Minimizing 18O/16O back-exchange in the relative quantification of ribonucleic acids

Journal of Mass Spectrometry

Volumn 44, Issue 8, 2009, Pages 1195-1202

  Castleberry, Colette M ^a   Lilleness, Kathleen ^a,b   Baldauff, Rachel ^a,c   Limbach, Patrick A ^a  

^a UNIVERSITY OF CINCINNATI   (United States)

^b WHITWORTH UNIVERSITY   (United States)

^c ROSE HULMAN INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

MALDI MS; Quantitative analysis; Ribonucleic acids; Transfer RNA

Indexed keywords

ACCURACY AND PRECISION; DIGESTION PRODUCTS; ENDONUCLEASES; EXCHANGE PROCESS; INCUBATION TEMPERATURES; ISOTOPICALLY LABELED; MALDI-MS; MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY; PH VALUE; QUANTITATIVE ANALYSIS; RELATIVE QUANTIFICATION; RIBONUCLEIC ACID; RIBONUCLEIC ACIDS; ROOM TEMPERATURE; SAMPLE HANDLING; SAMPLE STORAGE; TRANSFER RNA;

ACIDS; DESORPTION; ENZYMES; HEAVY WATER; ISOTOPES; MASS SPECTROMETRY; NUCLEIC ACIDS; RNA;

PH EFFECTS;

ENDONUCLEASE; ENZYME; ISOTOPE; OXYGEN; OXYGEN 18; RIBONUCLEASE; RIBONUCLEASE T1; RNA; BACTERIAL RNA; GLUTAMIC ACID TRANSFER RNA; HYDROLASE; OLIGORIBONUCLEOTIDE; WATER;

3' UNTRANSLATED REGION; ACCURACY; ACIDITY; ALKALINITY; ARTICLE; BACK EXCHANGE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DIGESTION; ENZYME PURIFICATION; INCUBATION TIME; ION EXCHANGE; ISOTOPE LABELING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PH; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; ROOM TEMPERATURE; TEMPERATURE; BIOCATALYSIS; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; TIME;

BIOCATALYSIS; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; HYDROLASES; ISOTOPE LABELING; OLIGORIBONUCLEOTIDES; OXYGEN ISOTOPES; RIBONUCLEASE T1; RNA, BACTERIAL; RNA, TRANSFER, GLU; TEMPERATURE; TIME FACTORS; WATER;

EID: 70349109653     PISSN: 10765174     EISSN: 10969888     Source Type: Journal    
DOI: 10.1002/jms.1596     Document Type: Article

References (14)

1. C. Ding, C. R. Cantor. Quantitative analysis of nucleic acids - the last few years of progress. Journal of Biochemistry and Molecular Biology 2004, 37, 1.
2. J. Liu, P. A. Feldman, J. S. Lippy, E. Bobkova, M. G. Kurilla, T. D. Y. Chung. A scintillation proximity assay for RNA detection. Analytical Biochemistry 2001, 289, 239.
3. C. Payan, N. Veal, B. Crescenzo-Chaigne, L. Belec, J. Pillot. New quantitative assay of hepatitis B and C viruses by competitive PCR using alternative internal sequences. Journal of Virological Methods 1997, 65, 299.
4. S. Ro, C. Park, J. Jin, K. M. Sanders, W. Yan. A PCR-based method for detection and quantification of small RNAs. Biochemical and Biophysical Research Communications 2006, 351, 756.
5. S. Grove, R. Faller, K. B. Soleim, B. H. Dannevig. Absolute quantitation of RNA by a competitive real-time RT-PCR method using piscine nodavirus as a model. Journal of Virological Methods 2006, 2006, 104.
6. B. Elsholz, R. Worl, L. Blohn, J. Albers, H. Feucht, T. Grunwald, B. Jurgen, T. Schweder, R. Hintsche. Automated detection and quantitation of bacterial RNA by using electrical microarrays. Analytical Chemistry 2006, 78, 4794.
7. Z. Meng, P. A. Limbach. Quantitation of ribonucleic acids using 18O labeling and mass spectrometry. Analytical Chemistry 2005, 77, 1891.
8. B. T. Berhane, P. A. Limbach. Stable isotope labeling for matrix-assisted laser desorption/ionization mass spectrometry and postsource decay of analysis of ribonucleic acids. Journal of Mass Spectrometry 2003, 38, 872.
9. 18O labeling for comparative proteomics: evaluation of endoprotease Glu-C as the catalytic agent. Journal of Proteome Research 2002, 1, 27.
10. 18O labeling for comparative proteomics: model studies with two serotypes of adenovirus. Analytical Chemistry 2001, 73, 2836.
11. 18Olabeled amino acids for use as internal standards with mass spectrometry. Biomedical Mass Spectrometry 1979, 6, 309.
12. 16O quantitative proteomics experiments by incorporation of immobilized trypsin into the initial digestion step. Analytical Chemistry 2007, 79, 2158.
13. A. Apffel, J. A. Chakel, S. Fischer, K. Lichtenwalter, W. S. Hancock. Analysis of oligonucleotides by HPLC-electrospray ionization mass spectrometry. Analytical Chemistry 1997, 69, 1320.
14. W. D. Dominick, B. T. Berhane, J. S. Mecomber, P. A. Limbach. Covalent immobilization of proteases and nucleases to poly(methylmethacrylate). Analytical and Bioanalytical Chemistry 2003, 376, 349.