The use of a porcine intestinal cell model system for evaluating the food safety risk of Bacillus cereus probiotics and the implications for assessing enterotoxigenicity

APMIS

Volumn 119, Issue 12, 2011, Pages 877-884

  Trapecar, Martin ^a   Leouffre, Thomas ^a   Faure, Morgane ^a   Jensen, Henrik E ^b   Granum, Per E ^c   Cencic, Avrelija ^a   Hardy, Simon P ^c,d  

^a UNIVERSITY OF MARIBOR   (Slovenia)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

^c NORWEGIAN UNIVERSITY OF LIFE SCIENCES   (Norway)

^d NORWEGIAN VETERINARY INSTITUTE   (Norway)

Author keywords

Animal models; Cytotoxicity; Enterotoxins; Functional cell models; Pig; Risk assessment

Indexed keywords

NITRIC OXIDE; PROBIOTIC AGENT;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BACILLUS CEREUS; BACTERIAL STRAIN; BINDING AFFINITY; CYTOTOXICITY; DIARRHEA; DILUTION; ENTEROTOXICITY; FOOD SAFETY; HUMAN; HUMAN CELL; HUMAN TISSUE; INTESTINE CELL; INTESTINE INJURY; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RISK ASSESSMENT; STRAIN DIFFERENCE; SWINE; TOXICITY; WEANING;

ANIMALS; BACILLUS CEREUS; BACTERIAL ADHESION; CELL LINE; FOOD SAFETY; INTESTINAL MUCOSA; NITRIC OXIDE; PROBIOTICS; RISK ASSESSMENT; SWINE;

EID: 81355160255     PISSN: 09034641     EISSN: 16000463     Source Type: Journal    
DOI: 10.1111/j.1600-0463.2011.02797.x     Document Type: Article

References (20)

1. Stenfors Arnesen LP, Fagerlund A, Granum PE. From soil to gut: Bacillus cereus and its food poisoning toxins. FEMS Microbiol Rev 2008;32:579-606.
2. Hong HA, Duc le H, Cutting SM. The use of bacterial spore formers as probiotics. FEMS Microbiol Rev 2005;29:813-35.
3. EFSA. Opinion of the scientific panel on biological hazards on Bacillus cereus and other Bacillus spp in foodstuff. EFSA J 2005;175:1-48.
4. EFSA. Opinion of the Scientific Committee on a request from EFSA on the introduction of a Qualified Presumption of Safety (QPS) approach for assessment of selected microorganisms referred to EFSA. EFSA J 2007;587:1-16.
5. Wehrle E, Moravek M, Dietrich R, Burk C, Didier A, Märtlbauer E. Comparison of multiplex PCR, enzyme immunoassay and cell culture methods for the detection of enterotoxinogenic Bacillus cereus. J Microbiol Meth 2009;78:265-70.
6. Beecher DJ, Schoeni JL, Wong AC. Enterotoxic activity of hemolysin BL from Bacillus cereus. Infect Immun 1995;63:4423-8.
7. Lund T, Granum PE. Characterisation of a non-haemolytic enterotoxin complex from Bacillus cereus isolated after a foodborne outbreak. FEMS Microbiol Lett 1996;141:151-6.
8. Haug TM, Sand SL, Sand O, Phung D, Granum PE, Hardy SP. Formation of very large conductance channels by Bacillus cereus Nhe in Vero and GH(4) cells identifies NheA + B as the inherent pore-forming structure. J Membr Biol 2010;237:1-11.
9. Cencic A, Langerholc T. Functional cell models of the gut and their applications in food microbiology - A review. Int J Food Microbiol 2010;141(Suppl 1):S4-14.
10. Lindbäck T, Okstad OA, Rishovd AL, Kolstø AB. Insertional inactivation of hblC encoding the L2 component of Bacillus cereus ATCC 14579 haemolysin BL strongly reduces enterotoxigenic activity, but not the haemolytic activity against human erythrocytes. Microbiology 1999;145:3139-46.
11. Ivec M, Botic T, Koren S, Jakobsen M, Weingartl H, Cencic A. Interactions of macrophages with probiotic bacteria lead to increased antiviral response against vesicular stomatitis virus. Antiviral Res 2007;75:266-74.
12. Pipenbaher N, Moeller PL, Dolinsek J, Jakobsen M, Weingartl H, Cencic A. Nitric oxide (NO) production in mammalian non-tumorigenic epithelial cells of the small intestine and macrophages induced by individual strains of lactobacilli and bifidobacteria. Int Dairy J 2009;19:166-71.
13. Dietrich R, Moravek M, Burk C, Granum PE, Martlbauer E. Production and characterization of antibodies against each of the three subunits of the Bacillus cereus non-hemolytic enterotoxin complex. Appl Environ Microbiol 2005;71:8214-20.
14. Duc le H, Hong HA, Barbosa TM, Henriques AO, Cutting SM. Characterization of Bacillus probiotics available for human use. Appl Environ Microbiol 2004;70:2161-71.
15. Williams L, Burdock G, Jimenez G, Castillo M. Literature review on the safety of Toyocerin™, a non-toxigenic and non-pathogenic Bacillus cereus var. toyoi preparation. Reg Toxicol Pharmacol 2009;55:236-24.
16. Ramarao N, Lereclus D. Adhesion and cytotoxicity of Bacillus cereus and Bacillus thuringiensis to epithelial cells are FlhA and PlcR dependent, respectively. Microbes Infect 2006;8:1483-91.
17. Guilloteau P, Zabielski R, Hammon HM, Metges CC. Nutritional programming of gastrointestinal tract development. Is the pig a good model for man?. Nutr Res Rev 2010;23:4-22.
18. Patterson JK, Lei XG, Miller DD. The pig as an experimental model for elucidating the mechanisms governing dietary influence on mineral absorption. Exp Biol Med 2008;233:651-64.
19. Ehling-Schulz M, Svensson B, Guinebretiere MH, Lindbäck T, Andersson M, Schulz A, et al. Emetic toxin formation of Bacillus cereus is restricted to a single evolutionary lineage of closely related strains. Microbiology 2005;151:183-97.
20. Fagerlund A, Lindbäck T, Storset AK, Granum PE, Hardy SP. Bacillus cereus Nhe is a pore-forming toxin with structural and functional properties similar to the ClyA (HlyE, SheA) family of haemolysins, able to induce osmotic lysis in epithelia. Microbiology 2008;154:693-704.