Comparative dose-responses of recombinant human IL-2 and IL-7 on STAT5 phosphorylation in CD4+FOXP3- cells versus regulatory T cells: A whole blood perspective

Cytokine

Volumn 69, Issue 1, 2014, Pages 146-149

  Dupont, Guillaume ^a,b   Demaret, Julie ^a,c   Venet, Fabienne ^a,c   Malergue, Fabrice ^d   Malcus, Christophe ^a   Poitevin Later, Françoise ^a   Morel, JérÔme ^b   Monneret, Guillaume ^a,c  

^a EDOUARD HERRIOT HOSPITAL   (France)

^b UNIVERSITY HOSPITAL OF SAINT ETIENNE   (France)

^c UNIVERSITÉ LYON 1   (France)

^d Immunotech   (France)

Author keywords

Flow cytometry; FOXP3; Interleukin 2; Interleukin 7; STAT5

Indexed keywords

RECOMBINANT INTERLEUKIN 2; RECOMBINANT INTERLEUKIN 7; STAT5 PROTEIN; TRANSCRIPTION FACTOR FOXP3; FORKHEAD TRANSCRIPTION FACTOR; FOXP3 PROTEIN, HUMAN; IL2 PROTEIN, HUMAN; IL7 PROTEIN, HUMAN; INTERLEUKIN 2; INTERLEUKIN 7; RECOMBINANT PROTEIN; STAT5A PROTEIN, HUMAN; TUMOR SUPPRESSOR PROTEIN;

ADULT; ANALYTIC METHOD; ARTICLE; BLOOD; CD4+ T LYMPHOCYTE; CELL STIMULATION; CLINICAL PRACTICE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG MECHANISM; DRUG MONITORING; EX VIVO STUDY; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; INTRACELLULAR SIGNALING; MALE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; REGULATORY T LYMPHOCYTE; T LYMPHOCYTE ACTIVATION; WHOLE BLOOD SINGLE TUBE ASSAY; DOSE RESPONSE; DRUG EFFECTS; IMMUNOLOGY; LYMPHOCYTE ACTIVATION; METABOLISM; MIDDLE AGED; PHOSPHORYLATION;

ERAGROSTIS TEF;

DOSE-RESPONSE RELATIONSHIP, DRUG; FEMALE; FLOW CYTOMETRY; FORKHEAD TRANSCRIPTION FACTORS; HUMANS; INTERLEUKIN-2; INTERLEUKIN-7; LYMPHOCYTE ACTIVATION; MALE; MIDDLE AGED; PHOSPHORYLATION; RECOMBINANT PROTEINS; STAT5 TRANSCRIPTION FACTOR; T-LYMPHOCYTES, REGULATORY; TUMOR SUPPRESSOR PROTEINS;

EID: 84904159323     PISSN: 10434666     EISSN: 10960023     Source Type: Journal    
DOI: 10.1016/j.cyto.2014.05.021     Document Type: Article

References (10)

1. Rochman Y., Spolski R., Leonard W.J. New insights into the regulation of T cells by gamma(c) family cytokines. Nat Rev Immunol 2009, 9:480-490.
2. Atkins M.B. Interleukin-2: clinical applications. Semin Oncol 2002, 29:12-17.
3. Mackall C.L., Fry T.J., Gress R.E. Harnessing the biology of IL-7 for therapeutic application. Nat Rev Immunol 2011, 11:330-342.
4. Saadoun D., Rosenzwajg M., Joly F., Six A., Carrat F., Thibault V., et al. Regulatory T-cell responses to low-dose interleukin-2 in HCV-induced vasculitis. N Engl J Med 2011, 365:2067-2077.
5. Koreth J., Matsuoka K., Kim H.T., McDonough S.M., Bindra B., Alyea E.P., et al. Interleukin-2 and regulatory T cells in graft-versus-host disease. N Engl J Med 2011, 365:2055-2066.
6. Wuest T.Y., Willette-Brown J., Durum S.K., Hurwitz A.A. The influence of IL-2 family cytokines on activation and function of naturally occurring regulatory T cells. J Leukoc Biol 2008, 84:973-980.
7. Simonetta F., Chiali A., Cordier C., Urrutia A., Girault I., Bloquet S., et al. Increased CD127 expression on activated FOXP3+CD4+ regulatory T cells. Eur J Immunol 2010, 40:2528-2538.
8. Vang K.B., Yang J., Mahmud S.A., Burchill M.A., Vegoe A.L., Farrar M.A. IL-2, -7, and -15, but not thymic stromal lymphopoeitin, redundantly govern CD4+Foxp3+ regulatory T cell development. J Immunol 2008, 181:3285-3290.
9. Maecker H.T., McCoy J.P., Nussenblatt R. Standardizing immunophenotyping for the Human Immunology Project. Nat Rev Immunol 2012, 12:191-200.
10. Perez O.D., Mitchell D., Campos R., Gao G.J., Li L., Nolan G.P. Multiparameter analysis of intracellular phosphoepitopes in immunophenotyped cell populations by flow cytometry. Curr Protoc Cytom 2005, Chapter 6, Unit 6 20.