The fractured genome of HeLa cells

Genome Biology

Volumn 14, Issue 4, 2013, Pages

  Mittelman, David ^a,b   Wilson, John H ^c  

^a VIRGINIA BIOINFORMATICS INSTITUTE   (United States)

^b VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

^c BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Cell line identification; Genome instability; HeLa cells; High throughput sequencing; Tandem repeats

Indexed keywords

DNA; RNA; SMALL INTERFERING RNA;

ARTICLE; CELL IMMORTALIZATION; CELL PROLIFERATION; CHROMOSOME 11; CHROMOSOME ABERRATION; COPY NUMBER VARIATION; CYTOGENETICS; DNA SEQUENCE; FLUORESCENCE IN SITU HYBRIDIZATION; GENE EXPRESSION; GENETIC HETEROGENEITY; GENETIC VARIABILITY; GENOMICS; HELA CELL LINE; HIGH THROUGHPUT SEQUENCING; HOMOZYGOSITY; HUMAN; MEDICAL RESEARCH; RNA SEQUENCE; SHORT TANDEM REPEAT; UTERINE CERVIX CANCER; HUMAN GENOME;

GENOME, HUMAN; HUMANS;

EID: 84903145295     PISSN: 14747596     EISSN: 1474760X     Source Type: Journal    
DOI: 10.1186/gb-2013-14-4-111     Document Type: Article

References (8)

1. Morales CP, Holt SE, Ouellette M, Kaur KJ, Yan Y, Wilson KS, White MA, Wright WE, Shay JW: Absence of cancer-associated changes in human fibroblasts immortalized with telomerase. Nat Genet 1999, 21:115-118.
2. Landry J, Pyl PT, Rausch T, Zichner T, Tekkedil MM, Stütz AM, Jauch A, Aiyar RS, Pau G, Delhomme N, Gagneur J, Korbel JO, Huber W, Steinmetz LM: The genomic and transcriptomic landscape of a HeLa cell line. G3 2013, doi:10.1534/g3.113.005777.
3. Macville M, Schrock E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T: Comprehensive and definitive molecular cytogeneticcharacterization of HeLa cells by spectral karyotyping. Cancer Res 1999, 59:141-150.
4. Stephens PJ, Greenman CD, Fu B, Yang F, Bignell GR, Mudie LJ, Pleasance ED, Lau KW, Beare D, Stebbings LA, McLaren S, Lin ML, McBride DJ, Varela I, Nik- Zainal S, Leroy C, Jia M, Menzies A, Butler AP, Teague JW, Quail MA, Burton J, Swerdlow H, Carter NP, Morsberger LA, Iacobuzio-Donahue C, Follows GA, Green AR, Flanagan AM, Stratton MR, et al.: Massive genomic rearrangement acquired in a single catastrophic event during cancer development. Cell 2011, 144:27-40.
5. Alkan C, Coe BP, Eichler EE: Genome structural variation discovery and genotyping. Nat Rev Genet 2011, 12:363-376.
6. Rausch T, Zichner T, Schlattl A, Stutz AM, Benes V, Korbel JO: DELLY: structural variant discovery by integrated paired-end and split-read analysis. Bioinformatics 2012, 28:i333-i339.
7. Masters JR: Cell-line authentication: end the scandal of false cell lines. Nature 2012, 492:186.
8. Highnam G, Franck C, Martin A, Stephens C, Puthige A, Mittelman D: Accurate human microsatellite genotypes from high-throughput resequencing data using informed error profiles. Nucleic Acids Res 2013, 41:e32.