A new regulator of pathogenicity (bvlR) is required for full virulence and tight microcolony formation in Pseudomonas aeruginosa

Microbiology (United Kingdom)

Volumn 160, Issue PART 7, 2014, Pages 1488-1500

  McCarthy, Ronan R ^a   Mooij, Marlies J ^a,d   Reen, F Jerry ^a   Lesouhaitier, Olivier ^b   O'Gara, Fergal ^a,c  

^a UNIVERSITY COLLEGE CORK   (Ireland)

^b UNIVERSITÉ DE ROUEN   (France)

^c CURTIN UNIVERSITY   (Australia)

^d MAASTRICHT UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

EXOTOXIN; SPACER DNA;

ANIMAL EXPERIMENT; ARTICLE; BACTERIAL VIRULENCE; CAENORHABDITIS ELEGANS; COLONY FORMATION; CONTROLLED STUDY; HOST CELL; LUNG FIBROSIS; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; TRANSCRIPTION REGULATION; TYPE III SECRETION SYSTEM;

EID: 84903589659     PISSN: 13500872     EISSN: 14652080     Source Type: Journal    
DOI: 10.1099/mic.0.075291-0     Document Type: Article

References (44)

1. Blier, A. S., Veron, W., Bazire, A., Gerault, E., Taupin, L., Vieillard, J., Rehel, K., Dufour, A., Le Derf, F. & other authors (2011). C-type natriuretic peptide modulates quorum sensing molecule and toxin production in Pseudomonas aeruginosa. Microbiology 157, 1929-1944.
2. Bouffartigues, E., Gicquel, G., Bazire, A., Bains, M., Maillot, O., Vieillard, J., Feuilloley, M. G., Orange, N., Hancock, R. E. & other authors (2012). Transcription of the oprF gene of Pseudomonas aeruginosa is dependent mainly on the SigX sigma factor and is sucrose induced. J Bacteriol 194, 4301-4311.
3. Boyd, A. & Chakrabarty, A. M. (1994). Role of alginate lyase in cell detachment of Pseudomonas aeruginosa. Appl Environ Microbiol 60, 2355-2359.
4. Boyer, H. W. & Roulland-Dussoix, D. (1969). A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol 41, 459-472.
5. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248-254.
6. de Lorenzo, V. & Timmis, K. N. (1994). Analysis and construction of stable phenotypes in gram-negative bacteria with Tn5-and Tn10-derived minitransposons. Methods Enzymol 235, 386-405.
7. Delic-Attree, I., Toussaint, B., Garin, J. & Vignais, P. M. (1997). Cloning, sequence and mutagenesis of the structural gene of Pseudomonas aeruginosa CysB, which can activate algD transcription. Mol Microbiol 24, 1275-1284.
8. Duan, K. & Surette, M. G. (2007). Environmental regulation of Pseudomonas aeruginosa PAO1 Las and Rhl quorum-sensing systems. J Bacteriol 189, 4827-4836.
9. Fogle, M. R., Griswold, J. A., Oliver, J. W. & Hamood, A. N. (2002). Anti-ETA IgG neutralizes the effects of Pseudomonas aeruginosa exotoxin A. J Surg Res 106, 86-98.
10. Frisk, A., Schurr, J. R., Wang, G., Bertucci, D. C., Marrero, L., Hwang, S. H., Hassett, D. J. & Schurr, M. J. (2004). Transcriptome analysis of Pseudomonas aeruginosa after interaction with human airway epithelial cells. Infect Immun 72, 5433-5438.
11. Furukawa, S., Kuchma, S. L. & O'Toole, G. A. (2006). Keeping their options open: acute versus persistent infections. J Bacteriol 188, 1211-1217.
12. Gjermansen, M., Ragas, P., Sternberg, C., Molin, S. & Tolker-Nielsen, T. (2005). Characterization of starvation-induced dispersion in Pseudomonas putida biofilms. Environ Microbiol 7, 894-906.
13. Harrison, F. (2007). Microbial ecology of the cystic fibrosis lung. Microbiology 153, 917-923.
14. Heeb, S., Itoh, Y., Nishijyo, T., Schnider, U., Keel, C., Wade, J., Walsh, U., O'Gara, F. & Haas, D. (2000). Small, stable shuttle vectors based on the minimal pVS1 replicon for use in gram-negative, plant-associated bacteria. Mol Plant Microbe Interact 13, 232-237.
15. Iglewski, B. H. & Kabat, D. (1975). NAD-dependent inhibition of protein synthesis by Pseudomonas aeruginosa toxin. Proc Natl Acad Sci U S A 72, 2284-2288.
16. Jin, Y., Yang, H., Qiao, M. & Jin, S. (2011). MexT regulates the type III secretion system through MexS and PtrC in Pseudomonas aeruginosa. J Bacteriol 193, 399-410.
17. Keen, N. T., Tamaki, S., Kobayashi, D. & Trollinger, D. (1988). Improved broad-host-range plasmids for DNA cloning in Gramnegative bacteria. Gene 70, 191-197.
18. Koch, C. & Høiby, N. (1993). Pathogenesis of cystic fibrosis. Lancet 341, 1065-1069.
19. Kuehn, M. J., Heuser, J., Normark, S. & Hultgren, S. J. (1992). P pili in uropathogenic E. coli are composite fibres with distinct fibrillar adhesive tips. Nature 356, 252-255.
20. Kulasekara, H. D., Ventre, I., Kulasekara, B. R., Lazdunski, A., Filloux, A. & Lory, S. (2005). A novel two-component system controls the expression of Pseudomonas aeruginosa fimbrial cup genes. Mol Microbiol 55, 368-380.
