Acute response and subcellular movement of HSP27, αB-crystallin and HSP70 in human skeletal muscle after blood-flow-restricted low-load resistance exercise

Acta Physiologica

Volumn 211, Issue 4, 2014, Pages 634-646

  Cumming, K T ^a   Paulsen, G ^a   Wernbom, M ^a,b   Ugelstad, I ^a   Raastad, T ^a  

^a NORWEGIAN SCHOOL OF SPORT SCIENCES   (Norway)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

Glycogen staining; Muscle damage; Occlusion training; Stress proteins

Indexed keywords

ALPHA CRYSTALLIN; DESMIN; GLYCOGEN; HEAT SHOCK PROTEIN 27; HEAT SHOCK PROTEIN 70; MYOSIN HEAVY CHAIN; CRYAB PROTEIN, HUMAN; HSPB1 PROTEIN, HUMAN;

ARTICLE; BLOOD FLOW RESTRICTED LOW LOAD RESISTANCE EXERCISE; CYTOSOL; CYTOSOLIC FRACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; GLYCOGEN ANALYSIS; HUMAN; HUMAN EXPERIMENT; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MUSCLE BIOPSY; MUSCLE BLOOD FLOW; MUSCLE ISOMETRIC CONTRACTION; MUSCLE STRENGTH; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; RESISTANCE TRAINING; SKELETAL MUSCLE; STRESS; WESTERN BLOTTING; ADULT; FEMALE; MALE; METABOLISM; PHYSIOLOGY; PROTEIN TRANSPORT; YOUNG ADULT;

ADULT; ALPHA-CRYSTALLIN B CHAIN; BLOTTING, WESTERN; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HSP27 HEAT-SHOCK PROTEINS; HSP70 HEAT-SHOCK PROTEINS; HUMANS; IMMUNOHISTOCHEMISTRY; MALE; MUSCLE, SKELETAL; PROTEIN TRANSPORT; RESISTANCE TRAINING; YOUNG ADULT;

EID: 84904262229     PISSN: 17481708     EISSN: 17481716     Source Type: Journal    
DOI: 10.1111/apha.12305     Document Type: Article

References (46)

