Epigallocatechin-3-gallate inhibits proliferation of human aortic smooth muscle cells via up-regulating expression of mitofusin 2

European Journal of Cell Biology

Volumn 93, Issue 4, 2014, Pages 137-144

  Shu, Zhouwu ^a   Yu, Min ^a   Zeng, Guoning ^a   Zhang, Xin ^a   Wu, Libiao ^a   Tan, Xuerui ^a  

^a THE FIRST AFFILIATED HOSPITAL OF SHANTOU UNIVERSITY MEDICAL COLLEGE   (China)

Author keywords

Epigallocatechin 3 gallate; Human aortic smooth muscle cell; Mitofusin 2; Proliferation; Ras Raf ERK MAPK; SiRNA

Indexed keywords

EDOXUDINE; EPIGALLOCATECHIN GALLATE; MITOFUSIN 2; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; RAF PROTEIN; RAS PROTEIN; SMALL INTERFERING RNA; CATECHIN; GUANOSINE TRIPHOSPHATASE; MAPK1 PROTEIN, HUMAN; MFN2 PROTEIN, HUMAN; MITOCHONDRIAL PROTEIN;

AORTA MEDIA; ARTICLE; CELL CULTURE; CELL PROLIFERATION; DOWN REGULATION; GENE EXPRESSION; GENE SILENCING; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE; SMOOTH MUSCLE FIBER; UPREGULATION; WESTERN BLOTTING; ANALOGS AND DERIVATIVES; AORTA; CELL LINE; CYTOLOGY; DRUG EFFECTS; GENETICS; METABOLISM;

AORTA; CATECHIN; CELL LINE; CELL PROLIFERATION; GTP PHOSPHOHYDROLASES; HUMANS; MITOCHONDRIAL PROTEINS; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; MYOCYTES, SMOOTH MUSCLE; PROTO-ONCOGENE PROTEINS C-RAF; RNA, SMALL INTERFERING; UP-REGULATION;

EID: 84902332762     PISSN: 01719335     EISSN: 16181298     Source Type: Journal    
DOI: 10.1016/j.ejcb.2014.04.001     Document Type: Article

References (32)

