTANCE; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME PHOSPHORYLATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; INTESTINAL BARRIER IMPAIRMENT; INTESTINE EPITHELIUM CELL; INTESTINE FUNCTION DISORDER; INTESTINE MUCOSA PERMEABILITY; PRIORITY JOURNAL; PROTEIN EXPRESSION; SCANNING ELECTRON MICROSCOPY; SIGNAL TRANSDUCTION; TRANSEPITHELIAL ELECTRICAL RESISTANCE; ANTAGONISTS AND INHIBITORS; CACO 2 CELL LINE; DRUG EFFECTS; ENTEROPATHY; ENZYMOLOGY; IMPEDANCE; INTESTINE ABSORPTION; INTESTINE MUCOSA; METABOLISM; PERMEABILITY; PHOSPHORYLATION;

ANNEXIN A5; APOPTOSIS; BUTYRATES; CACO-2 CELLS; ELECTRIC IMPEDANCE; ENZYME INHIBITORS; FLUORESCEIN-5-ISOTHIOCYANATE; HUMANS; IMIDAZOLES; INTESTINAL ABSORPTION; INTESTINAL DISEASES; INTESTINAL MUCOSA; INULIN; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PERMEABILITY; PHOSPHORYLATION; PYRIDINES;

EID: 84904767192     PISSN: 02772116     EISSN: 15364801     Source Type: Journal    
DOI: 10.1097/MPG.0000000000000369     Document Type: Article

References (29)

1. Walker WA. Development of the intestinal mucosal barrier. J Pediatr Gastroenterol Nutr 2002;34:S33-9.
2. de Santa Barbara P, van den Brink GR, Roberts DJ. Development and differentiation of the intestinal epithelium. Cell Mol Life Sci 2003; 60:1322-32.
3. Neu J, Walker WA. Necrotizing enterocolitis. N Engl J Med 2011; 364:255-64.
4. Gibbs K, Lin J, Holzman IR. Necrotizing enterocolitis: the state of the science. Indian J Pediatr 2007;74:67-72.
5. Roy CC, Kien CL, Bouthillier L, et al. Short-chain fatty acids: ready for prime time? Nutr Clin Pract 2006;4:351-66.
6. Bond JH, Levitt MD. Fate of soluble carbohydrate in the colon of rats and man. J Clin Invest 1976;57:1158-64.
7. Sangild PT, Siggers RH, Schmidt M, et al. Diet- and colonizationdependent intestinal dysfunction predisposes to necrotizing enterocolitis in preterm pigs. Gastroenterology 2006;130:1776-92.
8. Lin J. Too much short chain fatty acids cause neonatal necrotizing enterocolitis. Med Hypotheses 2004;62:291-3.
9. Peng L, He Z, Chen W, et al. Effects of butyrate on intestinal barrier function in a Caco-2 cell monolayer model of intestinal barrier. Pediatr Res 2007;61:37-41.
10. Han J, Lee JD, Bibbs L, et al. A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells. Science 1994;265:808-11.
11. Sumbayev VV, Yasinska IM. Role of MAP kinase-dependent apoptotic pathway in innate immune responses and viral infection. Scand J Immunol 2006;63:391-400.
12. Cuadrado A, Nebreda AR. Mechanisms and functions of p38 MAPK signalling. Biochem J 2010;429:403-17.
13. Cho SD, Ahn NS, Jung JW, et al. Critical role of the c-JunNH2-terminal kinase and p38 mitogen-activated protein kinase pathways on sodium butyrate-induced apoptosis in DU145 human prostate cancer cells. Eur J Cancer Prev 2006;15:57-63.
14. Peng L, Li ZR, Green RS, et al. Butyrate enhances the intestinal barrier by facilitating tight junction assembly via activation of AMP activated protein kinase in Caco-2 cell monolayers. J Nutr 2009;139:1619-25.
15. Lin J. Effects of short chain fatty acids on the intestinal barrier. Curr Nutr Food Sci 2013;9:93-8.
16. Lin J, Nafday SM, Chauvin SN, et al. Variable effects of short chain fatty acids and lactic acid in inducing intestinal mucosal injury in newborn rats. J Pediatr Gastroenterol Nutr 2002;35:545-50.
17. Nafday SM, Chen W, Peng L, et al. Short-chain fatty acids induce colonic mucosal injury in rats with various postnatal ages. Pediatr Res 2005;57:201-4.
18. Sakurazawa T, Ohkusa T. Cytotoxicity of organic acids produced by anaerobic intestinal bacteria on cultured epithelial cells. J Gastroenterol 2005;40:600-9.
19. Vachon PH, Cardin E, Harnois C, et al. Early establishment of epithelial apoptosis in the developing human small intestine. Int J Dev Biol 2000;44:891-8.
20. Bojarski C, Gitter AH, Bendfeldt K, et al. Permeability of human HT-29/B6 colonic epithelium as a function of apoptosis. J Physiol 2001; 535:541-52.
21. Ruemmele FM, Schwartz S, Seidman EG, et al. Butyrate induced Caco- 2 cell apoptosis is mediated via the mitochondrial pathway. Gut 2003;52:94-100.
22. Shao Y, Gao Z, Marks PA, et al. Apoptotic and autophagic cell death induced by histone deacetylase inhibitors. Proc Natl Acad Sci U S A 2004;101:18030-5.
23. Li CJ, Elsasser TH. Butyrate-induced apoptosis and cell cycle arrest in bovine kidney epithelial cells: involvement of caspase and proteasome pathways. J Anim Sci 2005;83:89-97.
24. Schwab M, Reynders V, Ulrich S, et al. PPARgamma is a key target of butyrate-induced caspase-3 activation in the colorectal cancer cell line Caco-2. Apoptosis 2006;11:1801-11.
25. Cuenda A, Rousseau S. p38 MAP-kinases pathway regulation, function and role in human diseases. Biochim Biophys Acta 2007;1773:1358-75.
26. McMillan L, Butcher SK, Pongracz J, et al. Opposing effects of butyrate and bile acids on apoptosis of human colon adenoma cells: differential activation of PKC and MAP kinases. Br J Cancer 2003;88:748-53.
27. Fung KY, Brierley GV, Henderson S, et al. Butyrate-induced apoptosis in HCT116 colorectal cancer cells includes induction of a cell stress response. J Proteome Res 2011;10:1860-9.
28. Cummings JH, Pomare EW, Branch WJ, et al. Short chain fatty acids in human large intestine, portal, hepatic and venous blood. Gut 1987; 28:1221-7.
29. Ogawa K, Ben RA, Pons S, et al. Volatile fatty acids, lactic acid and pH in stools of breast-fed and bottle-fed infants. J Pediatr Gastroenterol Nutr 1992;15:248-52.