Quantitative, label-free characterization of stem cell differentiation at the single-cell level by broadband coherent anti-stokes raman scattering microscopy

Tissue Engineering - Part C: Methods

Volumn 20, Issue 7, 2014, Pages 562-569

  Lee, Young Jong ^a   Vega, Sebastián L ^b   Patel, Parth J ^c   Aamer, Khaled A ^a   Moghe, Prabhas V ^b,c   Cicerone, Marcus T ^a  

^a NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

^b The State University of New Jersey   (United States)

^c RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COHERENT SCATTERING; CYTOLOGY; RAMAN SCATTERING; RAMAN SPECTROSCOPY; STEM CELLS;

COHERENT ANTI-STOKES RAMAN SCATTERING; COHERENT ANTI-STOKES RAMAN SCATTERING MICROSCOPY; HIGH SPATIAL RESOLUTION; HUMAN MESENCHYMAL STEM CELLS; HYPER-SPECTRAL IMAGES; MULTI VARIATE ANALYSIS; POPULATION HETEROGENEITY; STEM CELL DIFFERENTIATION;

OSTEOBLASTS;

BIOLOGICAL MARKER; LIPID; MINERAL;

ADIPOCYTE; ARTICLE; BROADBAND COHERENT ANTI STOKES RAMAN SCATTERING MICROSCOPY; CELL COUNT; CELL DIFFERENTIATION; CELL NUCLEUS; CYTOSOL; HUMAN; HUMAN CELL; MESENCHYMAL STEM CELL; MICROSCOPY; OSTEOBLAST; PHENOTYPE; PRIORITY JOURNAL; CELL LINE; CYTOLOGY; IMAGE PROCESSING; MESENCHYMAL STROMA CELL; METABOLISM; PROCEDURES; RAMAN SPECTROMETRY;

ADIPOCYTES; CELL DIFFERENTIATION; CELL LINE; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MESENCHYMAL STROMAL CELLS; MICROSCOPY; OSTEOBLASTS; SPECTRUM ANALYSIS, RAMAN;

EID: 84903778853     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2013.0472     Document Type: Article

References (33)

