Quantitative HPLC-MS analysis of nucleotide sugars in plant cells following off-line SPE sample preparation

Analytical and Bioanalytical Chemistry

Volumn 406, Issue 13, 2014, Pages 3229-3237

  Behmüller, Robert ^a   Forstenlehner, Ines C ^a   Tenhaken, Raimund ^a   Huber, Christian G ^a  

^a UNIVERSITY OF SALZBURG   (Austria)

Author keywords

Arabidopsis thaliana; Electrospray ionization mass spectrometry; Liquid chromatography; Off line solid phase extraction; Porous graphitic carbon; UDP sugars; Ugd2,3 mutant

Indexed keywords

CALIBRATION; CARBON; ELECTROSPRAY IONIZATION; GLUCOSE; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; PHASE SEPARATION; PHYSIOLOGY; POROUS MATERIALS; REGRESSION ANALYSIS;

ARABIDOPSIS THALIANA; ELECTROSPRAY IONIZATION MASS SPECTROMETRY; POROUS GRAPHITIC CARBON; SOLID-PHASE EXTRACTION; UDP-SUGARS; UGD2,3 MUTANT;

EXTRACTION;

ARABIDOPSIS THALIANA;

URIDINE DIPHOSPHATE SUGARS;

ARABIDOPSIS; CHEMISTRY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GENETICS; ISOLATION AND PURIFICATION; LIQUID-LIQUID EXTRACTION; METHODS; MUTATION; PLANTS, GENETICALLY MODIFIED; SEEDS; SOLID PHASE EXTRACTION; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

ARABIDOPSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; LIQUID-LIQUID EXTRACTION; MUTATION; PLANTS, GENETICALLY MODIFIED; SEEDS; SOLID PHASE EXTRACTION; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; URIDINE DIPHOSPHATE SUGARS;

EID: 84903623968     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-014-7746-3     Document Type: Article

References (21)

1. Kotake T, Hirosawa C, Ando Y, Tsumuraya Y (2010) Generation of nucleotide sugars for biomass formation in plants. Plant Biotechnology 27(3):231-236
2. Sharples SC, Fry SC (2007) Radioisotope ratios discriminate between competing pathways of cell wall polysaccharide and RNA biosynthesis in living plant cells. Plant J 52(2):252-262 (Pubitemid 47537577)
3. Bar-Peled M, O'Neill MA (2011) Plant nucleotide sugar formation, interconversion, and salvage by sugar recycling.Annu Rev Plant Biol 62:127-155
4. Kotake T, Hojo S, Tajima N, Matsuoka K, Koyama T, Tsumuraya Y (2008) A bifunctional enzyme with L-fucokinase and Gdp-L-fucose pyrophosphorylase activities salvages free L-fucose in Arabidopsis. J Biol Chem 283(13):8125-8135
5. Reboul R, Geserick C, Pabst M, Frey B, Wittmann D, Lutz-Meindl U, Leonard R, Tenhaken R (2011) Down-regulation of UDPglucuronic acid biosynthesis leads to swollen plant cell walls and severe developmental defects associated with changes in pectic polysaccharides. J Biol Chem 286(46):39982-39992
6. Reboul R, Tenhaken R (2012) An emerging role of pectic rhamnogalacturonanii for cell wall integrity. Plant Signal Behav 7(2):298-299
7. TolstikovVV, FiehnO (2002) Analysis of highly polar compounds of plant origin: combination of hydrophilic interaction chromatography and electrospray ion trap mass spectrometry. Anal Biochem 301(2): 298-307 (Pubitemid 34158194)
8. Rabina J, Maki M, Savilahti EM, Jarvinen N, Penttila L, Renkonen R (2001) Analysis of nucleotide sugars from cell lysates by ion-pair solid-phase extraction and reversed-phase high-performance liquid chromatography. Glycoconj J 18(10):799-805 (Pubitemid 36074981)
9. Pabst M, Grass J, Fischl R, Leonard R, Jin C, Hinterkorner G, Borth N, Altmann F (2010) Nucleotide and nucleotide sugar analysis by liquid chromatography-electrospray ionization-mass spectrometry on surface-conditioned porous graphitic carbon. Anal Chem 82(23): 9782-9788
10. Alonso AP, Piasecki RJ,WangY, LaClair RW, Shachar-HillY (2010) Quantifying the labeling and the levels of plant cell wall precursors using ion chromatography tandem mass spectrometry. Plant Physiol 153(3):915-924
11. Lunn JE, Feil R, Hendriks JH, Gibon Y, Morcuende R, Osuna D, ScheibleWR, Carillo P, HajirezaeiMR, StittM(2006) Sugar-induced increases in trehalose 6-phosphate are correlated with redox activation of adpglucose pyrophosphorylase and higher rates of starch synthesis in Arabidopsis thaliana. Biochem J 397(1):139-148 (Pubitemid 44032845)
12. Pabst M, Altmann F (2008) Influence of electrosorption, solvent, temperature, and ion polarity on the performance of LC-ESI-MS using graphitic carbon for acidic oligosaccharides. Anal Chem 80(19):7534-7542
13. Tornkvist A, Nilsson S, Amirkhani A, Nyholm LM, Nyholm L (2004) Interference of the electrospray voltage on chromatographic separations using porous graphitic carbon columns. JMass Spectrom 39(2):216-222 (Pubitemid 38292498)
14. Melmer M, Stangler T, Premstaller A, Lindner W (2010) Effects of the redox state of porous graphitic carbon on the retention of oligosaccharides. J Chromatogr A 1217(39):6097-6101
15. Jozefczuk S, Klie S, Catchpole G, Szymanski J, Cuadros-Inostroza A, Steinhauser D, Selbig J, Willmitzer L (2010) Metabolomic and transcriptomic stress response of escherichia coli. Mol Syst Biol 6: 364
16. Weckwerth W (2003) Metabolomics in systems biology. Annu Rev Plant Biol 54:669-689 (Pubitemid 37400009)
17. Sansone SA, Fan T, Goodacre R, Griffin JL, Hardy NW, Kaddurah-Daouk R, Kristal BS, Lindon J, Mendes P, Morrison N, Nikolau B, Robertson D, Sumner LW, Taylor C, van derWerf M, van Ommen B, Fiehn O (2007) The metabolomics standards initiative. Nat Biotechnol 25(8):846-848
18. Kusano M, Fukushima A, Redestig H, Saito K (2011) Metabolomic approaches toward understanding nitrogen metabolism in plants. J Exp Bot 62(4):1439-1453
19. Miller JN,Miller JC (2005) Statistics and chemometrics for analytical chemistry, 5th edn. Pearson, Harlow
20. Reiter WD (2008) Biochemical genetics of nucleotide sugar interconversion reactions. Curr Opin Plant Biol 11(3):236-243
21. Klinghammer M, Tenhaken R (2007) Genome-wide analysis of the Udp-glucose dehydrogenase gene family in Arabidopsis, a Key enzyme for matrix polysaccharides in cell walls. J Exp Bot 58(13): 3609-3621 (Pubitemid 350232996)