An optimized protocol for isolating primary epithelial cell chromatin for ChIP

PLoS ONE

Volumn 9, Issue 6, 2014, Pages

  Browne, James A ^a,b   Harris, Ann ^a,b   Leir, Shih Hsing ^a,b  

^a Lurie Children's Research Center   (United States)

^b NORTHWESTERN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BUFFER; DETERGENT; DYE; EDETIC ACID; GREY BLUE; ICE; METHYL GREEN; METHYL GREEN PYRONINE; PYRONINE; TRITON X 100; TRYPSIN; UNCLASSIFIED DRUG; CHROMATIN;

AGITATION; ARTICLE; CELL ADHESION; CELL ISOLATION; CELL LINE; CELL MEMBRANE; CELL NUCLEUS; CELL SUSPENSION; CELL TYPE; CHROMATIN; CHROMATIN IMMUNOPRECIPITATION; CYTOLYSIS; CYTOPLASM; EQUILIBRIUM CONSTANT; HUMAN; HUMAN CELL; INCUBATION TIME; LUNG ALVEOLUS EPITHELIUM; MICROSCOPY; NEEDLE; PROCESS OPTIMIZATION; REPRODUCIBILITY; ROOM TEMPERATURE; EPITHELIUM CELL; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES;

CELL LINE; CHROMATIN; CHROMATIN IMMUNOPRECIPITATION; EPITHELIAL CELLS; HUMANS;

EID: 84903648122     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0100099     Document Type: Article

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