Lactobacillus crispatus L1: High cell density cultivation and exopolysaccharide structure characterization to highlight potentially beneficial effects against vaginal pathogens

BMC Microbiology

Volumn 14, Issue 1, 2014, Pages

  Donnarumma, Giovanna ^a   Molinaro, Antonio ^b   Cimini, Donatella ^a   De Castro, Cristina ^b   Valli, Vivien ^a   De Gregorio, Vincenza ^a   De Rosa, Mario ^a   Schiraldi, Chiara ^a  

^a SECOND UNIVERSITY OF NAPLES   (Italy)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

High cell densities cultivation; L. crispatus L1; Simulated digestion

Indexed keywords

BETA DEFENSIN 2; CARBON; EXOPOLYSACCHARIDE; GLUCOSE; LACTIC ACID; PROBIOTIC AGENT; BACTERIAL POLYSACCHARIDE; CARBOXYLIC ACID;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CANDIDA ALBICANS; CARBON SOURCE; CELL ADHESION; CELL DENSITY; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; DIGESTION; FEMALE; FERMENTATION; GROWTH INHIBITION; HUMAN; HUMAN CELL; IN VITRO STUDY; LACTOBACILLUS; LACTOBACILLUS CRISPATUS; MICROBIAL BIOMASS; MICROFILTRATION; MOLECULAR STABILITY; NONHUMAN; PROTEIN EXPRESSION; PROTON NUCLEAR MAGNETIC RESONANCE; PURIFICATION; REDUCTION; SIMULATION; STRUCTURE ANALYSIS; VAGINA FLORA; VAGINAL SECRETION; ANTIBIOSIS; CELL COUNT; CHEMISTRY; DRUG EFFECTS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM; MICROBIAL VIABILITY; MICROBIOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; ORGANISMAL INTERACTION; PANCREAS JUICE; PHYSIOLOGY; VAGINA;

CANDIDA ALBICANS; LACTOBACILLUS CRISPATUS;

ANTIBIOSIS; CANDIDA ALBICANS; CARBOXYLIC ACIDS; CELL ADHESION; CELL COUNT; FEMALE; GLUCOSE; HUMANS; LACTOBACILLUS; MAGNETIC RESONANCE SPECTROSCOPY; MICROBIAL INTERACTIONS; MICROBIAL VIABILITY; PANCREATIC JUICE; POLYSACCHARIDES, BACTERIAL; PROBIOTICS; VAGINA;

EID: 84902545049     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-14-137     Document Type: Article

References (47)

