Live cell imaging of in vitro human trophoblast syncytialization

Biology of Reproduction

Volumn 90, Issue 6, 2014, Pages

  Wang, Rui ^a,b   Dang, Yan Li ^c   Zheng, Ru ^a,b   Li, Yue ^a   Li, Weiwei ^a,d   Lu, Xiaoyin ^a,b   Wang, Li Juan ^a   Zhu, Cheng ^a   Lin, Hai Yan ^a   Wang, Hongmei ^a  

^a INSTITUTE OF ZOOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c 306 HOSPITAL OF PLA   (China)

^d BEIJING NORMAL UNIVERSITY   (China)

Author keywords

Bewo; Developmental biology; Live imaging; Placental development; Placentation; Pregnancy; Primary cultured cytotrophoblast cells; Syncytialization; Syncytiotrophoblast; Trophoblast

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; HOE 33342; UVOMORULIN; BENZIMIDAZOLE DERIVATIVE; FLUORESCENT DYE; FORSKOLIN; IMMUNOLOGICAL ADJUVANT;

ARTICLE; CAMERA; CELL FUSION; CELL MEMBRANE; CELL POPULATION; CHORIOCARCINOMA CELL LINE; CYTOPLASM; CYTOTROPHOBLAST; FEMALE; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMAGING AND DISPLAY; IMAGING SYSTEM; IMMUNOFLUORESCENCE; IN VITRO STUDY; LIVE CELL IMAGING; MICROSCOPE; MULTINUCLEAR CELL; PLASMID; PRIORITY JOURNAL; SYNCYTIALIZATION; SYNCYTIUM; TROPHOBLAST; CHORIOCARCINOMA; CYTOLOGY; DRUG EFFECTS; PATHOLOGY; PHYSIOLOGY; PREGNANCY; PRIMARY CELL CULTURE; PROCEDURES; REPRODUCTION; TUMOR CELL LINE; UTERUS CANCER; VIDEO MICROSCOPY;

ADJUVANTS, IMMUNOLOGIC; BENZIMIDAZOLES; CELL FUSION; CELL LINE, TUMOR; CHORIOCARCINOMA; COLFORSIN; CYTOPLASM; FEMALE; FLUORESCENT DYES; HUMANS; MEMBRANE MICRODOMAINS; MICROSCOPY, FLUORESCENCE; MICROSCOPY, VIDEO; PLACENTATION; PREGNANCY; PRIMARY CELL CULTURE; TROPHOBLASTS; UTERINE NEOPLASMS;

EID: 84903760173     PISSN: 00063363     EISSN: 15297268     Source Type: Journal    
DOI: 10.1095/biolreprod.113.114892     Document Type: Article

References (47)