1. Gentleman, E., Swain, R.J., Evans, N.D., Boonrungsiman, S., Jell, G., Ball, M.D., et al. Comparative materials differences revealed in engineered bone as a function of cell-specific differentiation. Nat Mater 8, 763, 2009
2. James, D., Nam, H., Seandel, M., Nolan, D., Janovitz, T., Tomishima, M., et al. Expansion and maintenance of human embryonic stem cell-derived endothelial cells by TGFb inhibition is Id1 dependent. Nat Biotechnol 28, 161, 2010
3. Shafa, M., Sjonnesen, K., Yamashita, A., Liu, S., Michalak, M., Kallos, M.S., et al. Expansion and long-Term maintenance of induced pluripotent stem cells in stirred suspension bioreactors. J Tissue Eng Regen Med 6, 462, 2012
4. Navin, N., Kendall, J., Troge, J., Andrews, P., Rodgers, L., McIndoo, J., et al. Tumour evolution inferred by single-cell sequencing. Nature 472, 90, 2011
5. Notingher, I., Jell, G., Lohbauer, U., Salih, V., and Hench, L.L. In situ non-invasive spectral discrimination between bone cell phenotypes used in tissue engineering. J Cell Biochem 92, 1180, 2004
6. Chan, J.W., Lieu, D.K., Huser, T., and Li, R.A. Label-free separation of human embryonic stem cells and their cardiac derivatives using Raman spectroscopy. Anal Chem 81, 1324, 2009
7. Ami, D., Neri, T., Natalello, A., Mereghetti, P., Doglia, S.M., Zanoni, M., et al. Embryonic stem cell differentiation studied by FT-IR spectroscopy. Biochim Biophys Acta 1783, 98, 2008
8. Lonergan, T., Brenner, C., and Bavister, B. Differentiationrelated changes in mitochondrial properties as indicators of stem cell competence. J Cell Physiol 208, 149, 2006
9. Varum, S., Rodrigues, A.S., Moura, M.B., Momcilovic, O., Ramalho-Santos, J., Van Houten, B., et al. Energy metabolism in human pluripotent stem cells and their differentiated counterparts. Plos One 6, e20914, 2011
10. Treiser, M.D., Yang, E.H., Gordonov, S., Cohen, D.M., Androulakis, I.P., Kohn, J., et al. Cytoskeleton-based forecasting of stem cell lineage fates. Proc Natl Acad Sci U S A 107, 610, 2010
11. Chan, J.W., and Lieu, D.K. Label-free biochemical characterization of stem cells using vibrational spectroscopy. J Biophotonics 2, 656, 2009
12. Notingher, I., Bisson, I., Bishop, A.E., Randle, W.L., Polak, J.M.P., and Hench, L.L. In situ spectral monitoring of mRNA translation in embryonic stem cells during differentiation in vitro. Anal Chem 76, 3185, 2004
13. Chiang, H.K., Peng, F-Y., Hung, S-C., and Feng, Y-C. In situ Raman spectroscopic monitoring of hydroxyapatite as human mesenchymal stem cells differentiate into osteoblasts. J Raman Spectrosc 40, 546, 2009
14. Kunstar, A., Leijten, J., van Leuveren, S., Hilderink, J., Otto, C., van Blitterswijk, C.A., et al. Recognizing different tissues in human fetal femur cartilage by label-free Raman microspectroscopy. J Biomed Opt 17, 116012, 2012
15. Zumbusch, A., Holtom, G.R., and Xie, X.S. Three-dimensional vibrational imaging by coherent anti-stokes Raman scattering. Phys Rev Lett 82, 4142, 1999
16. Evans, C.L., and Xie, X.S. Coherent anti-stokes Raman scattering microscopy: Chemical imaging for biology and medicine. Annu Rev Anal Chem 1, 883, 2008
17. Konorov, S.O., Glover, C.H., Piret, J.M., Bryan, J., Schulze, H.G., Blades, M.W., et al. In situ analysis of living embryonic stem cells by coherent anti-stokes Raman microscopy. Anal Chem 79, 7221, 2007
18. Downes, A., Mouras, R., Bagnaninchi, P., and Elfick, A. Raman spectroscopy and CARS microscopy of stem cells and their derivatives. J Raman Spectrosc 42, 1864, 2011
19. Kee, T.W., and Cicerone, M.T. Simple approach to one-laser, broadband coherent anti-stokes Raman scattering microscopy. Opt Lett 29, 2701, 2004
20. Kano, H., and Hamaguchi, H. Femtosecond coherent anti-Stokes Raman scattering spectroscopy using supercontinuum generated from a photonic crystal fiber. Appl Phys Lett 85, 4298, 2004
21. Lee, Y.J., Moon, D., Migler, K.B., and Cicerone, M.T. Quantitative image analysis of broadband CARS hyperspectral images of polymer blends. Anal Chem 83, 2733, 2011
22. Parekh, S.H., Lee, Y.J., Aamer, K.A., and Cicerone, M.T. Label-free cellular imaging by broadband coherent antistokes Raman scattering microscopy. Biophys J 99, 2695, 2010
23. Afonso, P.V., Janka-Junttila, M., Lee, Y.J., McCann, C.P., Oliver, C.M., Aamer, K.A., et al. LTB4 is a signal-relay molecule during neutrophil chemotaxis. Dev Cell 22, 1079, 2012
24. Kano, H., and Hamaguchi, H. Supercontinuum dynamically visualizes a dividing single cell. Anal Chem 79, 8967, 2007
25. Bonn, M., Müller, M., Rinia, H.A., and Burger, K.N.J. Imaging of chemical and physical state of individual cellular lipid droplets using multiplex CARS microscopy. J Raman Spectrosc 40, 763, 2009
26. Sisan, D.R., Halter, M., Hubbard, J.B., and Plant, A.L. Predicting rates of cell state change caused by stochastic fluctuations using a data-driven landscape model. Proc Natl Acad Sci U S A 109, 19262, 2012
27. Pully, V.V., Lenferink, A., and Otto, C. Raman-fluorescence hybrid microspectroscopy of cell nuclei. Vib Spectrosc 53, 12, 2010
28. Bräutigam, K., Bocklitz, T., Schmitt, M., Rösch, P., and Popp, J. Raman spectroscopic imaging for the real-Time detection of chemical changes associated with docetaxel exposure. Chem Phys Chem 14, 550, 2013
29. Zuser, E., Chernenko, T., Newmark, J., Miljković, M., and Diem, M. Confocal Raman microspectral imaging (CRMI) of murine stem cell colonies. Analyst 135, 3030, 2010
30. Pascut, F.C., Goh, H.T., George, V., Denning, C., and Notingher, I. Toward label-free Raman-Activated cell sorting of cardiomyocytes derived from human embryonic stem cells. J Biomed Opt 16, 045002, 2011
31. Liu, Y., Lee, Y.J., and Cicerone, M.T. Broadband CARS spectral phase retrieval using a time-domain Kramers-Kronig transform. Opt Lett 34, 1363, 2009
32. Pittenger, M.F., Mackay, A.M., Beck, S.C., Jaiswal, R.K., Douglas, R., Mosca, J.D., et al. Multilineage potential of adult human mesenchymal stem cells. Science 284, 143, 1999
33. Notingher, L., Jell, G., Notingher, P.L., Bisson, I., Tsigkou, O., Polak, J.M., et al. Multivariate analysis of Raman spectra for in vitro non-invasive studies of living cells. J Mol Struct 744, 179, 2005 Bioengineering