1. Interactions between the microbiota and the intestinal mucosa. Schiffrin EJ, Blum S, Eur J Clin Nutr 2002 56 Suppl 3 60 S64 12142966
2. Beneficial effects of administration of Lactobacillus acidophilus in diarrheal and other intestinal disorders. Beck CNH, Am J Gastroenterol 1961 35 522 530 13688370
3. Ingestion of yogurt containing Lactobacillus acidophilus as prophylaxis for candidal vaginitis. Hilton E, Isenberg HD, Alperstein P, France K, Borenstein MT, Ann Intern Med 1992 116 353 357 10.7326/0003-4819-116-5-353 1736766
4. In vitro probiotic properties of Lactobacillus fermentum SK5 isolated from vagina of a healthy woman. Kaewnopparat S, Dangmanee N, Kaewnopparat N, Srichana T, Chulasiri M, Settharaksa S, Anaerobe 2013 22 6 13 23624069
5. Bacterial vaginosis: a review on clinical trials with probiotics. Mastromarino P, Vitali B, Mosca L, New Microbiol 2013 36 229 238 23912864
6. Urogenital Lactobacilli Probiotics, Reliability, and Regulatory Issues. Reid GZCGG, J Dairy Sci 2001 84 164 E169
7. A human Lactobacillus strain (Lactobacillus casei sp strain GG) promotes recovery from acute diarrhea in children. Isolauri E, Juntunen M, Rautanen T, Sillanaukee P, Koivula T, Pediatrics 1991 88 90 97 1905394
8. Lactobacillus by-products inhibit the growth and virulence of uropathogenic Escherichia coli. Cadieux PA, Burton J, Devillard E, Reid G, J Physiol Pharmacol 2009 60 Suppl 6 13 18
9. Clinical study comparing probiotic Lactobacillus GR-1 and RC-14 with metronidazole vaginal gel to treat symptomatic bacterial vaginosis. Anukam KC, Osazuwa E, Osemene GI, Ehigiagbe F, Bruce AW, Reid G, Microbes Infect 2006 8 2772 2776 10.1016/j.micinf.2006.08.008 17045832
10. The role of vaginal Lactobacillus Rhamnosus (Normogin(R)) in preventing Bacterial Vaginosis in women with history of recurrences, undergoing surgical menopause: a prospective pilot study. Parma M, Dindelli M, Caputo L, Redaelli A, Quaranta L, Candiani M, Eur Rev Med Pharmacol Sci 2013 17 1399 1403 23740456
11. Acid production by vaginal flora in vitro is consistent with the rate and extent of vaginal acidification. Boskey ER, Telsch KM, Whaley KJ, Moench TR, Cone RA, Infect Immun 1999 67 5170 5175 10496892
12. Factors associated with acquisition of, or persistent colonization by, vaginal lactobacilli: role of hydrogen peroxide production. Vallor AC, Antonio MA, Hawes SE, Hillier SL, J Infect Dis 2001 184 1431 1436 10.1086/324445 11709785
13. Effects of probiotics on the recurrence of bacterial vaginosis: a review. Homayouni A, Bastani P, Ziyadi S, Mohammad-Alizadeh-Charandabi S, Ghalibaf M, Mortazavian AM, Mehrabany EV, J Low Genit Tract Dis 2014 18 79 86 10.1097/LGT.0b013e31829156ec 24299970
14. Intravaginal instillation of lactobacilli for prevention of recurrent urinary tract infections. Bruce AW, Reid G, Can J Microbiol 1988 34 339 343 10.1139/m88-062 3138016
15. Prevalence of hydrogen peroxide-producing Lactobacillus species in normal women and women with bacterial vaginosis. Eschenbach DA, Davick PR, Williams BL, Klebanoff SJ, Young-Smith K, Critchlow CM, Holmes KK, J Clin Microbiol 1989 27 251 256 2915019
16. Oral probiotics can resolve urogenital infections. Reid G, Bruce AW, Fraser N, Heinemann C, Owen J, Henning B, FEMS Immunol Med Microbiol 2001 30 49 52 10.1111/j.1574-695X.2001.tb01549.x 11172991
17. Utilization of the intestinal tract as a delivery system for urogenital probiotics. Morelli L, Zonenenschain D, Del PM, Cognein P, J Clin Gastroenterol 2004 38 107 S110 10.1097/01.mcg.0000128938.32835.98 15220672
18. Ecological role of lactobacilli in the gastrointestinal tract: implications for fundamental and biomedical research. Walter J, Appl Environ Microbiol 2008 74 4985 4996 10.1128/AEM.00753-08 18539818
19. Vaginal microbiome: rethinking health and disease. Ma B, Forney LJ, Ravel J, Annu Rev Microbiol 2012 66 371 389 10.1146/annurev-micro-092611-150157 22746335
20. Screening for lactobacilli with probiotic properties in the infant gut microbiota. Kirtzalidou E, Pramateftaki P, Kotsou M, Kyriacou A, Anaerobe 2011 17 440 443 10.1016/j.anaerobe.2011.05.007 21621627
21. Lactobacillus rhamnosus L60, a potential probiotic isolated from the human vagina. Pascual LM, Daniele MB, Ruiz F, Giordano W, Pajaro C, Barberis L, J Gen Appl Microbiol 2008 54 141 148 10.2323/jgam.54.141 18654035
22. Adherence of clinically isolated lactobacilli to human cervical cells in competition with Neisseria gonorrhoeae. Vielfort K, Sjolinder H, Roos S, Jonsson H, Aro H, Microbes Infect 2008 10 1325 1334 10.1016/j.micinf.2008.07.032 18761100
23. Bactericidal activity of culture fluid components of Lactobacillus fermentum strain 90 TS-4 (21) clone 3, and their capacity to modulate adhesion of Candida albicans yeast-like fungi to vaginal epithelial cells. Anokhina IV, Kravtsov EG, Protsenko AV, Yashina NV, Yermolaev AV, Chesnokova VL, Dalin MV, Bull Exp Biol Med 2007 143 359 362 10.1007/s10517-007-0112-0 18225764
24. Mammalian defensins in the antimicrobial immune response. Selsted ME, Ouellette AJ, Nat Immunol 2005 6 551 557 10.1038/ni1206 15908936
25. Basic local alignment search tool. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ, J Mol Biol 1990 215 403 410 10.1016/S0022-2836(05)80360-2 2231712