1. Cross JC, Werb Z, Fisher SJ. Implantation and the placenta: key pieces of the development puzzle. Science 1994; 266:1508-1518.
2. Fu J, Lv X, Lin H, Wu L, Wang R, Zhou Z, Zhang B, Wang YL, Tsang BK, Zhu C, Wang H. Ubiquitin ligase cullin 7 induces epithelialmesenchymal transition in human choriocarcinoma cells. J Biol Chem 2010; 285:10870-10879.
3. Potgens AJ, Schmitz U, Bose P, Versmold A, Kaufmann P, Frank HG. Mechanisms of syncytial fusion: a review. Placenta 2002; 23(suppl A): S107-113.
4. Huppertz B, Meiri H, Gizurarson S, Osol G, Sammar M. Placental protein 13 (PP13): a new biological target shifting individualized risk assessment to personalized drug design combating pre-eclampsia. Hum Reprod Update 2013; 19:391-405.
5. Rhodin JA, Terzakis J. The ultrastructure of the human full-term placenta. J Ultrastruct Res 1962; 6:88-106.
6. Terzakis JA. The ultrastructure of normal human first trimester placenta. J Ultrastruct Res 1963; 9:268-284.
7. Jones CJ, Fox H. Ultrastructure of the normal human placenta. Electron Microsc Rev 1991; 4:129-178.
8. Boyd JD, Hamilton WJ, Boyd CA. The surface of the syncytium of the human chorionic villus. J Anat 1968; 102:553-563.
9. Boyd JD, Hamilton WJ. Electron microscopic observations on the cytotrophoblast contribution to the syncytium in the human placenta. J Anat 1966; 100:535-548.
10. Huppertz B, Bartz C, Kokozidou M. Trophoblast fusion: fusogenic proteins, syncytins and ADAMs, and other prerequisites for syncytial fusion. Micron 2006; 37:509-517.
11. Pattillo RA, Gey GO, Delfs E, Mattingly RF. Human hormone production in vitro. Science 1968; 159:1467-1469.
12. Wice B, Menton D, Geuze H, Schwartz AL. Modulators of cyclic AMP metabolism induce syncytiotrophoblast formation in vitro. Exp Cell Res 1990; 186:306-316.
13. Kliman HJ, Nestler JE, Sermasi E, Sanger JM, Strauss JF, 3rd. Purification, characterization, and in vitro differentiation of cytotrophoblasts from human term placentae. Endocrinology 1986; 118:1567-1582.
14. Baczyk D, Dunk C, Huppertz B, Maxwell C, Reister F, Giannoulias D, Kingdom JC. Bi-potential behaviour of cytotrophoblasts in first trimester chorionic villi. Placenta 2006; 27:367-374.
15. Isaksen DE, Liu NJ, Weisblat DA. Inductive regulation of cell fusion in leech. Development 1999; 126:3381-3390.
16. Forest CL. Genetic control of plasma membrane adhesion and fusion in Chlamydomonas gametes. J Cell Sci 1987; 88(pt 5):613-621.
17. Glass NL, Rasmussen C, Roca MG, Read ND. Hyphal homing, fusion and mycelial interconnectedness. Trends Microbiol 2004; 12:135-141.
18. Podbilewicz B, White JG. Cell fusions in the developing epithelial of C. elegans. Dev Biol 1994; 161:408-424.
19. Chen EH. Invasive podosomes and myoblast fusion. Curr Top Membr 2011; 68:235-258.
20. Loutit JF, Nisbet NW. The origin of osteoclasts. Immunobiology 1982; 161:193-203.
21. Chambers TJ. Multinucleate giant cells. J Pathol 1978; 126:125-148.
22. Kahn AJ, Teitelbaum SL, Malone JD, Krukowski M. The relationship of monocytic cells to the differentiation and resorption of bone. Prog Clin Biol Res 1982; 110(pt B):239-248.
23. Vignery A. Osteoclasts and giant cells: macrophage-macrophage fusion mechanism. Int J Exp Pathol 2000; 81:291-304.
24. Gattegno T, Mittal A, Valansi C, Nguyen KC, Hall DH, Chernomordik LV, Podbilewicz B. Genetic control of fusion pore expansion in the epidermis of Caenorhabditis elegans. Mol Biol Cell 2007; 18:1153-1166.
25. Sens KL, Zhang S, Jin P, Duan R, Zhang G, Luo F, Parachini L, Chen EH. An invasive podosome-like structure promotes fusion pore formation during myoblast fusion. J Cell Biol 2010; 191:1013-1027.
26. Mohler WA, Shemer G, del Campo JJ, Valansi C, Opoku-Serebuoh E, Scranton V, Assaf N, White JG, Podbilewicz B. The type I membrane protein EFF-1 is essential for developmental cell fusion. Dev Cell 2002; 2: 355-362.
