Elution of High-affinity (>10-9 KD) Antibodies from Tissue Sections: Clues to the Molecular Mechanism and Use in Sequential Immunostaining

Journal of Histochemistry and Cytochemistry

Volumn 62, Issue 7, 2014, Pages 519-531

  Gendusa, Rossella ^a   Scalia, Carla Rossana ^a   Buscone, Serena ^b   Cattoretti, Giorgio ^a,b  

^a SAN GERARDO HOSPITAL   (Italy)

^b UNIVERSITY OF MILANO BICOCCA   (Italy)

Author keywords

affinity; antibody; elution; multiple immunostaining; stripping

Indexed keywords

ANTIBODY; BUFFER; GLYCINE; IMMUNOGLOBULIN F(AB) FRAGMENT; MERCAPTOETHANOL; STREPTAVIDIN; UREA; DODECYL SULFATE SODIUM; FIXATIVE; FORMALDEHYDE; MONOCLONAL ANTIBODY; PARAFFIN;

ANTIBODY AFFINITY; ANTIGEN RETRIEVAL; ARTICLE; CONTROLLED STUDY; CROSS LINKING; ELUTION; HUMAN; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; MOLECULAR MECHANICS; PH; PRIORITY JOURNAL; TISSUE REACTION; ANIMAL; CHEMISTRY; GASTROINTESTINAL TRACT; ISOLATION AND PURIFICATION; KIDNEY; PALATINE TONSIL; PROCEDURES; SKIN; STAINING;

ANIMALS; ANTIBODIES, MONOCLONAL; BUFFERS; FIXATIVES; FORMALDEHYDE; GASTROINTESTINAL TRACT; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOHISTOCHEMISTRY; KIDNEY; MERCAPTOETHANOL; PALATINE TONSIL; PARAFFIN EMBEDDING; SKIN; SODIUM DODECYL SULFATE; STAINING AND LABELING;

EID: 84903142018     PISSN: 00221554     EISSN: 15515044     Source Type: Journal    
DOI: 10.1369/0022155414536732     Document Type: Article

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