1. Armstrong, S.C., Delacey, M. & Ganote, C.E. 1999. Phosphorylation state of hsp27 and p38 MAPK during preconditioning and protein phosphatase inhibitor protection of rabbit cardiomyocytes. J Mol Cell Cardiol 31, 555-567.
2. Asp, S., Daugaard, J.R. & Richter, E.A. 1995. Eccentric exercise decreases glucose transporter GLUT4 protein in human skeletal muscle. J Physiol 482, 705-712.
3. Asp, S., Daugaard, J.R., Kristiansen, S., Kiens, B. & Richter, E.A. 1996. Eccentric exercise decreases maximal insulin action in humans: muscle and systemic effects. J Physiol 494, 891-898.
4. Barash, I.A., Peters, D., Fridén, J., Lutz, G.J. & Lieber, R.L. 2002. Desmin cytoskeletal modifications after a bout of eccentric exercise in the rat. Am J Physiol Regul Integr Comp Physiol 283, R958-R963.
5. Baumeister, S., Ofer, N., Kleist, C., Terne, P., Opelz, G., Gebhard, M.M., Germann, G. & Heitmann, C. 2004. Reduction of skeletal muscle injury in composite tissue allotransplantation by heat stress preconditioning. Plast Reconstr Surg 114, 1832-1841.
6. Febbraio, M.A., Steensberg, A., Walsh, R., Koukoulas, I., van Hall, G., Saltin, B. & Pedersen, B.K. 2002. Reduced glycogen availability is associated with an elevation in HSP72 in contracting human skeletal muscle. J Physiol 538, 911-917.
7. Folkesson, M., Mackey, A.L., Holm, L., Kjaer, M., Paulsen, G., Raastad, T., Henriksson, J. & Kadi, F. 2008. Immunohistochemical changes in the expression of HSP27 in exercised human vastus lateralis muscle. Acta Physiol 194, 215-222.
8. Folkesson, M., Mackey, A.L., Langberg, H., Oskarsson, E., Piehl-Aulin, K., Henriksson, J. & Kadi, F. 2013. The expression of heat shock protein in human skeletal muscle: effects of muscle fibre phenotype and training background. Acta Physiol 4, 1-8.
9. Fridén, J., Sjöström, M. & Ekblom, B. 1983. Myofibrillar damage following intense eccentric exercise in man. Int J Sports Med 4, 170-176.
10. Gjøvaag, T.F. & Dahl, H.A. 2006. Effect of training and detraining on the expression of heat shock proteins in m. triceps brachii of untrained males and females. Eur J Appl Physiol 98, 310-322.
11. Golenhofen, N., Perng, M.Der., Quinlan, R.A. & Drenckhahn, D. 2004. Comparison of the small heat shock proteins alphaB-crystallin, MKBP, HSP25, HSP20, and cvHSP in heart and skeletal muscle. Histochem Cell Biol 122, 415-425.
12. Iversen, E. & Røstad, V. 2010. Low-load ischemic exercise-induced rhabdomyolysis. Clin J Sport Med 20, 218-219.
13. Jackson, M.J., Khassaf, M., Vasilaki, A., McArdle, F. & McArdle, A. 2004. Vitamin E and the oxidative stress of exercise. Ann N Y Acad Sci 1031, 158-168.
14. Jørgensen, A., Foster, P.P., Eftedal, I., Wisløff, U., Paulsen, G., Havnes, M.B. & Brubakk, A.O. 2013. Exercise-induced myofibrillar disruption with sarcolemmal integrity prior to simulated diving has no effect on vascular bubble formation in rats. Eur J Appl Physiol 113, 1189-1198.
15. Kacin, A. & Strazar, K. 2011. Frequent low-load ischemic resistance exercise to failure enhances muscle oxygen delivery and endurance capacity. Scand J Med Sci Sports 21, e231-e241.
16. Khassaf, M., Child, R.B., McArdle, A., Brodie, D.A., Esanu, C. & Jackson, M.J. 2001. Time course of responses of human skeletal muscle to oxidative stress induced by nondamaging exercise. J Appl Physiol 90, 1031-1035.
17. Khassaf, M., McArdle, A., Esanu, C., Vasilaki, A., McArdle, F., Griffiths, R.D., Brodie, D.A. & Jackson, M.J. 2003. Effect of vitamin C supplements on antioxidant defence and stress proteins in human lymphocytes and skeletal muscle. J Physiol 549, 645-652.
18. Koh, T.J. 2002. Do small heat shock proteins protect skeletal muscle from injury? Exerc Sport Sci Rev 30, 117-121.
19. Koh, T.J. & Escobedo, J. 2004. Cytoskeletal disruption and small heat shock protein translocation immediately after lengthening contractions. Am J Physiol Cell Physiol 286, C713-C722.
20. Krustrup, P., Söderlund, K., Relu, M.U., Ferguson, R.A. & Bangsbo, J. 2009. Heterogeneous recruitment of quadriceps muscle portions and fibre types during moderate intensity knee-extensor exercise: effect of thigh occlusion. Scand J Med Sci Sports 19, 576-584.
21. Larkins, N.T., Murphy, R.M. & Lamb, G.D. 2012. Absolute amounts and diffusibility of HSP72, HSP25, and αB-crystallin in fast- and slow-twitch skeletal muscle fibers of rat. Am J Physiol Cell Physiol 302, C228-C239.
22. Lepore, D.A., Hurley, J.V., Stewart, A.G., Morrison, W.A. & Anderson, R.L. 2000. Prior heat stress improves survival of ischemic-reperfused skeletal muscle in vivo. Muscle Nerve 23, 1847-1855.
23. Maglara, A., Vasilaki, A., Jackson, M. & McArdle, A. 2003. Damage to developing mouse skeletal muscle myotubes in culture: protective effect of heat shock proteins. J Physiol 548, 837-846.
24. McArdle, A., Dillmann, W.H., Mestril, R., Faulkner, J.A. & Jackson, M.J. 2004. Overexpression of HSP70 in mouse skeletal muscle protects against muscle damage and age-related muscle dysfunction. FASEB J 18, 355-357.