27. Podbilewicz B, Leikina E, Sapir A, Valansi C, Suissa M, Shemer G, Chernomordik LV. The C. elegans developmental fusogen EFF-1 mediates homotypic fusion in heterologous cells and in vivo. Dev Cell 2006; 11:471-481.
28. Oren-Suissa M, Hall DH, Treinin M, Shemer G, Podbilewicz B. The fusogen EFF-1 controls sculpting of mechanosensory dendrites. Science 2010; 328:1285-1288.
29. Sapir A, Choi J, Leikina E, Avinoam O, Valansi C, Chernomordik LV, Newman AP, Podbilewicz B. AFF-1, a FOS-1-regulated fusogen, mediates fusion of the anchor cell in C. elegans. Developmental Cell 2007; 12:683-698.
30. Borges M, Bose P, Frank HG, Kaufmann P, Potgens AJ. A two-colour fluorescence assay for the measurement of syncytial fusion between trophoblast-derived cell lines. Placenta 2003; 24:959-964.
31. Zhou Z, Zhang Q, Lu X, Wang R, Wang H, Wang YL, Zhu C, Lin HY, Wang H. The proprotein convertase furin is required for trophoblast syncytialization. Cell Death Dis 2013; 4:e593.
32. Guilbert LJ, Winkler-Lowen B, Sherburne R, Rote NS, Li H, Morrish DW. Preparation and functional characterization of villous cytotrophoblasts free of syncytial fragments. Placenta 2002; 23:175-183.
33. Lin FY, Chang CW, Cheong ML, Chen HC, Lee DY, Chang GD, Chen H. Dual-specificity phosphatase 23 mediates GCM1 dephosphorylation and activation. Nucleic Acids Res 2011; 39:848-861.
34. Redman CW, Sargent IL. Placental stress and pre-eclampsia: a revised view. Placenta 2009; 30(suppl A):S38-42.
35. Huppertz B. Placental origins of preeclampsia: challenging the current hypothesis. Hypertension 2008; 51:970-975.
36. Young BC, Levine RJ, Karumanchi SA. Pathogenesis of preeclampsia. Annu Rev Pathol 2010; 5:173-192.
37. Chen CP, Chen CY, Yang YC, Su TH, Chen H. Decreased placental GCM1 (glial cells missing) gene expression in pre-eclampsia. Placenta 2004; 25:413-421.
38. Chen CP, Wang KG, Chen CY, Yu C, Chuang HC, Chen H. Altered placental syncytin and its receptor ASCT2 expression in placental development and pre-eclampsia. BJOG 2006; 113:152-158.
39. Gauster M, Moser G, Orendi K, Huppertz B. Factors involved in regulating trophoblast fusion: potential role in the development of preeclampsia. Placenta 2009; 30(suppl A):S49-54.
40. Kesper DA, Stute C, Buttgereit D, Kreiskother N, Vishnu S, Fischbach KF, Renkawitz-Pohl R. Myoblast fusion in Drosophila melanogaster is mediated through a fusion-restricted myogenic-adhesive structure (FuRMAS). Dev Dyn 2007; 236:404-415.
41. Linder S, Wiesner C, Himmel M. Degrading devices: invadosomes in proteolytic cell invasion. Annu Rev Cell Dev Biol 2011; 27:185-211.
42. Lindau M, Almers W. Structure and function of fusion pores in exocytosis and ectoplasmic membrane fusion. Curr Opin Cell Biol 1995; 7:509-517.
43. Hickey PC, Jacobson D, Read ND, Glass NL. Live-cell imaging of vegetative hyphal fusion in Neurospora crassa. Fungal Genet Biol 2002; 37:109-119.
44. Sarkar DP, Morris SJ, Eidelman O, Zimmerberg J, Blumenthal R. Initial stages of influenza hemagglutinin-induced cell fusion monitored simultaneously by two fluorescent events: cytoplasmic continuity and lipid mixing. J Cell Biol 1989; 109:113-122.
45. Jin P, Duan R, Luo F, Zhang G, Hong SN, Chen EH. Competition between Blown fuse and WASP for WIP binding regulates the dynamics of WASP-dependent actin polymerization in vivo. Dev Cell 2011; 20: 623-638.
46. Duan R, Jin P, Luo F, Zhang G, Anderson N, Chen EH. Group I PAKs function downstream of Rac to promote podosome invasion during myoblast fusion in vivo. J Cell Biol 2012; 199:169-185.
47. Gruenbaum-Cohen Y, Harel I, Umansky KB, Tzahor E, Snapper SB, Shilo BZ, Schejter ED. The actin regulator N-WASp is required for muscle-cell fusion in mice. Proc Natl Acad Sci U S A 2012; 109:11211-11216.