[ureido-15N]Citrulline UPLC-MS/MS nitric oxide synthase (NOS) activity assay: Development, validation, and applications to assess NOS uncoupling and human platelets NOS activity

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 965, Issue , 2014, Pages 173-182

  Böhmer, Anke ^a   Gambaryan, Stepan ^b,c   Flentje, Markus ^a   Jordan, Jens ^a   Tsikas, Dimitrios ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

^c SECHENOV INSTITUTE OF EVOLUTIONARY PHYSIOLOGY AND BIOCHEMISTRY   (Russian Federation)

Author keywords

L Citrulline; Nitric oxide (NO); NO synthase; Quantification; Tandem mass spectrometry; Validation

Indexed keywords

ASSAYS; ISOTOPES; MASS SPECTROMETRY; METABOLISM; NITRATES; NITRIC OXIDE; OXYGEN; PLATELETS; POSITIVE IONS; SUBSTRATES; UREA;

L-CITRULLINE; QUANTIFICATION; SYNTHASES; TANDEM MASS SPECTROMETRY; VALIDATION;

AMINO ACIDS;

AMMONIA; CITRULLINE; DEUTERIUM; GLUTATHIONE DISULFIDE; INDUCIBLE NITRIC OXIDE SYNTHASE; NITRATE; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; NITRITE; NITROGEN 15; POTASSIUM; RADIOISOTOPE; SODIUM; TROMETAMOL; UNCLASSIFIED DRUG; [GUANIDINO NITROGEN 15] L ARGININE; NITROGEN;

ARTICLE; CATION EXCHANGE; CELL ISOLATION; COLLISIONALLY ACTIVATED DISSOCIATION; CONTROLLED STUDY; ENZYME ACTIVITY; FEMALE; HUMAN; HUMAN CELL; HYDROPHILIC INTERACTION CHROMATOGRAPHY; IN VITRO STUDY; ISOTOPE LABELING; LIMIT OF DETECTION; LIQUID CHROMATOGRAPHY; MASS FRAGMENTOGRAPHY; MYCOBACTERIUM SMEGMATIS; NORMAL HUMAN; OXIDATIVE PHOSPHORYLATION UNCOUPLING; PH; PRIORITY JOURNAL; QUALITY CONTROL; QUANTITATIVE ANALYSIS; SOLID PHASE EXTRACTION; TANDEM MASS SPECTROMETRY; THROMBOCYTE; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; VALIDATION STUDY; ANALOGS AND DERIVATIVES; BLOOD; CHEMISTRY; ENZYMOLOGY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; PROCEDURES; REPRODUCIBILITY;

BLOOD PLATELETS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CITRULLINE; HUMANS; LIMIT OF DETECTION; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; NITROGEN ISOTOPES; REPRODUCIBILITY OF RESULTS; TANDEM MASS SPECTROMETRY;

EID: 84904296542     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2014.06.025     Document Type: Article

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