Immunohistochemical study of the autophagy marker microtubule-associated protein 1 light chain 3 in normal and steatotic human livers

Hepatology Research

Volumn 44, Issue 7, 2014, Pages 779-787

  Kashima, Jumpei ^a   Shintani Ishida, Kaori ^a   Nakajima, Makoto ^a   Maeda, Hideyuki ^a   Unuma, Kana ^b   Uchiyama, Yasuo ^c   Yoshida, Ken Ichi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^c JUNTENDO UNIVERSITY   (Japan)

Author keywords

Adipose differentiation related protein; Autophagy; Lipid droplet; Microtubule associated protein 1 light chain 3; Steatosis

Indexed keywords

ADIPOPHILIN; MICROTUBULE ASSOCIATED PROTEIN 1;

ADULT; AGED; ARTICLE; AUTOPHAGOSOME; AUTOPHAGY; AUTOPSY; CONTROLLED STUDY; FEMALE; HUMAN; HUMAN TISSUE; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOHISTOCHEMISTRY; MALE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; STEATOSIS; TURNOVER TIME; VERY ELDERLY;

EID: 84903903918     PISSN: 13866346     EISSN: 1872034X     Source Type: Journal    
DOI: 10.1111/hepr.12183     Document Type: Article

References (22)

1. Yin X-M, Ding W-X, Gao W. Autophagy in the liver. Hepatology 2008; 47: 1773-1785.
2. Mizushima N, Yoshimori T, Levine B. Methods in mammalian autophagy research. Cell 2010; 140: 313-326.
3. Komatsu M, Waguri S, Ueno T etal. Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol 2005; 169: 425-434.
4. Komatsu M, Waguri S, Koike M etal. Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell 2007; 131: 1149-1163.
5. Martinet W, De Meyer GR, Andries L, Herman AG, Kockx MM. Detection of autophagy in tissue by standard immunohistochemistry: possibilities and limitations. Autophagy 2006; 2: 55-57.
6. Martin S, Parton RG. Lipid droplets: a unified view of a dynamic organelle. Nat Rev Mol Cell Biol 2006; 7: 373-378.
7. Chang BH, Chan L. Regulation of triglyceride metabolism. III. Emerging role of lipid droplet protein ADFP in health and disease. Am J Physiol Gastrointest Liver Physiol 2007; 292: G1465-1468.
8. Singh R, Kaushik S, Wang Y etal. Autophagy regulates lipid metabolism. Nature 2009; 458: 1131-1135.
9. Czaja MJ. Autophagy in health and disease. 2. Regulation of lipid metabolism and storage by autophagy: pathophysiological implications. Am J Physiol Cell Physiol 2010; 298: C973-978.
10. Shibata M, Yoshimura K, Furuya N etal. The MAP1-LC3 conjugation system is involved in lipid droplet formation. Biochem Biophys Res Commun 2009; 382: 419-423.
11. Straub BK, Stoeffel P, Heid H, Zimbelmann R, Schirmacher P. Differential pattern of lipid droplet-associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 2008; 47: 1936-1946.
12. Mak KM, Ren C, Ponomarenko A, Cao Q, Lieber CS. Adipose differentiation-related protein is a reliable lipid droplet marker in alcoholic fatty liver of rats. Alcohol Clin Exp Res 2008; 32: 683-689.
13. Motomura W, Inoue M, Ohtake T etal. Up-regulation of ADRP in fatty liver in human and liver steatosis in mice fed with high fat diet. Biochem Biophys Res Commun 2006; 340: 1111-1118.
14. Ito T, Nobutomo K, Fujimiya T, Yoshida K. Importance of explanation before and after forensic autopsy to the bereaved family: lessons from a questionnaire study. J Med Ethics 2010; 36: 103-105.
15. David EK, Elizabeth MB, Mark VN etal. Design and validation of a histological scoring system for nonalcoholic fatty liver disease. Hepatology 2005; 41: 1313-1321.
16. Shintaku M. Immunohistochemical localization of autophagosomal membrane-associated protein LC3 in granular cell tumor and schwannoma. Virchows Arch 2011; 459: 315-319.
17. Jiang ZF, Shao LJ, Wang WM, Yan XB, Liu RY. Decreased expression of Beclin-1 and LC3 in human lung cancer. Mol Biol Rep 2012; 39: 259-267.
18. Rosenfeldt MT, Nixon C, Liu E, Mah LY, Ryan KM. Analysis of macroautophagy by immunohistochemistry. Autophagy 2012; 8: 963-969.
19. Zhang C, Cuervo AM. Restoration of chaperone-mediated autophagy in aging lover improves cellular maintenance and hepatic function. Nat Med 2008; 14: 959-965.
20. Rautou PE, Mansouri A, Lebrec D, Durand F, Valla D, Moreau R. Autophagy in liver diseases. J Hepatol 2010; 53: 1123-1134.
21. Unuma K, Aki T, Matsuda S, Funakoshi T, Yoshida K, Uemura K. Inducer of heme oxygenase-1 cobalt protoporphyrin accelerates autophagy and suppresses oxidative damages during lipopolysaccharide treatment in rat liver. Hepatol Res 2013; 43: 91-96.
22. Tessari P, Coracina A, Cosma A, Tiengo A. Hepatic lipid metabolism and non-alcoholic fatty liver disease. Nutr Metab Cardiovasc Dis 2009; 19: 291-302.