Comparison of procedures for the extraction of supernatants and cytotoxicity tests in Vero cells, applied to assess the toxigenic potential of Bacillus spp. and Lactobacillus spp., intended for use as probiotic strains

Journal of Microbiological Methods

Volumn 103, Issue , 2014, Pages 64-69

  Blanch, Anicet R ^a   Méndez, Javier ^a   Castel, Susana ^a   Reina, Manuel ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

Author keywords

Ammonium sulphate; Bacillus; Extraction of supernatants; Microbial feed additive; Ultrafiltration; Vero cells

Indexed keywords

AMMONIUM SULFATE; LACTATE DEHYDROGENASE; PROBIOTIC AGENT; TETRAZOLIUM; UNCLASSIFIED DRUG; WATER SOLUBLE TETRAZOLIUM 1; CULTURE MEDIUM;

ARTICLE; BACILLUS; BACILLUS CEREUS; BACILLUS SUBTILIS; BACILLUS THURINGIENSIS; BACILLUS TOYONENSIS; BACTERIAL STRAIN; CELL ASSAY; CELL SURVIVAL; CONCENTRATION PROCESS; CONTROLLED STUDY; CYTOTOXICITY TEST; ENTEROTOXICITY; ENZYME RELEASE; EXTRACTION; FEASIBILITY STUDY; INTERMETHOD COMPARISON; LACTOBACILLUS; LACTOBACILLUS REUTERI; LACTOBACILLUS RHAMNOSUS; METHANOL EXTRACTION; PRIORITY JOURNAL; PROTEIN SYNTHESIS INHIBITION; SENSITIVITY ANALYSIS; SUPERNATANT; SURVIVAL RATE; TOXICITY; ULTRAFILTRATION; VERO CELL LINE; ANIMAL; CELL CULTURE; CHLOROCEBUS AETHIOPS; CULTURE MEDIUM; METABOLISM; PROCEDURES; TOXICITY TESTING;

ANIMALS; BACILLUS; CELLS, CULTURED; CERCOPITHECUS AETHIOPS; CULTURE MEDIA, CONDITIONED; LACTOBACILLUS; PROBIOTICS; TOXICITY TESTS; VERO CELLS;

EID: 84902336627     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2014.05.019     Document Type: Article

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