21. Liberati, N. T., Urbach, J. M., Miyata, S., Lee, D. G., Drenkard, E., Wu, G., Villanueva, J., Wei, T. & Ausubel, F. M. (2006). An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc Natl Acad Sci U S A 103, 2833-2838.
22. Lizewski, S. E., Schurr, J. R., Jackson, D. W., Frisk, A., Carterson, A. J. & Schurr, M. J. (2004). Identification of AlgR-regulated genes in Pseudomonas aeruginosa by use of microarray analysis. J Bacteriol 186, 5672-5684.
23. Lyczak, J. B., Cannon, C. L. & Pier, G. B. (2002). Lung infections associated with cystic fibrosis. Clin Microbiol Rev 15, 194-222.
24. Maddocks, S. E. & Oyston, P. C. (2008). Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins. Microbiology 154, 3609-3623.
25. Matsumoto, T., Furuya, N., Tateda, K., Miyazaki, S., Ohno, A., Ishii, Y., Hirakata, Y. & Yamaguchi, K. (1999). Effect of passive immunotherapy on murine gut-derived sepsis caused by Pseudomonas aeruginosa. J Med Microbiol 48, 765-770.
26. Meissner, A., Wild, V., Simm, R., Rohde, M., Erck, C., Bredenbruch, F., Morr, M., Römling, U. & Häussler, S. (2007). Pseudomonas aeruginosa cupA-encoded fimbriae expression is regulated by a GGDEF and EAL domain-dependent modulation of the intracellular level of cyclic diguanylate. Environ Microbiol 9, 2475-2485.
27. Miller, J. H. (1972). Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
28. Mulcahy, H. & Lewenza, S. (2011). Magnesium limitation is an environmental trigger of the Pseudomonas aeruginosa biofilm lifestyle. PLoS ONE 6, e23307.
29. Mulcahy, H., O'Callaghan, J., O'Grady, E. P., Adams, C. & O'Gara, F. (2006). The posttranscriptional regulator RsmA plays a role in the interaction between Pseudomonas aeruginosa and human airway epithelial cells by positively regulating the type III secretion system. Infect Immun 74, 3012-3015.
30. O'Toole, G. A. & Kolter, R. (1998). The initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signaling pathways: a genetic analysis. Mol Microbiol 28, 449-461.
31. Parsek, M. R., Ye, R. W., Pun, P. & Chakrabarty, A. M. (1994). Critical nucleotides in the interaction of a LysR-type regulator with its target promoter region. catBC promoter activation by CatR. J Biol Chem 269, 11279-11284.
32. Reen, F. J., Barret, M., Fargier, E., O'Muinneacháin, M. & O'Gara, F. (2013a). Molecular evolution of LysR-type transcriptional regulation in Pseudomonas aeruginosa. Mol Phylogenet Evol 66, 1041-1049.
33. Reen, F. J., Haynes, J. M., Mooij, M. J. & O'Gara, F. (2013b). A nonclassical LysR-type transcriptional regulator PA2206 is required for an effective oxidative stress response in Pseudomonas aeruginosa. PLoS ONE 8, e54479.
34. Sambrook, J. & Russell, D. W. (2001). Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
35. Schell, M. A. (1993). Molecular biology of the LysR family of transcriptional regulators. Annu Rev Microbiol 47, 597-626.
36. Spaink, H. P., Okker, R. J. H., Wijffelman, C. A., Pees, E. & Lugtenberg, B. J. J. (1987). Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI. Plant Mol Biol 9, 27-39.
37. Sriramulu, D. D., Lünsdorf, H., Lam, J. S. & Römling, U. (2005). Microcolony formation: a novel biofilm model of Pseudomonas aeruginosa for the cystic fibrosis lung. J Med Microbiol 54, 667-676.
38. Sulston, J. & Hodgkin, J. (1988). The Nematode Caenorhabditis elegans. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
39. Tian, Z. X., Fargier, E., Mac Aogáin, M., Adams, C., Wang, Y. P. & O'Gara, F. (2009). Transcriptome profiling defines a novel regulon modulated by the LysR-type transcriptional regulator MexT in Pseudomonas aeruginosa. Nucleic Acids Res 37, 7546-7559.
40. Vallet, I., Olson, J. W., Lory, S., Lazdunski, A. & Filloux, A. (2001). The chaperone/usher pathways of Pseudomonas aeruginosa: identification of fimbrial gene clusters (cup) and their involvement in biofilm formation. Proc Natl Acad Sci U S A 98, 6911-6916.
41. Wade, D. S., Calfee, M. W., Rocha, E. R., Ling, E. A., Engstrom, E., Coleman, J. P. & Pesci, E. C. (2005). Regulation of Pseudomonas quinolone signal synthesis in Pseudomonas aeruginosa. J Bacteriol 187, 4372-4380.
42. Worlitzsch, D., Tarran, R., Ulrich, M., Schwab, U., Cekici, A., Meyer, K. C., Birrer, P., Bellon, G., Berger, J. & other authors (2002). Effects of reduced mucus oxygen concentration in airway Pseudomonas infections of cystic fibrosis patients. J Clin Invest 109, 317-325.
43. Yahr, T. L. & Wolfgang, M. C. (2006). Transcriptional regulation of the Pseudomonas aeruginosa type III secretion system. Mol Microbiol 62, 631-640.
44. Yahr, T. L., Hovey, A. K., Kulich, S. M. & Frank, D. W. (1995). Transcriptional analysis of the Pseudomonas aeruginosa exoenzyme S structural gene. J Bacteriol 177, 1169-1178.