Use of transmission electron microscopy to identify nanocrystals of challenging protein targets

Proceedings of the National Academy of Sciences of the United States of America

Volumn 111, Issue 23, 2014, Pages 8470-8475

  Stevenson, Hilary P ^a   Makhov, Alexander M ^a   Calero, Monica ^a   Edwards, Andrea L ^b   Zeldin, Oliver B ^b   Mathews, Irimpan I ^c   Lin, Guowu ^a   Barnes, Christopher O ^a   Santamaria, Hugo ^a   Ross, Ted M ^d   Soltis, S Michael ^c   Khosla, Chaitan ^b   Nagarajan, V ^e   Conway, James F ^a   Cohen, Aina E ^c   Calero, Guillermo ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b STANFORD UNIVERSITY   (United States)

^c SLAC NATIONAL ACCELERATOR LABORATORY   (United States)

^d Vaccine and Gene Therapy Institute of Florida   (United States)

^e JAN Scientific Inc   (United States)

Author keywords

Crystal characterization; Crystal optimization; Femtosecond diffraction; Nanocrystal detection; Structural biology

Indexed keywords

NANOCRYSTAL; NANOPARTICLE; PROTEIN; TRYPTOPHAN;

ARTICLE; CRYSTAL; CRYSTALLIZATION; PRIORITY JOURNAL; SCREENING; STRUCTURE ANALYSIS; TRANSMISSION ELECTRON MICROSCOPY; X RAY; X RAY CRYSTALLOGRAPHY;

CRYSTAL CHARACTERIZATION; CRYSTAL OPTIMIZATION; FEMTOSECOND DIFFRACTION; NANOCRYSTAL DETECTION; STRUCTURAL BIOLOGY;

ANIMALS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; MICROSCOPY, ELECTRON, TRANSMISSION; NANOPARTICLES; PROTEINS; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; SF9 CELLS;

EID: 84902168263     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1400240111     Document Type: Article

References (26)

1. Aquila A, et al. (2012) Time-resolved protein nanocrystallography using an X-ray free-electron laser. Opt Express 20(3):2706-2716.
2. Boutet S, et al. (2012) High-resolution protein structure determination by serial femtosecond crystallography. Science 337(6092):362-364.
3. Kern J, et al. (2013) Simultaneous femtosecond X-ray spectroscopy and diffraction of photosystem II at room temperature. Science 340(6131):491-495.
4. Koopmann R, et al. (2012) In vivo protein crystallization opens new routes in structural biology. Nat Methods 9(3):259-262.
5. Redecke L, et al. (2013) Natively inhibited Trypanosoma brucei cathepsin B structure determined by using an X-ray laser. Science 339(6116):227-230.
6. Chapman HN, et al. (2011) Femtosecond X-ray protein nanocrystallography. Nature 470(7332):73-77.
7. Kern J, et al. (2012) Room temperature femtosecond X-ray diffraction of photosystem II microcrystals. Proc Natl Acad Sci USA 109(25):9721-9726.
8. Kissick DJ, Wanapun D, Simpson GJ (2011) Second-order nonlinear optical imaging of chiral crystals. Annu Rev Anal Chem (Palo Alto Calif) 4:419-437.
9. Haupert LM, Simpson GJ (2011) Screening of protein crystallization trials by second order nonlinear optical imaging of chiral crystals (SONICC). Methods 55(4):379-386.
10. Judge RA, Swift K, González C (2005) An ultraviolet fluorescence-based method for identifying and distinguishing protein crystals. Acta Crystallogr D Biol Crystallogr 61(Pt 1):60-66. (Pubitemid 41615531)
11. Wampler RD, et al. (2008) Selective detection of protein crystals by second harmonic microscopy. J Am Chem Soc 130(43):14076-14077.
12. Kadima W, McPherson A, Dunn MF, Jurnak FA (1990) Characterization of precrystallization aggregation of canavalin by dynamic light scattering. Biophys J 57(1):125-132. (Pubitemid 20033753)
13. Hunter MS, et al. (2011) X-ray diffraction from membrane protein nanocrystals. Biophys J 100(1):198-206.
14. Gill HS (2010) Evaluating the efficacy of tryptophan fluorescence and absorbance as a selection tool for identifying protein crystals. Acta Crystallogr Sect F Struct Biol Cryst Commun 66(Pt 3):364-372.
15. Du S, et al. (2011) Structure of the HIV-1 full-length capsid protein in a conformationally trapped unassembled state induced by small-molecule binding. J Mol Biol 406(3):371-386.
16. Desbois S, Seabrook SA, Newman J (2013) Some practical guidelines for UV imaging in the protein crystallization laboratory. Acta Crystallogr Sect F Struct Biol Cryst Commun 69(Pt 2):201-208.
17. Hunter MS, Fromme P (2011) Toward structure determination using membrane-protein nanocrystals and microcrystals. Methods 55(4):387-404.
18. Nederlof I, Li YW, van Heel M, Abrahams JP (2013) Imaging protein three-dimensional nanocrystals with cryo-EM. Acta Crystallogr D Biol Crystallogr 69(Pt 5):852-859.
19. Shi D, Nannenga BL, Iadanza MG, Gonen T (2013) Three-dimensional electron crystallography of protein microcrystals. Elife 2:e01345.
20. DePonte DP, et al. (2008) Gas dynamic virtual nozzle for generation of microscopic droplet streams. J Phys D Appl Phys 41(19):195505.
21. Pullara F, et al. (2013) A general path for large-scale solubilization of cellular proteins: From membrane receptors to multiprotein complexes. Protein Expr Purif 87(2):111-119.
22. Wong FT, Jin X, Mathews II, Cane DE, Khosla C (2011) Structure and mechanism of the trans-acting acyltransferase from the disorazole synthase. Biochemistry 50(30):6539-6548.
23. Takagi Y, et al. (2006) Head module control of mediator interactions. Mol Cell 23(3):355-364. (Pubitemid 44128845)
24. Dieryck W, Noubhani AM, Coulon D, Santarelli X (2003) Cloning, expression and two-step purification of recombinant His-tag enhanced green fluorescent protein overexpressed in Escherichia coli. J Chromatogr B Analyt Technol Biomed Life Sci 786(1-2):153-159. (Pubitemid 36324571)
25. Boutet Sb & Williams GJ (2010) The coherent x-ray imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). New J Phys 12(3):035024.
26. Sauter NK, Hattne J, Grosse-Kunstleve RW, Echols N (2013) New Python-based methods for data processing. Acta Crystallogr D Biol Crystallogr 69(Pt 7):1274-1282.