1. Andrés V., Castro C. Antiproliferative strategies for the treatment of vascular proliferative disease. Curr. Vasc. Pharmacol. 2003, 1:85-98.
2. Bach D., Pich S., Soriano F.X., Vega N., Baumgartner B., Oriola J., et al. Mitofusin-2 determines mitochondrial network architecture and mitochondrial metabolism. A novel regulatory mechanism altered in obesity. J. Biol. Chem. 2003, 278:17190-17197.
3. Benelli R., Vene R., Bisacchi D., Garbisa S., Albini A. Anti-invasive effects of green tea polyphenol epigallocatechin-3-gallate (EGCG), a natural inhibitor of metallo and serine proteases. Biol. Chem. 2002, 383:101-105.
4. Braun-Dullaeus R.C., Mann M.J., Dzau V.J. Cell cycle progression: new therapeutic target for vascular proliferative disease. Circulation 1998, 98:82-89.
5. Charron T., Nili N., Strauss B.H. The cell cycle: a critical therapeutic target to prevent vascular proliferative disease. Can. J. Cardiol. 2006, 22(Suppl. B):41B-55B.
6. Chen H., Detmer S.A., Ewald A.J., Griffin E.E., Fraser S.E., Chan D.C. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 2003, 160:189-200.
7. Chen J., Han Y., Lin C., Zhen Y., Song X., Teng S., et al. PDGF-D contributes to neointimal hyperplasia in rat model of vessel injury. Biochem. Biophys. Res. Commun. 2005, 329:976-983.
8. Chen K.H., Guo X., Ma D., Guo Y., Li Q., Yang D., et al. Dysregulation of HSG triggers vascular proliferation disorder. Nat. Cell Biol. 2004, 6:872-883.
9. Chen K.H., Wang F., Zhang J., Xiang H., Dong G., Zhang C., et al. Cloning and expression of a novel partial cDNA related to hypertension. Chin. Med. J. (Engl.) 1998, 111:383-384.
10. Cheng X.W., Kuzuya M., Nakamura K., Liu Z., Di Q., Hasegawa J., et al. Mechanisms of the inhibitory effect of epigallocatechin-3-gallate on cultured human vascular smooth muscle cell invasion. Arterioscler. Thromb. Vasc. Biol. 2005, 25:1864-1870.
11. Chien K.R., Hoshijima M. Unravelling Ras signals in cardiovascular disease. Nat. Cell Biol. 2004, 6:807-808.
12. Guo X.M., Chen K.H., Guo Y.H., Liao H., Tang J., Xiao R.P. Mitofusin2 triggers vascular smooth musele cell apoptosis via mitochondrial death pathway. Circ. Res. 2007, 101:1113-1122.
13. Han D.W., Jung D.Y., Park J.C., Cho H.H., Hyon S.H., Han D.K. Underlying mechanism for suppression of vascular smooth muscle cells by green tea polyphenol EGCG released from biodegradable polymers for stent application. J. Biomed. Mater. Res. A 2010, 95:424-433.
14. Hofmann C.S., Sonenshein G.E. Green tea polyphenol epigallocatechin-3 gallate induces apoptosis of proliferating vascular smooth muscle cells via activation of p53. FASEB J. 2003, 17:702-704.
15. Hwang K.C., Lee K.H., Jang Y., Yun Y.P., Chung K.H. Epigallocatechin-3-gallate inhibits basic fibroblas t growth factor-induced intracellular signaling transduction pathway in rat aortic smooth muscle cells. J. Cardiovasc. Pharmacol. 2002, 39:271-277.
16. Jin Y.R., Im J.H., Park E.S., Cho M.R., Han X.H., Lee J.J., et al. Antiplatelet activity of epigallocatechin gallate is mediated by the inhibition of PLCgamma2 phosphorylation, elevation of PGD2 production, and maintaining calcium-ATPase activity. J. Cardiovasc. Pharmacol. 2008, 51:45-54.
17. Keenan S.M., Bellone C., Baldassare J.J. Cyclin-dependent kinase 2 nucleocytoplasmic translocation is regulated by extracellular regulated kinase. J. Biol. Chem. 2001, 276:22404-22409.
18. Kyaw M., Yoshizumi M., Tsuchiya K., Kirima K., Tamaki T. Antioxidants inhibit JNK and p38 MAPK activation but not ERK 1/2 activation by angiotensin II in rat aortic smooth muscle cells. Hypertens Res. 2001, 24:251-261.
19. Liao H., Gong J., Zhang W., Guo X. Valsartan inhibits angiotensin II-induced proliferation of vascular smooth muscle cells via regulating the expression of mitofusin 2. J. Huazhong Univ. Sci. Technol. Med. Sci. 2012, 32:31-35.
20. Marks A.R. Rapamycin: signaling in vascular smooth muscle. Transplant. Proc. 2003, 35:231S-233S.
21. Nagai K., Jiang M.H., Hada J., Nagata T., Yajima Y., Yamamoto S., et al. (-)-Epigallocatechin gallate protects against NO stress-induced neuronal damage after ischemia by acting as an anti-oxidant. Brain Res. 2002, 956:319-322.
22. Sachinidis A., Skach R.A., Seul C., Ko Y., Hescheler J., Ahn H.Y., et al. Inhibition of the PDGF beta-receptor tyrosine phosphorylation and its downstream intracellular signal transduction pathway in rat and human vascular smooth muscle cells by different catechins. FASEB J. 2002, 16:893-895.
23. Stangl V., Dreger H., Stangl K., Lorenz M. Molecular targets of tea polyphenols in the cardiovascular system. Cardiovasc. Res. 2007, 73:348-358.
24. Tipoe G.L., Leung T.M., Hung M.W., Fung M.L. Green tea polyphenols as an anti-oxidant and anti-inflammatory agent for cardiovascular protection. Cardiovasc. Hematol. Disord. Drug Target 2007, 7:135-144.
25. Weber A.A., Neuhaus T., Skach R.A., Hescheler J., Ahn H.Y., Schrör K., et al. Mechanisms of the inhibitory effects of epigallocatechin-3-gallate on platelet-derived growth factor-BB-induced cells ignaling and mitogenesis. FASEB J. 2004, 18:128-130.
26. Weisburger J.H., Chung F.L. Mechanisms of chronic disease causation by nutritional factors and tobacco products and their prevention by tea polyphenols. Food Chem. Toxicol. 2002, 40:1145-1154.
27. Willis A.I., Pierre-Paul D., Stunpio B.E., Gahtan V. Vascular smooth muscle cell migration: current research and clinical implications. Vasc. Endovascular Surg. 2004, 38:11-23.
28. Wu L., Li Z., Zhang Y., Zhang P., Zhu X., Huang J., et al. Adenovirus-expressed human hyperplasia suppressor gene induces apoptosis in cancer cells. Mol. Cancer Ther. 2008, 7:222-232.
29. Yamane T., Takahashi T., Kuwata K., Oya K., Inagake M., Kitao Y., et al. Inhibition of N-methyl-N-nitro-N-nitrosoguanidine-induced carcinogenesis by (-)-epigallocatechin gallate in the rat glandular stomach. Cancer Res. 1995, 55:2081-2084.
30. Yang J., Han Y., Sun H., Chen C., He D., Guo J., et al. (-)-Epigallocatechin gallate suppresses proliferation of vascular smooth muscle cells induced by high glucose by inhibition of PKC and ERK1/2 signalings. J. Agric. Food Chem. 2011, 59:11483-11490.
31. Zheng Y., Song H.J., Kim C.H., Kim H.S., Kim E.G., Sachinidis A., et al. Inhibitory effect of epigallocatechin 3-O-gallate on vascular smooth muscle cell hypertrophy induced by angiotensin II. J. Cardiovasc. Pharmacol. 2004, 43:200-208.
32. Zhou W., Chen K.H., Cao W., Zeng J., Liao H., Zhao L., et al. Mutation of the protein kinase A phosphorylation site influences the anti-proliferative activity of mitofusin 2. Atherosclerosis 2010, 211:216-223.