26. Identification, structure, and characterization of an exopolysaccharide produced by Histophilus somni during biofilm formation. Sandal I, Inzana TJ, Molinaro A, De CC, Shao JQ, Apicella MA, Cox AD, St MF, Berg G, BMC Microbiol 2011 11 186 10.1186/1471-2180-11-186 21854629
27. Importance of Candida-bacterial polymicrobial biofilms in disease. Harriott MM, Noverr MC, Trends Microbiol 2011 19 557 563 10.1016/j.tim.2011.07. 004 21855346
28. Vaginal lactobacillus flora of healthy Swedish women. Vasquez A, Jakobsson T, Ahrne S, Forsum U, Molin G, J Clin Microbiol 2002 40 2746 2749 10.1128/JCM.40.8.2746-2749.2002 12149323
29. Multiplex quantitative polymerase chain reaction assay for the identification and quantitation of major vaginal lactobacilli. Balashov SV, Mordechai E, Adelson ME, Sobel JD, Gygax SE, Diagn Microbiol Infect Dis 2014 78 321 327 10.1016/j.diagmicrobio.2013.08.004 24445159
30. Lactobacillus-dominated cervicovaginal microbiota associated with reduced HIV/STI prevalence and genital HIV viral load in African women. Borgdorff H, Tsivtsivadze E, Verhelst R, Marzorati M, Jurriaans S, Ndayisaba GF, Schuren FH, van de Wijgert JH, ISME J 2014 2014 2014
31. [Vaginal microbiota: composition, protective role, associated pathologies, and therapeutic perspectives]. Martin R, Soberon N, Vazquez F, Suarez JE, Enferm Infecc Microbiol Clin 2008 26 160 167 10.1157/13116753 18358215
32. Kinetic study of the conversion of different substrates to lactic acid using Lactobacillus bulgaricus. Burgos-Rubio CN, Okos MR, Wankat PC, Biotechnol Prog 2000 16 305 314 10.1021/bp000022p 10835228
33. Augmentation of antimicrobial metronidazole therapy of bacterial vaginosis with oral probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14: randomized, double-blind, placebo controlled trial. Anukam K, Osazuwa E, Ahonkhai I, Ngwu M, Osemene G, Bruce AW, Reid G, Microbes Infect 2006 8 1450 1454 10.1016/j.micinf.2006.01.003 16697231
34. High cell density cultivation of probiotics and lactic acid production. Schiraldi C, Adduci V, Valli V, Maresca C, Giuliano M, Lamberti M, Carteni M, De RM, Biotechnol Bioeng 2003 82 213 222 10.1002/bit.10557 12584763
35. Bifidobacterial surface-exopolysaccharide facilitates commensal-host interaction through immune modulation and pathogen protection. Fanning S, Hall LJ, Cronin M, Zomer A, MacSharry J, Goulding D, Motherway MO, Shanahan F, Nally K, Dougan G, van Sinderen D, Proc Natl Acad Sci U S A 2012 109 2108 2113 10.1073/pnas.1115621109 22308390
36. Identification of genes associated with the long-gut-persistence phenotype of the probiotic Lactobacillus johnsonii strain NCC533 using a combination of genomics and transcriptome analysis. Denou E, Pridmore RD, Berger B, Panoff JM, Arigoni F, Brussow H, J Bacteriol 2008 190 3161 3168 10.1128/JB.01637-07 18223069
37. Candida albicans primes TLR cytokine responses through a Dectin-1/Raf-1-mediated pathway. Ifrim DC, Joosten LA, Kullberg BJ, Jacobs L, Jansen T, Williams DL, Gow NA, van der Meer JW, Netea MG, Quintin J, J Immunol 2013 190 4129 4135 10.4049/jimmunol.1202611 23475217
38. Development of a growth medium suitable for exopolysaccharide production by Lactobacillus delbrueckii ssp. bulgaricus RR. Kimmel SA, Roberts RF, Int J Food Microbiol 1998 40 87 92 10.1016/S0168-1605(98)00023-3 9600614
39. Urogenital pathogen inhibition and compatibility between vaginal Lactobacillus strains to be considered as probiotic candidates. Juarez Tomas MS, Saralegui Duhart CI, De Gregorio PR, Vera PE, Nader-Macias ME, Eur J Obstet Gynecol Reprod Biol 2011 159 399 406 10.1016/j.ejogrb.2011.07.010 21862199
40. Compilation of small ribosomal subunit RNA structures. Neefs JM, Van de Peer Y, De RP, Chapelle S, De WR, Nucleic Acids Res 1993 21 3025 3049 10.1093/nar/21.13.3025 8332525
41. Production of antimicrobial substances by lactic acid bacteria I: determination of hydrogen peroxide. Tomas MS, Claudia OM, Ocana V, Elena Nader-Macias M, Methods Mol Biol 2004 268 337 346 15156044
42. Effect of Protectors on the Viability of Lactobacillus acidophilus M92 in Simulated Gastrointestinal Conditions. Kos BSJGJMS, Food technol Biotechnol 2000 38 121 127
43. Improvement in anthrone method for the determination of carbohydrates. Loweus FA, Anal Chem 1952 24 19
44. The O-specific polysaccharide structure and gene cluster of serotype O:12 of the Yersinia pseudotuberculosis complex, and the identification of a novel L-quinovose biosynthesis gene. De Castro C, Kenyon JJ, Cunneen MM, Molinaro A, Holst O, Skurnik M, Reeves PR, Glycobiology 2013 23 346 353 10.1093/glycob/ cws145 23077132
45. Microbe-associated molecular patterns in innate immunity: Extraction and chemical analysis of gram-negative bacterial lipopolysaccharides. De Castro C, Parrilli M, Holst O, Molinaro A, Methods Enzymol 2010 480 89 115 20816206
46. Technological and biological evaluation of tablets containing different strains of lactobacilli for vaginal administration. Maggi L, Mastromarino P, Macchia S, Brigidi P, Pirovano F, Matteuzzi D, Conte U, Eur J Pharm Biopharm 2000 50 389 395 10.1016/S0939-6411(00)00121-1 11072196
47. Assessment of the capacity of Lactobacillus to inhibit the growth of uropathogens and block their adhesion to vaginal epithelial cells. Osset J, Bartolome RM, Garcia E, Andreu A, J Infect Dis 2001 183 485 491 10.1086/318070 11133381