Metabolic de-isotoping for improved LC-MS characterization of modified RNAs

Journal of the American Society for Mass Spectrometry

Volumn 25, Issue 7, 2014, Pages 1114-1123

  Wetzel, Collin ^a   Li, Siwei ^a   Limbach, Patrick A ^a  

^a UNIVERSITY OF CINCINNATI   (United States)

Author keywords

Comparative analysis; Liquid chromatography mass spectrometry; ncRNA; Post transcriptional modifications; Quantitative analysis; RNA sequencing; RNase mass mapping; tRNA

Indexed keywords

CHEMICAL ANALYSIS; DATA PROCESSING; ISOTOPES; MAPPING; MASS SPECTROMETRY;

COMPARATIVE ANALYSIS; LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY; MASS MAPPING; NCRNA; POST-TRANSCRIPTIONAL MODIFICATION; RNA SEQUENCING; TRNA;

RNA;

CARBON; CARBON 13; ISOTOPE; NITROGEN 15; OXYGEN; OXYGEN 18; RIBONUCLEASE; RIBONUCLEASE T1; TRANSFER RNA; UNTRANSLATED RNA; BACTERIAL RNA;

ANALYTIC METHOD; ARTICLE; COMPARATIVE ANALYSIS OF RNA DIGEST; DATA PROCESSING; GENE DELETION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; RADIOISOTOPE DISTRIBUTION; SEQUENCE HOMOLOGY; STANDARD; CHEMISTRY; ESCHERICHIA COLI; METABOLISM; MOLECULAR WEIGHT; PROCEDURES; RNA PROCESSING; SEQUENCE ANALYSIS; TANDEM MASS SPECTROMETRY;

CARBON ISOTOPES; CHROMATOGRAPHY, LIQUID; ESCHERICHIA COLI; MOLECULAR WEIGHT; RIBONUCLEASES; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, BACTERIAL; RNA, TRANSFER; RNA, UNTRANSLATED; SEQUENCE ANALYSIS, RNA; TANDEM MASS SPECTROMETRY;

EID: 84904039539     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-014-0889-9     Document Type: Article

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