25. Moritani, T., Sherman, W.M., Shibata, M., Matsumoto, T. & Shinohara, M. 1992. Oxygen availability and motor unit activity in humans. Eur J Appl Physiol Occup Physiol 64, 552-556.
26. Morton, J.P., MacLaren, D.P.M., Cable, N.T., Bongers, T., Griffiths, R.D., Campbell, I.T., Evans, L., Kayani, A., McArdle, A. & Drust, B. 2006. Time course and differential responses of the major heat shock protein families in human skeletal muscle following acute nondamaging treadmill exercise. J Appl Physiol 101, 176-182.
27. Morton, J.P., Holloway, K., Woods, P., Cable, N.T., Burniston, J., Evans, L., Kayani, A.C. & McArdle, A. 2009a. Exercise training-induced gender-specific heat shock protein adaptations in human skeletal muscle. Muscle Nerve 39, 230-233.
28. Morton, J.P., Kayani, A.C., McArdle, A. & Drust, B. 2009b. The exercise-induced stress response of skeletal muscle, with specific emphasis on humans. Sports Med 39, 643-662.
29. Mymrikov, E.V., Seit-Nebi, A.S. & Gusev, N.B. 2011. Large potentials of small heat shock proteins. Physiol Rev 91, 1123-1159.
30. Noble, E.G., Milne, K.J. & Melling, C.W.J. 2008. Heat shock proteins and exercise: a primer. Appl Physiol Nutr Metab 33, 1050-1065.
31. Paulsen, G., Vissing, K., Kalhovde, J.M.J.M., Ugelstad, I., Bayer, M.L.M.L., Kadi, F., Schjerling, P., Hallén, J. & Raastad, T. 2007. Maximal eccentric exercise induces a rapid accumulation of small heat shock proteins on myofibrils and a delayed HSP70 response in humans. Am J Physiol Regul Integr Comp Physiol 293, R844-R853.
32. Paulsen, G., Lauritzen, F., Bayer, M.L., Kalhovde, J.M., Ugelstad, I., Owe, S.G., Hallén, J., Bergersen, L.H. & Raastad, T. 2009. Subcellular movement and expression of HSP27, alphaB-crystallin, and HSP70 after two bouts of eccentric exercise in humans. J Appl Physiol 107, 570-582.
33. Paulsen, G., Mikkelsen, U.R., Raastad, T. & Peake, J.M. 2012. Leucocytes, cytokines and satellite cells: what role do they play in muscle damage and regeneration following eccentric exercise? Exerc Immunol Rev 18, 42-97.
34. Ruifrok, A.C. & Johnston, D.A. 2001. Quantification of histochemical staining by color deconvolution. Anal Quant Cytol Histol 23, 291-299.
35. Suga, T., Okita, K., Morita, N., Yokota, T., Hirabayashi, K., Horiuchi, M., Takada, S., Takahashi, T., Omokawa, M., Kinugawa, S. & Tsutsui, H. 2009. Intramuscular metabolism during low-intensity resistance exercise with blood flow restriction. J Appl Physiol 106, 1119-1124.
36. Takarada, Y., Nakamura, Y., Aruga, S., Onda, T., Miyazaki, S. & Ishii, N. 2000. Rapid increase in plasma growth hormone after low-intensity resistance exercise with vascular occlusion. J Appl Physiol 88, 61-65.
37. Thompson, H.S., Clarkson, P.M. & Scordilis, S.P. 2002. The repeated bout effect and heat shock proteins: intramuscular HSP27 and HSP70 expression following two bouts of eccentric exercise in humans. Acta Physiol Scand 174, 47-56.
38. Thompson, H.S., Maynard, E.B., Morales, E.R. & Scordilis, S.P. 2003. Exercise-induced HSP27, HSP70 and MAPK responses in human skeletal muscle. Acta Physiol Scand 178, 61-72.
39. Touchberry, C.D., Gupte, A.A., Bomhoff, G.L., Graham, Z.A., Geiger, P.C. & Gallagher, P.M. 2012. Acute heat stress prior to downhill running may enhance skeletal muscle remodeling. Cell Stress Chaperones 17, 693-705.
40. Tupling, A.R., Bombardier, E., Stewart, R.D., Vigna, C. & Aqui, A.E. 2007. Muscle fiber type-specific response of Hsp70 expression in human quadriceps following acute isometric exercise. J Appl Physiol 103, 2105-2111.
41. Umbel, J.D., Hoffman, R.L., Dearth, D.J., Chleboun, G.S., Manini, T.M. & Clark, B.C. 2009. Delayed-onset muscle soreness induced by low-load blood flow-restricted exercise. Eur J Appl Physiol 107, 687-695.
42. Wernbom, M., Augustsson, J. & Raastad, T. 2008. Ischemic strength training: a low-load alternative to heavy resistance exercise? Scand J Med Sci Sports 18, 401-416.
43. Wernbom, M., Järrebring, R., Andreasson, M.A. & Augustsson, J. 2009. Acute effects of blood flow restriction on muscle activity and endurance during fatiguing dynamic knee extensions at low load. J Strength Cond Res 23, 2389-2395.
44. Wernbom, M., Paulsen, G., Nilsen, T.S., Hisdal, J. & Raastad, T. 2012. Contractile function and sarcolemmal permeability after acute low-load resistance exercise with blood flow restriction. Eur J Appl Physiol 112, 2051-2063.
45. Wernbom, M., Apro, W., Paulsen, G., Nilsen, T.S., Blomstrand, E. & Raastad, T. 2013. Acute low-load resistance exercise with and without blood flow restriction increased protein signalling and number of satellite cells in human skeletal muscle. Eur J Appl Physiol 113, 2953-2965.
46. Yoshida, K., Aki, T., Harada, K., Shama, K.M.A., Kamoda, Y., Suzuki, A. & Ohno, S. 1999. Translocation of HSP27 and MKBP in Ischemic Heart. Cell Struct Funct 24, 181-185.